ERDR1 enhances human NK cell cytotoxicity through an actin-regulated degranulation-dependent pathway

Lee, Ha‐Reum; Huh, Scarlett Yoona; Hur, Dae Young; Jeong, Hyunjo; Kim, Tae Sung; Kim, Sang Yoon; Park, Seung Beom; Yang, Yoolhee; Bang, Sa Ik; Park, Hyun-Jeong; Cho, Daeho

doi:10.1016/j.cellimm.2014.10.002

Cited by 11 publications

(17 citation statements)

References 44 publications

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“…Erdr1 increases growth of intestinal organoids. Previous studies have reported that Erdr1 is involved in NK cell activation 31 , Tcell apoptosis 23 , and melanoma migration 26 . However, the role of Erdr1 in the intestine remains unknown.…”

Section: Resultsmentioning

confidence: 99%

Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration

et al. 2020

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Gut microbiota and their metabolites are instrumental in regulating intestinal homeostasis. However, early-life microbiota associated influences on intestinal development remain incompletely understood. Here we demonstrate that co-housing of germ-free (GF) mice with specific-pathogen free (SPF) mice at weaning (exGF) results in altered intestinal gene expression. Our results reveal that one highly differentially expressed gene, erythroid differentiation regulator-1 (Erdr1), is induced during development in SPF but not GF or exGF mice and localizes to Lgr5 + stem cells and transit amplifying (TA) cells. Erdr1 functions to induce Wnt signaling in epithelial cells, increase Lgr5 + stem cell expansion, and promote intestinal organoid growth. Additionally, Erdr1 accelerates scratch-wound closure in vitro, increases Lgr5 + intestinal stem cell regeneration following radiation-induced injury in vivo, and enhances recovery from dextran sodium sulfate (DSS)-induced colonic damage. Collectively, our findings indicate that early-life microbiota controls Erdr1-mediated intestinal epithelial proliferation and regeneration in response to mucosal damage.

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Section: Resultsmentioning

confidence: 99%

Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration

et al. 2020

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“…Since these genes sit very close to the Ifi204 gene on the chromosome, it is speculated that reduced expression of p205 and Pydc3 may be attributable to their impaired transcription resultant of deleting the Ifi204 gene. The expression of ERDR1, a protein shown to enhance natural killer (NK) cell cytotoxicity 29 , was reduced in p204 KO mice, indicating that p204 may be important for NK cell mediated anti-tumor immunity.…”

Section: Resultsmentioning

confidence: 99%

RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity

Jian

Wei

Yin

et al. 2018

Sci Rep

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p204, a murine member of the interferon-inducible p200 protein family, and its human analogue, IFI16, have been shown to function as tumor suppressors in vitro, but the molecular events involved, in particular in vivo, remain unclear. Herein we induced the Lewis Lung carcinoma (LLC) murine model of human lung cancer in p204 null mice (KO) and their control littermates (WT). We compared the transcriptome in spleen from WT and p204 KO mice using a high-throughput RNA-sequencing array. A total 30.02 Gb of clean data were obtained, and overall Q30% was greater than 90.54%. More than 75% of clean data from 12 transcriptome samples were mapped to exons. The results showed that only 11 genes exhibited altered expression in untreated p204 KO mice relative to untreated WT mice, while 393 altered genes were identified in tumor-bearing p204 KO mice when compared with tumor-bearing WT mice. Further differentially expressed gene cluster and gene ontology consortium classification revealed that numerous cytokines and their receptors, chemoattractant molecules, and adhesion molecules were significantly induced in p204 KO mice. This study provides novel insights to the p204 network in anti-tumor immune response and also presents a foundation for future work concerning p204-mediated gene expressions and pathways.

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“…Also, Erdr1 exhibits these functions not only in cancer cells but also in immune cells. Treatment of natural killer cells with recombinant Erdr1 enhances its cytotoxicity via the secretion of lytic granules [ 8 ], suggesting that this treatment increases the killing of cancer cells by these immune cells.…”

Section: Introductionmentioning

confidence: 99%

Erdr1 Suppresses Murine Melanoma Growth via Regulation of Apoptosis

Lee

Jung

Park

et al. 2016

IJMS

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Melanoma, one of the aggressive cancers, is known to be resistant to chemotherapy. Because of its aggressive nature, effectively inducing apoptosis is necessary to treat melanoma. Erythroid differentiation regulator 1 (Erdr1) is known to be a stress-related survival factor exhibiting anti-cancer effects in several cancers. However, little is known about the functions and underlying mechanisms of Erdr1 so far. To demonstrate the effect of Erdr1 in melanoma apoptosis, recombinant murine Erdr1 was injected into mice implanted with B16F10 melanoma cells. In vivo tumor growth was significantly inhibited in mice injected with Erdr1 compared to the control. In addition, the tumor from Erdr1-injected mice showed an increased level of apoptosis. Accordingly, apoptosis-regulating factors including anti-apoptotic marker Bcl-2 and pro-apoptotic marker Bax in the tumor tissues were examined. As expected, the decreased level of Bcl-2 and increased level of Bax were detected in tumors within the mice injected with Erdr1. Based on the in vivo study, the role of Erdr1 in tumor apoptosis was further tested by incubating it with cells of the murine melanoma cell line B16F10. Erdr1-induced apoptosis in B16F10 cells was observed. Additionally, Erdr1 downregulated STAT3 activity, inhibiting apoptosis via regulation of the Bcl-2 family. Overall, data demonstrate that Erdr1 induced murine melanoma apoptosis through the regulation of Bcl-2 and Bax. These findings suggest that Erdr1 is a novel regulator of apoptosis in melanoma.

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ERDR1 enhances human NK cell cytotoxicity through an actin-regulated degranulation-dependent pathway

Cited by 11 publications

References 44 publications

Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration

Erythroid differentiation regulator-1 induced by microbiota in early life drives intestinal stem cell proliferation and regeneration

RNA-Seq analysis of interferon inducible p204-mediated network in anti-tumor immunity

Erdr1 Suppresses Murine Melanoma Growth via Regulation of Apoptosis

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