Eriocalyxin B induces apoptosis and cell cycle arrest in pancreatic adenocarcinoma cells through caspase- and p53-dependent pathways

Li, Lin; Yue, Grace Gar-Lee; Lau, Clara Bik‐San; Sun, Han‐Dong; Fung, Kwok‐Pui; Leung, Ping Chung; Han, Quan‐Bin; Leung, Po Sing

doi:10.1016/j.taap.2012.04.021

Cited by 44 publications

(35 citation statements)

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“…EriB was observed to be involved in the down regulation of Bcl-2 in dose-dependent manner ( Figure 5). These results are similar with previously reported studies in pancreatic adenocarcinoma (Li et al, 2012).…”

Section: Resultssupporting

confidence: 94%

“…The results of flow cytometric analysis showed that rates of apoptosis were 17.6 ± 2.1 and 24.9 ± 2.5% in the cells treated with 3 and 5 μM of EriB respectively for 24 hours as compared to 5.9 ± 1.0% in the control cells (Figure 4). EriB-induced apoptosis in T24 cells was consistent with previously reported studies in ovarian cancer (Leizer et al, 2010), pancreatic adenocarcinoma (Li et al, 2012) and leukemia (Wang et al, 2007;Zhang et al, 2010).…”

Section: Resultssupporting

confidence: 91%

“…Therefore, we analyzed effect of EriB on cell cycle progression of T24 cells. It was found that EriB arrested cell cycle at G2/M phase, which supported the previous reported results in pancreatic adenocarcinoma (Li et al, 2012). The percentage of accumulation of cells in the G2/M phase was increased from 15.97% in control group to 25.64%, and 38.48% in the cells treated with 3 and 5 μM of EriB respectively for 24 hours (Figure 3).…”

Section: Resultssupporting

confidence: 90%

“…Furthermore, the cells become round-shaped and poorly adhered to the cultured plates while the control group cells showed a typical polygonal and cobblestone monolayer appearance and remained firmly attached to cultured plates (data not shown). The results indicated that EriB induced growth inhibition of T24 cells, in addition to other type of cancer cells previously reported including ovarian cancer (Leizer et al, 2010), pancreatic adenocarcinoma (Li et al, 2012) and leukemia (Wang et al, 2007;Zhang et al, 2010). …”

Section: Resultssupporting

confidence: 63%

“…laxiflora, a herb of the Labiatae family distributed in the South-West China and has been reported to have wide spectrum of biological effects, including anti-inflammatory and antibacterial agent in local folk medicine (Ikezoe et al, 2003;Wang et al, 2007). Furthermore, EriB has anti-proliferative effect and induced apoptosis in cancer cells such as ovarian cancer (Leizer et al, 2010), pancreatic adenocarcinoma (Li et al, 2012) and leukemia (Wang et al, 2007;Zhang et al, 2010). However, the cytotoxic effects of EriB on bladder cancer and its mechanism were still unknown.…”

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confidence: 99%

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Eriocalyxin B inhibits proliferation and induces apoptosis through downregulation of Bcl-2 and activation of caspase-3 in human bladder cancer cells

