Erratum: Corrigendum: Genetic improvement of tomato by targeted control of fruit softening

Abstract

In the version of this article initially published, on p.369, "European consumers" were said to account for "over €1.1 ($1.21) million" of the "nearly €1.9 million worldwide gluten-free food market"; in both cases the figure should have been in billions, not millions. In addition, the opening sentence said "This winter, " but should have read "Soon, " and on p.371, paragraph three, "at a General Mills facility earlier this year, " should have read "in 2015. " The errors have been corrected in the HTML and PDF versions of the article.

The SlFSR gene controls fruit shelf-life in tomato

“…This process is stimulated by various cell wall modifying enzymes and proteins, including PG, CEL, TBG, XYL, XTH, and PE, and other cell wall loosening proteins, such as EXP ( Brummell and Harpster, 2001 ). To investigate whether the expression of cell wall modification-related genes differs between SlFSR -RNAi and WT fruits, transcripts of PE ( Phan et al , 2007 ), PG ( Giovannoni et al , 1989 ), CEL2 ( Lashbrook et al , 1994 ), XYL1 ( Buanafina et al , 2015 ), XTH5 ( Miedes and Lorences, 2009 ), TBG4 ( Smith et al , 2002 ), MAN1 ( Meli et al , 2010 ), PL ( Uluisik et al , 2016 ), and EXP1 ( Brummell et al , 1999 b ) were detected in B+4 fruits and quantified relative to expression in WT fruits ( Fig. 4A–I ).…”

“…PE is involved in pectin depolymerization and affects tissue integrity in over-ripe fruit ( Brummell and Harpster, 2001 ). Silencing PL at the mRNA level improved tomato shelf-life; this improvement was caused by differences in the levels of total pectin and soluble pectin ( Uluisik et al , 2016 ). MAN is involved in fruit shelf-life, without any negative effect on vegetative growth, fruit development, days to maturity, seed production, and yield ( Meli et al , 2010 ).…”

“…sugars, pigments, volatiles, and organic acids), increase shelf-life, and decrease spoilage rate. Recently, the suppression of cell wall modification-related genes has been used to reduce the softening of fruit in transgenic tomato ( Han et al , 2016 ; Uluisik et al , 2016 ; Yang et al , 2017 ). However, these studies have had very little success.…”

Primary Cell Wall Modifying Proteins Regulate Wall Mechanics to Steer Plant Morphogenesis

“…In addition to the pectin modification enzymes, the structure and composition of pectins are modulated by pectin depolymerases, including PELs and polygalacturonases (PGs). PELs, which degrade demethylesterified pectin, play crucial roles in leaf growth and senescence in rice ( Wu et al, 2013 ; Leng et al, 2017 ), pollen wall development in Chinese cabbage ( Jiang et al, 2014a , b ; Rhee et al, 2015 ), and fruit ripening and softening in tomato ( Uluisik et al, 2016 ; Yang et al, 2017 ; Wang et al, 2018 , 2019 ; Uluisik and Seymour, 2020 ). In rice, the mutation of a PEL precursor gene DWARF AND EARLY SENESCENCE LEAF1 ( DEL1 ) led to dwarfism and an early senescence leaf phenotype.…”

CRISPR technology is revolutionizing the improvement of tomato and other fruit crops

“…Hence, obtaining fruits that exhibit good shelf life without affecting other quality aspects is crucial. Two research groups have reported successful harnessing of fruit softening by silencing pectate lyase ( PL ) and alcobaca ( ALC ) without reducing tomato organoleptic and nutritional quality 108,109 , suggesting that the CRISPR system might be an excellent tool for fruit crop improvement.…”