Erroneous HbA1c measurements in the presence of β-thalassemia and common Chinese hemoglobin variants

Abstract

β-Thalassemia can cause errors in HbA1c determination using the Variant II Turbo system. HbE heterozygosity or HbE homozygosity also complicated HbA1c measurements. The Capillarys 2 Flex Piercing system detected all Hb variants and HbA1c in patients with β-thalassemia and could provide measurements with high accuracy.

New trends in the long and puzzling history of HbA1c

“…However, this does not mean that all issues related to this subject have been addressed. For example, Ji et al demonstrate here that β-thalassemia can still lead to misinterpreting HbA 1c results, and highlight the importance of the optimal characterization and knowledge of every method with respect to interferences [17]. Moreover, less common variants may induce unexpected interferences with different impacts on the methods, as recently demonstrated elsewhere by Little et al [18].…”

Evaluation on the separated effect of 13 hemoglobin variants by a new automatic Hba1c analyzer

“…However, Tosoh HLC‐723 G8 analyzer could not detect some rare hemoglobin variants such as Hb J‐Baltimore in HbA1c mode 4 . Ji et al showed that heterozygous Hb E or homozygous Hb E might affect the detection of HbA1c by HPLC depending upon the specific assay machine 5 . Therefore, in the detection of HbA1c, the recognition of hemoglobin variants is very necessary.…”

Evaluation of the Interference of Hemoglobin Variant J-Bangkok on Glycated Hemoglobin (HbA1c) Measurement by Five Different Methods

“…The abnormal β chain hemoglobin in southern areas are mainly HbE, Hb Newyork, and Hb J-Bangkok, the Hb variants prevalent in northern China include HbG Taipei and HbD Punjab. [20] Previous studies have shown that the presence of Hb variants such as HbS, HbD, or HbE interfered with HbA1c measurement results with some methods [21], but few studies have examined the potential interference from Hb J-Bangkok. Hb Newyork, and HbG Taipei on HbA1c measurements.…”