Erratum: SPAG5 upregulation predicts poor prognosis in cervical cancer patients and alters sensitivity to taxol treatment via the mTOR signaling pathway

Yuan, L-J; Jd, Li; Zhang, L; Jh, Wang; Wan, Tingting; Zhou, Yanan; Tu, Heng; Yun, J-P; Luo, Rong; Jia, W-H; Zheng, Min

doi:10.1038/cddis.2015.163

Cited by 9 publications

(9 citation statements)

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“…Increased SPAG5 expression was reported to be accompanied by reduced survival in hepatocellular carcinoma [21]. SPAG5 upregulation was related to poor prognosis in cervical cancer patients [9]. SPAG5 expression in prostate cancer was associated with cancer progression and unfavorable outcomes [10].…”

Section: Discussionmentioning

confidence: 99%

SPAG5 upregulation contributes to enhanced c-MYC transcriptional activity via interaction with c-MYC binding protein in triple-negative breast cancer

Zhou

et al. 2019

J Hematol Oncol

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BackgroundTriple-negative breast cancer (TNBC) is an aggressive breast cancer subtype that lacks effective therapeutic targets. Sperm-associated antigen 5 (SPAG5) is a mitotic spindle-associated protein that is involved in various biological processes in cervical cancer and bladder urothelial carcinoma. However, the role of SPAG5 in TNBC remains undefined.MethodsThe expression of SPAG5 was examined in TNBC patients via quantitative real-time polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry (IHC). The biological functions of SPAG5 in TNBC and the underlying mechanisms were investigated in vitro and in vivo.ResultsSPAG5 expression was significantly upregulated in TNBC tissues compared with that in paired adjacent noncancerous tissues (ANTs). High SPAG5 expression was associated with increased lymph node metastasis and high risk of local recurrence. SPAG5 protein expression was significantly associated with poor disease-free survival in TNBC. Gene set enrichment analysis of TNBC data from The Cancer Genome Atlas (TCGA) indicated that high SPAG5 expression was significantly associated with cell cycle and the ATR-BRCA pathway. Functional assays demonstrated that SPAG5 expression promoted tumor growth in vitro and in vivo. In addition, SPAG5-silenced cells were more sensitive to the PARP inhibitor (PARPi) olaparib. Mechanistically, SPAG5 interacted with c-MYC binding protein (MYCBP), thereby increasing MYCBP protein levels and leading to increased c-MYC transcriptional activity, which promoted the expression of the c-MYC target genes: CDC20, CDC25C, BRCA1, BRCA2, and RAD51.Knockdown of MYCBP or c-MYC abolished the SPAG5-induced cell-cycle progression and cell proliferation of TNBC.ConclusionsCollectively, our results indict that SPAG5 is an efficient prognostic factor in TNBC, and that SPAG5 knockdown increases the sensitivity of TNBC to the PARPi olaparib. SPAG5 promotes tumor growth and DNA repair by increasing c-MYC transcriptional activity via interaction with MYCBP. The SPAG5/MYCBP/c-MYC axis may represent a potential therapeutic target for TNBC treatment.Electronic supplementary materialThe online version of this article (10.1186/s13045-019-0700-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

SPAG5 upregulation contributes to enhanced c-MYC transcriptional activity via interaction with c-MYC binding protein in triple-negative breast cancer

Zhou

et al. 2019

J Hematol Oncol

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“…All statistical analyses were performed using the SPSS 18.0 statistical software package (SPSS Inc., Chicago, IL, USA). For statistical analysis, the continuous SPAG5 expression data from IHC were first converted into dichotic data (low vs. high) using specific cutoff values, which were selected based on significant differences in OS using the X-tile software program () (14). Statistical analysis of the RT-qPCR data was performed using an unpaired Student's t-test when two groups were compared.…”

Section: Methodsmentioning

confidence: 99%

“…SPAG5 is involved in the formation of spindles during the cell cycle and may be closely associated with the function and movement of spindles during mitosis (12,13). Certain studies have also reported that SPAG5 may inhibit cell apoptosis through the inhibition of mammalian target of rapamycin complex 1 (14). In 2016, Abdel-Fatah et al (15) revealed that SPAG5 is an amplified gene in BC and may have clinical utility as a biomarker in estrogen receptor (ER)-negative BC, however this study did not examine the association between SPAG5 and the molecular classification of BC.…”

