Ergothioneine Production by Submerged Fermentation of a Medicinal Mushroom Panus conchatus

Abstract

Ergothioneine is a natural and safe antioxidant that plays an important role in anti-aging and the prevention of various diseases. This study aimed to report on a kind of medicinal mushroom of Panus conchatus with great potential for the bioproduction of ergothioneine. The effect of different nutritional and environmental conditions on the growth of Panus conchatus and ergothioneine production were investigated. Molasses and soy peptone were found to promote cell growth of Panus conchatus and enhance ergothioneine accumulation. Adding precursors of histidine, methionine and cysteine could increase ergothioneine production and the highest ergothioneine concentration of 148.79 mg/L was obtained. Finally, the extraction and purification processes were also established to obtain the crude ergothioneine extract for further antioxidant property evaluation. The ergothioneine from Panus conchatus showed high antioxidant activity with good stability in a lower pH environment. This study provided a new strain and process for the bioproduction of ergothioneine.

Effects of Drying Process and High Hydrostatic Pressure on Extraction of Antioxidant Ergothioneine from Pleurotus citrinopileatus Singer

“…In fungi, the synthesis of ergothioneine depends on the glutathione content and its precursors, and the EGT content varies greatly depending on the strain and growth conditions [ 8 ]. Different extraction, synthesis, and purification methods may contribute to the reported EGT concentrations [ 9 , 10 , 11 , 12 ].…”

“…In fungi, the synthesis of ergothioneine depends on the glutathione content and its precursors, and the EGT content varies greatly depending on the strain and growth conditions [8]. Different extraction, synthesis, and purification methods may contribute to the reported EGT concentrations [9][10][11][12]. Pleurotus citrinopileatus Singer (Figure 1B) is rich in EGT [13], and its efficient development and utilization hold promise as a potential source of human EGT supplements.…”