ErbB1 and ErbB2 overexpression in patients with sinonasal inverted papilloma and inverted papilloma with squamous cell carcinoma in China

Li, Hongbing; Hu, Li; Zhang, Huankang; Wang, Dehui

doi:10.1080/00016489.2019.1650200

Cited by 4 publications

(4 citation statements)

References 18 publications

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“…7 B,C). In addition, significantly increased of mRNA and protein expression level of EGFR was found in SIP compared to normal control and the expression level was related with dysplastic change of the cell 56 , consistant with our result that higher expression of EGFR was found in both IP4 tissue and cells derived from it compared to NP tissue and NP cells, respectively (Fig. 7 B,C).…”

Section: Discussionsupporting

confidence: 89%

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

Nukpook

Ekalaksananan

Kiyono

et al. 2021

Sci Rep

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To better understand the pathogenesis of nasal polyps (NPs) and sinonasal inverted papillomas (SIPs), we aimed to establish cell lines from fresh tissues of NPs and SIPs and characterize them. Primary cell cultures were obtained from two NP tissues (NP2 and NP3) and one SIP tissue (IP4). All the cells were polygonal in shape, expressed cytokeratin 14, and had normal diploid chromosome status. HPV58 DNA was detected in NP3. To obtain immortal primary cells, NP2 and IP4 cells were transduced with a combination of mutant CDK4, cyclinD1 and TERT. These cells were thereafter named NP2/K4DT and IP4/K4DT, respectively. HPV58-positive NP3 cells were transduced with TERT alone, the resulting cells named NP3/T. Phenotypic and genotypic identity of original tissues and derived cells was investigated. All the cell cultures with transgenes were confirmed to be derived from their parental cells and primary tumor tissues by analysis of short tandem repeats (STR) and maintained in vitro growth, genetic profiles and gene expression characteristics of the primary cells. These virtually immortalized cells, as well as the primary cells, have potential as in vitro models for studying the pathogenesis of NPs and SIPs and for preclinical study to develop new therapeutic agents.

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Section: Discussionsupporting

confidence: 89%

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

Nukpook

Ekalaksananan

Kiyono

et al. 2021

Sci Rep

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“…Recent studies have better characterized the molecular alterations, IHC features, and expression profiles of sinonasal papillomas. 18,19 Despite this, there are no reliable distinguishing IHC markers. While up to 90% of patients with IP will harbor activating EGFR mutations, 4-6 mutation analysis is impractical in clinical practice, and there are no immunostains or in situ hybridization assays that can exploit the EGFR pathway upregulation that drives their growth for diagnostic purposes.…”

Section: Discussionmentioning

confidence: 99%

“…Diagnostic uncertainty may lead to substandard patient management or the need for an intraoperative frozen section, which can be avoided when a definitive diagnosis is rendered on an “in office” biopsy specimen. Recent studies have better characterized the molecular alterations, IHC features, and expression profiles of sinonasal papillomas 18,19. Despite this, there are no reliable distinguishing IHC markers.…”

Section: Discussionmentioning

confidence: 99%

CD163 Immunohistochemical “Circle Sign” Staining Pattern Differentiates Sinonasal Papillomas From Morphologically Similar Non-neoplastic Lesions

