Erratum to: Cuprizone Model as a Tool for Preclinical Studies of the Effi cacy of Multiple Sclerosis Diagnosis and Therapy

Abakumova, Tatiana O.; Kuzkina, Anastasia; Zharova, Mariia; Pozdeeva, D. A.; Gubskii, I L; Shepeleva, I. I.; Antonova, O. M.; Nukolova, N. V.; Кекелидзе, Зураб Ильич; Chekhonin, V. P.

doi:10.1007/s10517-015-2976-8

Cited by 7 publications

(2 citation statements)

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“…The therapeutic effects of triptolide (oral administration) was also found in the EAE model of SJL/J mice, involving increased expression levels of Hsp70 and stabilisation of the NF-kB/inhibitor-kappa-B alpha (IkBα) complex ( Kizelsztein et al, 2009 ). In addition to the EAE model, there is also a cuprizone induced toxic model that can be used to study MS, which is primarily used to study damage to oligodendrocytes and myelin sheaths during MS ( Abakumova et al, 2015 ). A recent study demonstrated that, in cuprizone induced toxic models, triptolide administered intraperitoneally improved behavioral deficits and attenuated neuroinflammation by inhibiting NF-kB activation and promoting intrinsic myelin repair ( Sanadgol et al, 2018 ).…”

Section: Effects Of Triptolide and Celastrol On Neurodegenerative Dis...mentioning

confidence: 99%

The therapeutic potential of triptolide and celastrol in neurological diseases

2022

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Neurological diseases are complex diseases affecting the brain and spinal cord, with numerous etiologies and pathogenesis not yet fully elucidated. Tripterygium wilfordii Hook. F. (TWHF) is a traditional Chinese medicine with a long history of medicinal use in China and is widely used to treat autoimmune and inflammatory diseases such as systemic lupus erythematosus and rheumatoid arthritis. With the rapid development of modern technology, the two main bioactive components of TWHF, triptolide and celastrol, have been found to have anti-inflammatory, immunosuppressive and anti-tumor effects and can be used in the treatment of a variety of diseases, including neurological diseases. In this paper, we summarize the preclinical studies of triptolide and celastrol in neurological diseases such as neurodegenerative diseases, brain and spinal cord injury, and epilepsy. In addition, we review the mechanisms of action of triptolide and celastrol in neurological diseases, their toxicity, related derivatives, and nanotechnology-based carrier system.

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Section: Effects Of Triptolide and Celastrol On Neurodegenerative Dis...mentioning

confidence: 99%

The therapeutic potential of triptolide and celastrol in neurological diseases

2022

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“…Therefore, they have become one of the most promising methods for the treatment of MS with affective disorders due to their unique biological characteristics and clinical research background. CPZ is a selective copper ion chelating agent [8], it targets and damages oligodendrocytes, inducing demyelination in the central nervous system and establishes the demyelination sheath animal model [9,10]. Some regard this model suited to modelling mental disorders [11].…”

Section: Introductionmentioning

confidence: 99%

Effects of Bone Marrow Mesenchymal Stem Cells on Myelin Repair and Emotional Changes of a Cuprizone-Induced Demyelination Model

Chu¹,

Chen²,

Chen³

2023

J. Integr. Neurosci.

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Background: Multiple sclerosis (MS) is an autoimmune disease for which bone marrow mesenchymal stem cells (BM-MSCs) have become one of the most promising tools for treatment. Cuprizone(CPZ) induces demyelination in the central nervous system and its use has established a demyelination sheath animal modelwhich is particularly suitable for studying the effects of BM-MSCs on the remyelination and mood improvement of a demyelinating model mice. Methods: 70 C57BL/6 male mice were selected and divided into 4 groups: the normal control (n = 20), chronic demyelination (n = 20), myelin repair (n = 15) and cell-treated groups (n = 15). Mice in the normal control group were given a normal diet; the chronic demyelination group mice were given a 0.2% CPZ mixed diet for 14 weeks, mice in the myelin repair and cell-treated groups mice were given a 0.2% CPZ diet for 12 weeks and normal diet for 2 weeks, while the cell-treated group mice were injected with BM-MSCs from the 13th week. The cuprizone-induced demyelination model was successfully established and BM-MSCs extracted, behavioural changes of the mice were detected by open field test, elevated plus maze test and tail suspension test, demyelination and repair of the corpus callosum and astrocyte changes were observed by immunofluorescence and electron microscopy and the concentrations of monoamine neurotransmitters and their metabolites detected by enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography-electrochemistry (HPLC-ECD). Results: Results suggest BM-MSCs were successfully extracted and cultured, and migrated to the demyelinating area of brain tissue after cell transplantation. Compared with the normal control group, the mice in the chronic demyelination group showed obvious anxiety and depression behaviours (p < 0.05); compared with the chronic demyelination group, the anxiety and depression behaviours of the cell-treated group mice were improved (p < 0.05); compared with the normal control group, the demyelination of the corpus callosum region of the chronic demyelination group mice was significant (p < 0.01), while the myelin sheath of the cell-treated and myelin repair groups was repaired when compared with the chronic demyelination group (p < 0.05), and the cell-treated group had a more significant effect than the myelin repair group (p < 0.05). Compared with the normal control group, the number of astrocytes in the corpus callosum of the chronic demyelination group mice was significantly increased (p < 0.01), and the expression of glial fibrillary acidic protein (GFAP) in the cell-treated group was lower than that in the chronic demyelination and myelin repair groups (p < 0.05); the serum concentrations of norepinephrine (NE), 5-hydroxytryptamine (5-HT) and 5-Hydroxyindole-3-acetic acid (5-HIAA) between the normal control and the chronic demyelination groups were significantly different (p < 0.05). Conclusions: The CPZ-induced model can be used as an experimental carrier for MS combined with anxiety and depression, and BM-MSC trans...

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