ER stress modulates apoptosis in A431 cell subjected to EtNBSe-PDT via the PERK pathway

Chen, Jing; Zhou, Dawei; Kang, Jian; Liu, Chenxi; Huang, Rou-Jie; Jiang, Zhengqian; Liao, Yuxuan; Liu, An; Gao, Lihua; Song, Xiangzhi; Chen, Yihui; Wang, Hongyi; Lan, Zehao; Wang, Weidong; Guan, Haoyu; Chen, Xiang; Huang, Jinhua

doi:10.1016/j.pdpdt.2021.102305

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…PDT and PTT can induce cell death through apoptosis, necrosis, and autophagy pathways. 43 The lower left quadrant of flow cytometry images is the live cell population, the lower right quadrant is the early apoptotic (annexin V-FITC positive) population, the upper left quadrant is the necrotic cell (annexin V-FTIC negative) population, and the upper right is the late apoptotic population. 44 The flow cytometry (annexin V-FITC/PI staining method) was utilized to quantitatively verify whether NPs-mediated PDT/PTT can induce cell apoptosis.…”

Section: Resultsmentioning

confidence: 99%

PpIX/IR-820 Dual-Modal Therapeutic Agents for Enhanced PDT/PTT Synergistic Therapy in Cervical Cancer

et al. 2022

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High treatment accuracy is the key to efficient cancer treatment. Photodynamic therapy (PDT) and photothermal therapy (PTT) are two kinds of popular, precise treatment methods. The combination of photodynamic and photothermal therapy (PDT/PTT) can greatly enhance the precise therapeutic efficacy. In this work, protoporphyrin IX (PpIX) was selected as the PDT agent (photosensitizer), and new indocyanine green (IR-820) was selected as the PTT agent. Further, the two kinds of theranostic agents were encapsulated by biological-membrane-compatible liposomes to form PpIX-IR-820@Lipo nanoparticles (NPs), a new kind of PDT/PTT agent. The PpIX-IR-820@Lipo NPs exhibited good water solubility, a spherical shape, and high fluorescence peak emission in the near-infrared spectral region (700–900 nm, NIR). The cellular toxicity of PpIX-IR-820@Lipo NPs for human cervical cancer cells (HeLa) and human cervical epithelial cells (H8) was detected by the CCK-8 method, and low cytotoxicity was observed for the PpIX-IR-820@Lipo NPs. Then, the excellent cellular uptake of PpIX-IR-820@Lipo NPs was confirmed by laser scanning confocal microscopy. Moreover, the PDT/PTT property of PpIX-IR-820@Lipo NPs was illustrated via 2′,7′-dichlorofluorescin diacetate (DCFH-DA) and annexin V-fluorescein isothiocyanate (annexin V-FITC), as indicator probes. The PDT/PTT synergistic efficiency of PpIX-IR-820@Lipo NPs on HeLa cells was verified, exhibiting a high efficiency of 70.5%. Thus, the novel theranostic PpIX-IR-820@Lipo NPs can be used as a promising PDT/PTT synergistic theranostic nanoplatform in future cervical cancer treatment.

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Section: Resultsmentioning

confidence: 99%

PpIX/IR-820 Dual-Modal Therapeutic Agents for Enhanced PDT/PTT Synergistic Therapy in Cervical Cancer

et al. 2022

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“…The third mechanism involves the activation of the immune response against cancer cells through acute inflammatory processes and the release of cytokines into the tumor. The advantage of one pathway over the other depends on the parameters used in therapy and the patient's health condition [102][103][104][105][106][107][108].…”

Section: Mechanism Of Tumor Destruction Using the Pdt Methodsmentioning

confidence: 99%

Current Photodynamic Therapy for Glioma Treatment: An Update

Aebisher,

Przygórzewska,

Myśliwiec

et al. 2024

Biomedicines

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Research on the development of photodynamic therapy for the treatment of brain tumors has shown promise in the treatment of this highly aggressive form of brain cancer. Analysis of both in vivo studies and clinical studies shows that photodynamic therapy can provide significant benefits, such as an improved median rate of survival. The use of photodynamic therapy is characterized by relatively few side effects, which is a significant advantage compared to conventional treatment methods such as often-used brain tumor surgery, advanced radiotherapy, and classic chemotherapy. Continued research in this area could bring significant advances, influencing future standards of treatment for this difficult and deadly disease.

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“…Based on our previous research, 400 nmol/L of EtNBSe (Department of Chemistry of Central South University, China) was used to pretreat all groups in the dark for 20 min. Then the cells were irradiated by LED light whose wavelength was 635 nm and power density of 50 mW/cm 2 for 60 s (2.8 J/cm 2 ) ( 25 , 26 ). After corresponding treatments, cells were harvested and fixed with 75% ethanol overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Identification and validation of four photodynamic therapy related genes inhibiting MAPK and inducing cell cycle alteration in squamous cell carcinoma

et al. 2022

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IntroductionCutaneous squamous cell carcinoma (cSCC) is the second most common skin cancer, and photodynamic therapy (PDT) is a promising modality against cSCC. This study investigated the impact of PDT on the MAPK pathway and cell cycle alternation of cSCC as well as the related molecular mechanisms.MethodExpressing mRNA profile data sets GSE98767, GSE45216, and GSE84758 were acquired from the GEO database. The functions of differently expressed genes (DEGs) were enriched by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Least absolute shrinkage and selection operator (Lasso) analysis were used to establish a diagnosis model based on GSE98767. A correlation analysis and a protein–protein interaction (PPI) network were used to evaluate the relationship between cSCC-PDT-related genes and the MAPK pathway. Single-sample gene set enrichment analysis (ssGSEA) was performed on GSE98767 to estimate MAPK activation and cell cycle activity. Finally, the effect of MAPK activation on the cell cycle was explored in vitro.ResultFour cSCC-PDT-related genes, DUSP6, EFNB2, DNAJB1, and CCNL1, were identified as diagnostic markers of cSCC, which were upregulated in cSCC or LC50 PDT-protocol treatment and negatively correlated with the MAPK promoter. Despite having a smaller MAPK activation score, cSCC showed higher cell cycle activity. The PDT treatment suppressed the G1 to G2/M phase in JNK overexpressed A431 cells.ConclusionCCNL1, DNAJB1, DUSP6, and EFNB2 were identified as potential PDT target genes in cSCC treatment, whose potential therapeutic mechanism was inhibiting the MAPK pathway and inducing cell cycle alternation.

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ER stress modulates apoptosis in A431 cell subjected to EtNBSe-PDT via the PERK pathway

Cited by 4 publications

References 36 publications

PpIX/IR-820 Dual-Modal Therapeutic Agents for Enhanced PDT/PTT Synergistic Therapy in Cervical Cancer

PpIX/IR-820 Dual-Modal Therapeutic Agents for Enhanced PDT/PTT Synergistic Therapy in Cervical Cancer

Current Photodynamic Therapy for Glioma Treatment: An Update

Identification and validation of four photodynamic therapy related genes inhibiting MAPK and inducing cell cycle alteration in squamous cell carcinoma

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