Section: Introductionmentioning

confidence: 99%

Sperm‑associated antigen 5 is a potential biomarker for poor prognosis in breast cancer

et al. 2018

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Sperm-associated antigen 5 (SPAG5) is currently considered to serve a role in promoting tumor cell growth and is overexpressed in several types of human cancer. However, to the best of our knowledge, the association of SPAG5 with molecular subtypes of patients with breast cancer (BC) remains to be fully investigated. Reverse transcription-quantitative polymerase chain reaction and immunohistochemistry on tissue microarrays were used in the current study to detect the expression levels of SPAG5 mRNA and protein, respectively, in BC. The association between SPAG5 mRNA and protein levels, and clinical characteristics and prognostic information were investigated. SPAG5 mRNA and protein levels were identified to be higher in BC tissues compared with matched adjacent nontumor tissues. High expression level of SPAG5 protein was associated with tumor size, histological grade, estrogen receptor expression, Ki-67 expression, lymph node status, tumor-node-metastasis (TNM) stage and the triple-negative BC subtype. In addition, high expression level of SPAG5 protein was associated with a poor prognosis in patients with BC. In summary, the current study suggests that SPAG5 is a novel and useful prognostic biomarker in BC.

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“…Western blotting was carried out according to the literature description ( 17 ). The primary antibodies are as follows: rabbit polyclonal SPAG5 (1:200, Sigma-Aldrich, Germany), mouse monoclonal GAPDH (1:2,000, Santa-Cruz, CA, USA), rabbit polyclonal CDH2 (1:100, Cell Signaling Technology, MA, USA), rabbit IgG (1:2,000, Santa-Cruz, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…During mitosis, SPAG5 could interact with many proteins, such as CLASP1, astrin, and Kif2b, to regulate the centromere–microtubule dynamics and thus promotes mitotic processes and their fidelity ( 10 ). It was reported that SPAG5 has participated in growth and progression of various tumors, which was overexpressed in breast cancer ( 11 , 12 ), osteosarcoma ( 13 ), lung cancer ( 14 ), bladder urothelial carcinoma ( 15 ), prostate cancer ( 16 ), and cervical cancer ( 17 ). Thus, it is deduced that SPAG5 may also take part in the tumorigenesis and progression of glioma.…”

Section: Introductionmentioning

confidence: 99%

SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

Wang

Cheng

et al. 2021

Front. Oncol.

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BackgroundGlioma is the most frequent malignant primary brain tumor in adults.ObjectiveTo explore the role of sperm-associated antigen 5 (SPAG5) in glioma.MethodsThe association between SPAG5 expression and clinical features was investigated based on The Cancer Genome Atlas (TCGA) datasets. The function of SPAG5 in glioma was analyzed using U87 and U251 cells. Knockdown glioma cells were constructed by shRNA interference. qRT-PCR and Western blotting were used to measure the expression of SPAG5 and Cadherin 2 (CDH2). Cell proliferation and apoptosis were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, caspase 3/7 assay, and high-content screening (HCS) proliferation analysis and colony formation assay. Transwell assays and wound-healing assays were used to investigate cell migration and invasion.ResultsThe increased expression of SPAG5 was correlated with poor outcomes in glioma patients. Knocking down SPAG5 could inhibit the proliferation and colony formation and promoted the apoptosis of glioma cells. Knocking down SPAG5 could also inhibit cell migration and invasion and the expression of CDH2. Overexpression of CDH2 with SPAG5 depletion could restore the proliferation and inhibit the apoptosis of glioma cells, which also promoted cell migration and invasion.ConclusionsSPAG5 is a promising prognostic factor and potential therapeutic target for clinical intervention in glioma.

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Erratum: SPAG5 upregulation predicts poor prognosis in cervical cancer patients and alters sensitivity to taxol treatment via the mTOR signaling pathway

Cited by 9 publications

References 0 publications

SPAG5 upregulation contributes to enhanced c-MYC transcriptional activity via interaction with c-MYC binding protein in triple-negative breast cancer

SPAG5 upregulation contributes to enhanced c-MYC transcriptional activity via interaction with c-MYC binding protein in triple-negative breast cancer

Sperm‑associated antigen 5 is a potential biomarker for poor prognosis in breast cancer

SPAG5 Is Involved in Human Gliomagenesis Through the Regulation of Cell Proliferation and Apoptosis

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