Erratum to: A reference human genome dataset of the BGISEQ-500 sequencer

Huang, Jie; Liang, Xinming; Xuan, Yuankai; Geng, Chunyu; Li, Yuxiang; Lu, Haorong; Qu, Su; Mei, Xianglin; Chen, Hongbo; Yu, Ting; Sun, Nan; Rao, Junhua; Wang, Jiahao; Zhang, Wenwei; Chen, Ying; Liao, Sha; Jiang, Hui; Liu, Xin; Yang, Zhaopeng; Mu, Feng; Gao, Shangxian

doi:10.1093/gigascience/giy144

Cited by 11 publications

(6 citation statements)

References 4 publications

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“…#A63882) according to the manual. All libraries were further prepared based on BGISEQ-500 sequencing platform with pair-end 50-bp read length (Huang et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

An ATAC-seq Dataset Uncovers the Regulatory Landscape During Axolotl Limb Regeneration

Wei

Guo³

et al. 2021

Front. Cell Dev. Biol.

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“…#A63882) according to the manual. All libraries were further prepared based on BGISEQ-500 sequencing platform with pair-end 50-bp read length (Huang et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%

An ATAC-seq Dataset Uncovers the Regulatory Landscape During Axolotl Limb Regeneration

Wei

Guo³

et al. 2021

Front. Cell Dev. Biol.

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“…Total RNA was extracted from the cell samples for use in RNA-seq analysis. The RNA samples were converted into individual cDNA libraries and generated using the BGISEQ-500 platform of the Beijing Genomics Institute (BGI) (Beijing, China) 22 , 23 . All of the generated raw sequencing reads were filtered to obtain clean reads before storing in the FASTQ format 24 .…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was extracted from the cell samples for use in RNAseq analysis. The RNA samples were converted into individual cDNA libraries and generated using the BGISEQ-500 platform of the Beijing Genomics Institute (BGI) (Beijing, China) 22,23 .…”

Section: Rna-seq Analysismentioning

confidence: 99%

Complement C3a activates osteoclasts by regulating the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma

et al. 2021

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Objective: Myeloma bone disease (MBD) is the most common complication of multiple myeloma (MM). Our previous study showed that the serum levels of C3/C4 in MM patients were significantly positively correlated with the severity of bone disease. However, the mechanism of C3a/C4a in osteoclasts MM patients remains unclear. Methods: The formation and function of osteoclasts were analyzed after adding C3a/C4a in vitro. RNA-seq analysis was used to screen the potential pathways affecting osteoclasts, and the results were verified by Western blot, qRT-PCR, and pathway inhibitors. Results: The osteoclast area per view induced by 1 μg/mL (mean ± SD: 50.828 ± 12.984%) and 10 μg/mL (53.663 ± 12.685%) of C3a was significantly increased compared to the control group (0 μg/mL) (34.635 ± 8.916%) (P < 0.001 and P < 0.001, respectively). The relative mRNA expressions of genes, OSCAR/TRAP/RANKL/cathepsin K, induced by 1 μg/mL (median: 5.041, 3.726, 1.638, and 4.752, respectively) and 10 μg/mL (median: 5.140, 3.702, 2.250, and 5.172, respectively) of C3a was significantly increased compared to the control group (median: 3.

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“…The polymerase chain reaction product from eight pooled libraries was heat-denatured and ligated into single-strand circular DNA. After DNB generation, libraries were then loaded on sequencing chip and sequenced with paired-end 50-bp reads (Huang et al, 2017 ).…”

Section: Methodsmentioning

confidence: 99%