Sterling

Mehrad

Ely

et al. 2022

American Journal of Surgical Pathology

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Sinonasal papillomas are a diverse group of benign epithelial neoplasms of the sinonasal tract. Inverted papilloma, in particular, must be distinguished from other lesions with no malignant potential. The aim of this study was to distinguish sinonasal papillomas from morphologically similar lesions using CD163 immunostaining. Cases from a 19-year period were identified. These included 49 inverted, 10 exophytic, and 12 oncocytic papillomas, 21 chronic sinusitides with squamous metaplasia, 27 inflammatory polyps, 5 verrucae vulgares, 5 respiratory epithelial adenomatoid hamartomas, and 6 DEK:: AFF2 carcinomas of the sinonasal tract. A subset of biopsy cases (8 inverted papillomas, 5 inflammatory polyps) was separately analyzed. CD163 immunohistochemistry (IHC) was performed. A unique "circle" staining pattern was identified in the surface epithelium. After locating a hotspot, circles were quantified in 10 consecutive high-power fields. Circles were present in 66/71 (93%) cases of sinonasal papilloma, with a mean of 35 circles/ 10 HPF (range: 0 to 160/10 HPF) and a median of 19 circles/ 10 HPF. Circles were present in 20/58 (34%) non-neoplastic cases, with a mean of 2 circles/10 HPF (range: 0 to 27/10 HPF) and a median of 0. Considering all resection and biopsy cases, performance for distinguishing papillomas from non-neoplastic lesions was best at a cutoff of 10 circles/10 HPF (2-tailed P < 0.0001) with sensitivity, specificity, positive predictive value, and negative predictive value of 66.2%, 93.1%, 92.1%, and 69.2%, respectively. The results were similar in the biopsy subset. One other neoplastic entity, the DEK::AFF2 carcinomas, also showed prominent CD163 circle staining. In summary, si-nonasal papillomas demonstrate extensive CD163 "circle" staining in the epithelium compared with the nonneoplastic lesions studied. As such, the "circle sign" on CD163 IHC may be helpful in distinguishing between diagnoses, particularly on small biopsies or equivocal specimens.

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“…However, there have been few studies focused on etiology and pathogenesis. One recent study suggested the potential role of chronic in ammation [6,7] and viral (human papillomavirus and Epstein-Barr virus) infection in the etiology of SNIP [8][9][10]. At the molecular level, several cell cycle and angiogenic factors, such as Ki67, vascular endothelial growth factor (VEGF), and Akt/mTOR have been implicated in the development and progression of SNIP [11].…”

Section: Introductionmentioning

confidence: 99%

Identification and Potential Value of Candidate Genes in Patients with Sinonasal inverted papilloma

Chen

2023

Preprint

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Background: This study aimed to explore candidate genes involved in the pathogenesis of sinonasal inverted papilloma (SNIP) using bioinformatic analysis and experimental verification. Methods: For this, gene expression profiles (GSE193016) were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were identified, with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) being used to analyze the functions and pathways of DEGs. Co-expression networks were established using single-sample gene set enrichment analysis (GSEA). Furthermore, Search Tool for the Retrieval of Interacting Genes (STRING), Cytoscape, Molecular Complex Detection (MCODE), and Cytohubba were used to construct protein–protein interaction (PPI) networks, screen hub DEGs, and identify hub genes, with a miRNA-TF-hub gene regulatory network also being constructed. Finally, expression levels of identified hub genes were verified using RT-qPCR. Results: As a result, 985 DEGs were identified, including 343 upregulated and 642 downregulated genes. Functional enrichment analyses showed that upregulated DEGs were significantly enriched in epidermal development, cornification, keratinocyte differentiation, epidermal cell differentiation, and skin development. Meanwhile, downregulated DEGs were significantly enriched in muscle contraction, extracellular structure organization, muscle system process, extracellular matrix organization, and regulation of membrane potential. The top eight hub genes were MAD2L1, CDC20, CCNB2, CCNB1, AURKB, NDC80, KIF11, and DLGAP5. During the validation stage, the expression of MAD2L1 was significantly higher in patients with SNIP than in those with normal nasal mucosa. Conclusions: Overall, this study identified key genes associated with SNIP and their biological functions. Furthermore, a variety of predicted genes involved in the cell cycle were found to be connected to SNIP pathogenesis and malignant transformation. MAD2L1may play an important role in SNIP pathogenesis, providing novel insights into the occurrence and development of SNIP.

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ErbB1 and ErbB2 overexpression in patients with sinonasal inverted papilloma and inverted papilloma with squamous cell carcinoma in China

Cited by 4 publications

References 18 publications

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

Establishment and genetic characterization of cell lines derived from proliferating nasal polyps and sinonasal inverted papillomas

CD163 Immunohistochemical “Circle Sign” Staining Pattern Differentiates Sinonasal Papillomas From Morphologically Similar Non-neoplastic Lesions

Identification and Potential Value of Candidate Genes in Patients with Sinonasal inverted papilloma

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