Rasul

Liu

et al. 2013

Bangladesh J Pharmacol

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Eriocalyxin B inhibits proliferation and induces apoptosis through down -regulation of Bcl-2 and activation of caspase-3 in human bladder cancer cells Bangladesh Journal of Pharmacology Research Article IntroductionNatural products provide many promising sources of potential anti-cancer agents and several lead structures in the past decades, which were originally isolated from plants such as paclitaxel, camptothecin, vinca alkaloids, and etoposide have potential application in cancer chemotherapy, therefore, plants are considered as one of the most vital sources for the development of novel anti-cancer drugs (Amin et al., 2009;Cragg and Newman, 2005). Diterpenoids constitute a vast class of isoprenoid natural products, which are found mainly in plants and fungi and also been found in marine organisms and insects as well (Garcia et al., 2007;Hanson, 2004). Eriocalyxin B (EriB) is a natural entkaurene diterpene compound isolated from Isodon eriocalyx var. laxiflora, a herb of the Labiatae family distributed in the South-West China and has been reported to have wide spectrum of biological effects, including anti-inflammatory and antibacterial agent in local folk medicine (Ikezoe et al., 2003;Wang et al., 2007). Furthermore, EriB has anti-proliferative effect and induced apoptosis in cancer cells such as ovarian cancer (Leizer et al., 2010), pancreatic adenocarcinoma (Li et al., 2012) and leukemia (Wang et al., 2007;Zhang et al., 2010). However, the cytotoxic effects of EriB on bladder cancer and its mechanism were still unknown.Bladder cancer is an increasingly common and potentially lethal malignancy (Ploeg et al., 2009 AbstractEriocalyxin B (EriB) has been shown to possess promising anti-cancer potential against various cancer cells. However, its effect against bladder cancer cells remained unexplored. In this study, for the first time, we investigated the effects of EriB on cell proliferation, cell cycle, and apoptosis in bladder cancer T24 cells. In order to examine the effects of EriB on cell proliferation, cell cycle, and apoptosis, we performed MTT assay, flow cytometric analysis and Western blot. The results revealed that EriB decreased the cell viability of T24 cells. Flow cytometric analysis demonstrated that EriB markedly induced apoptosis of T24 cells and arrested cell cycle at G2/M phase in a dose-dependent manner. Further characteriza-tion showed that EriB involved in the down-regulation of Bcl-2 and activation of caspase-3 before culminating in apoptosis in EriB-treated T24 cells. These in vitro results suggested that EriB should be further examined for in vivo activity in human bladder cancer. This ork is li e sed u der a Creati e Co o s Attri utio 3.0 Li e se. You are free to opy, distri ute a d perfor the ork. You ust attri ute the ork i the a er spe ified y the author or li e sor. Article Info

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Section: Resultssupporting

confidence: 94%

Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 63%

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confidence: 99%

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Eriocalyxin B inhibits proliferation and induces apoptosis through downregulation of Bcl-2 and activation of caspase-3 in human bladder cancer cells

Rasul

Liu

et al. 2013

Bangladesh J Pharmacol

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Total Synthesis of Sculponin U through a Photoinduced Radical Cascade Cyclization

et al. 2023

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We have accomplished the total synthesis of sculponin U, a polycyclic C‐20‐oxygenated kaurane diterpenoid featuring a 7,20‐lactone‐hemiketal bridge, through a radical cascade cyclization triggered by photoinduced electron transfer (PET) of a silyl enolate to form the cyclohexanone‐fused bicyclo[3.2.1]octane skeleton. Other key points in our synthetic strategy encompass a Diels–Alder reaction to construct the middle six‐membered ring of sculponin U, and an intramolecular radical cyclization induced by iron‐catalyzed hydrogen atom transfer to close the western cyclohexane ring. Successful preparation of the enantiopure silyl enolate as the PET precursor enables the asymmetric total synthesis of sculponin U, opening a new avenue for divergent syntheses of structurally related C‐20‐oxygenated kaurane congeners and pharmaceutical derivatives thereof.

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Total Synthesis of Sculponin U through a Photoinduced Radical Cascade Cyclization

et al. 2023

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We have accomplished the total synthesis of sculponin U, a polycyclic C-20-oxygenated kaurane diterpenoid featuring a 7,20-lactone-hemiketal bridge, through a radical cascade cyclization triggered by photoinduced electron transfer (PET) of a silyl enolate to form the cyclohexanone-fused bicyclo[3.2.1]octane skeleton. Other key points in our synthetic strategy encompass a Diels-Alder reaction to construct the middle six-membered ring of sculponin U, and an intramolecular radical cyclization induced by iron-catalyzed hydrogen atom transfer to close the western cyclohexane ring. Successful preparation of the enantiopure silyl enolate as the PET precursor enables the asymmetric total synthesis of sculponin U, opening a new avenue for divergent syntheses of structurally related C-20-oxygenated kaurane congeners and pharmaceutical derivatives thereof.

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Eriocalyxin B induces apoptosis and cell cycle arrest in pancreatic adenocarcinoma cells through caspase- and p53-dependent pathways

Cited by 44 publications

References 63 publications

Eriocalyxin B inhibits proliferation and induces apoptosis through downregulation of Bcl-2 and activation of caspase-3 in human bladder cancer cells

Eriocalyxin B inhibits proliferation and induces apoptosis through downregulation of Bcl-2 and activation of caspase-3 in human bladder cancer cells

Total Synthesis of Sculponin U through a Photoinduced Radical Cascade Cyclization

Total Synthesis of Sculponin U through a Photoinduced Radical Cascade Cyclization

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