Erratum to: New Biocomposite Materials Based on Hydrocarbon-Oxidizing Microorganisms and Their Potential for Oil Products Degradation

Nikolaev, Yu. A.; Borzenkov, I. A.; Demkina, E. V.; Лойко, Н. Г.; Канапацкий, Т. А.; Perminova, Irina V.; Khreptugova, Anna N.; Grigor’eva, N. V.; Bliznets, I. V.; Manucharova, N. A.; Sorokin, V. V.; Kovalenko, M. A.; Эль-Регистан, Г. И.

doi:10.1134/s0026261722300142

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“…SHG-embedded cells of HOB of diverse taxonomic positions varied in the preservation of their viability, as was evident from their ability to form colonies on solid media. Thus, the CFU titer of P. aeruginosa after 12 months of storage was ~100% of the initial value, i.e., it was 100 times higher than in the control variant, the planktonic culture stored for 12 months [ 6 ]. The survival of SHG-immobilized A. serfertii , P. extremoaustralis and R. qingshengii was an order of magnitude higher than in the control (without the gel).…”

Section: Resultsmentioning

confidence: 99%

“…The dynamics of death/survival for SHG-immobilized bacteria were quite different from non-immobilized cells [ 6 , 14 ]. The titer of the HOB cells surviving for several months was several orders of magnitude higher than in the control planktonic cultures.…”

Section: Discussionmentioning

confidence: 99%

“…Remediation usually includes the application of hydrocarbon-oxidizing bacteria (HOB) [ 3 , 4 ]. In the case of oil contamination, large-scale bioremediation required the development of efficient HOB-based preparations, as well as addressing issues such as the long-term preservation of high viable HOB titer during storage, transportation, and the application of the preparations and their improved introduction into contaminated soil biotopes [ 4 , 5 , 6 ]. HOB storage in an immobilized state on the surface of sorbents or incorporated into polymer gels is a promising approach to these problems [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…We have previously shown that the immobilization of HOB cultures into the gels based on humates and (3-aminopropyl)triethoxysilane (APTES) resulted in long-term HOB survival (12 months and longer during storage at temperatures of ~20 °C [ 6 , 14 ]. The titer of surviving cells was up to 1000 times higher than in the control, and the ability of these preparations to oxidize hydrocarbons was higher compared to the control (planktonic cultures).…”

Section: Introductionmentioning

confidence: 99%

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Ways of Long-Term Survival of Hydrocarbon-Oxidizing Bacteria in a New Biocomposite Material—Silanol-Humate Gel

et al. 2023

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Immobilized bacterial cells are presently widely used in the development of bacterial preparations for the bioremediation of contaminated environmental objects. Oil hydrocarbons are among the most abundant pollutants. We have previously described a new biocomposite material containing hydrocarbon-oxidizing bacteria (HOB) embedded in silanol-humate gels (SHG) based on humates and aminopropyltriethoxysilane (APTES); high viable cell titer was maintained in this material for at least 12 months. The goal of the work was to describe the ways of long-term HOB survival in SHG and the relevant morphotypes using the techniques of microbiology, instrumental analytical chemistry and biochemistry, and electron microscopy. Bacteria surviving in SHG were characterized by: (1) capacity for rapid reactivation (growth and hydrocarbon oxidation) in fresh medium; (2) ability to synthesize surface-active compounds, which was not observed in the cultures stored without SHG); (3) elevated stress resistance (ability to grow at high Cu2+ and NaCl concentrations); (4) physiological heterogeneity of the populations, which contained the stationary hypometabolic cells, cystlike anabiotic dormant forms (DF), and ultrasmall cells; (5) occurrence of piles in many cells, which were probably used to exchange genetic material; (6) modification of the phase variants spectrum in the population growing after long-term storage in SHG; and (7) oxidation of ethanol and acetate by HOB populations stored in SHG. The combination of the physiological and cytomorphological properties of the cells surviving in SHG for long periods may indicate a new type of long-term bacterial survival, i.e., in a hypometabolic state.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%