EROD activity and cytochrome P4501A induction in liver and gills of Senegal sole Solea senegalensis from a polluted Huelva Estuary (SW Spain)

Abstract

EROD activity and induction cytochrome P4501A in liver and gills of Senegal sole, Solea senegalensis, from a heavy metal and PAH polluted estuary, was studied. Liver and gill CYP1A catalytic activity was assessed at the enzyme activity level-measured as 7-ethoxyresorufin-O-deethylase and cellular localization of CYP1A in the liver was studied using immunohistochemistry. Liver EROD was correlated with phenanthrene-type metabolites in liver and copper concentrations in water. Strong CYP1A occurrence was observed in acinar pancreatic cells, pancreatic duct epithelium and vascular system endothelium and negative/rare induction were observed in hepatocytes and sinusoidal endothelium. In gills, EROD activity showed a significant correlation with different fractions of heavy metals in sediment but no correlation was observed between EROD activity and PAHs. Strongly positive CYP1A associated staining of the vascular system endothelia and primary filament cells and a moderate staining of pillar cells in gills were observed. The results substantiated the utility of EROD activity and CYP1A induction measurement as biomarkers for use by aquatic toxicologists and indicate that catalytic assays and immunohistochemical assays appear to be sensitive to different kinds of pollutants being the use of both methods recommended for monitoring programs.

Benzo(a)pyrene Metabolism and EROD and GST Biotransformation Activity in the Liver of Red- and White-Blooded Antarctic Fish

“…65,66 Thus, it may be mainly the thermal inflexibility of cytochrome P4501A (reflected by EROD activity) that could limit the xenobiotics metabolism capability of Antarctic fish during future seawater warming and pollution.…”

Assessing pollution in marine protected areas: the role of a multi-biomarker and multi-organ approach

“…Biomarkers combined with metal body burdens of resident organisms have been widely used in aquatic pollution monitoring (Van der Oost et al 2003;Au 2004;Giarratano et al 2010;Duarte et al 2011;Oliva et al 2012Oliva et al , 2014Barhoumi et al 2014;Morachis-Valdez et al 2015), though most biomarker-based environmental quality studies were performed exclusively in highly anthropized sites (Malins et al 2006;Ramos-Gómez et al 2011;Ben Ameur et al 2012;Maranho et al 2012;Pereira et al 2014). We hypothesize that biomarkers are adequate for assessing the environmental quality of MPAs.…”

Effects of chronic exposure to tributyltin on tissue-specific cytochrome P450 1 regulation in juvenile common carp

“…Thus, over the past decade, an increasing number of studies have explored the possibility of using CYP450 system as biomarkers of adverse effects at the cellular level (Eggens et al, 1995;Jonsson et al, 2003;Oliva et al, 2014). Studies on the induction of CYP450 systems in fish have focused primarily in the liver.…”

“…The CYP450 system is responsible for the metabolism of a wide range of endogenous and xenobiotic compounds, as it plays important roles in the toxicology, metabolism and excretion of pollutants, drugs and many carcinogenic chemicals and mediates the transformation of certain xenobiotics to their reactive intermediates (Nahrgang et al, 2010;Oliva et al, 2014). Thus, over the past decade, an increasing number of studies have explored the possibility of using CYP450 system as biomarkers of adverse effects at the cellular level (Eggens et al, 1995;Jonsson et al, 2003;Oliva et al, 2014).…”

“…Moreover, the application of cellular and molecular biomarkers in ecotoxicology depends upon basic information on xenobiotic biotransformation mechanisms (Oliva et al, 2014). Among them, the cytochrome P450 (CYP450) systems are very popular biomarker applying in the ecotoxicological field (Burkina et al, 2015).…”