Erythrocyte Glucose Uptake Increase in Patients With Diabetes Mellitus

Shamansurova, Zulaykho; Turakulov, Ya. Kh.; Akbarov, Z. S.; Normukhamedova, N.F.; Akhmedova, Mukhabbat; Kim, A. A.

doi:10.1042/bst028a161a

Cited by 2 publications

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“…14 To date, little information is available on the possible involvement of sialidase in diabetic disease. 1,[15][16][17] This enzyme is present in the erythrocytes, as opposed to other cell types, only at the plasma membrane level together with different sialoderivatives. Recent studies have shown that the erythrocyte plasma membrane is characterized by the presence of 2 different forms of sialidase having optimal pH in the acidic and neutral range, respectively, and also differing both in terms of membrane anchoring modalities and interaction with endogenous substrates.…”

Section: Introductionmentioning

confidence: 99%

Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patients

Venerando

Fiorilli

Croci

et al. 2002

Blood

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The behavior of the 2 sialidase forms present in the erythrocyte membrane was investigated in 117 subjects with type 2 diabetes mellitus versus 95 healthy controls. A significant increase of the acidic form of sialidase, which is anchored to the membrane by a glycosylphosphatidylinositol bridge, was observed in erythrocyte resealed membranes. On the contrary, the neutral form of the enzyme, the only one capable of removing lipid-and protein-bound sialic acid from endogenous and exogenous sialoderivatives, was significantly reduced with a consequent increase of erythrocyte membrane total sialic acid content. Disease duration, therapy, glycemia, parameters of metabolic control, and presence of complications, except nephropathies, had no influence on the tested enzyme activities. Diabetic subjects showed a different erythrocyte age distribution, with an almost double proportion of young red cells and only one quarter of senescent ones compared with controls. In young erythrocytes, diabetic and control subjects had the same distribution of the 2 enzymes, while in senescent cells the acidic enzyme was increased 3.5-fold and the neutral form was reduced by half in the diabetic subjects. The increase of both acidic sialidase and total membranebound sialic acid, together with an overpresence of young red cells in diabetics, suggests that in this pathological condition there might be an altered aging process with a diminished expression of the neutral form of the enzyme and an increase of bound sialic acid. It has been suggested that the expression of the neutral enzyme requires some activation mechanism that is impaired in diabetes.

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Section: Introductionmentioning

confidence: 99%

Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patients

Venerando

Fiorilli

Croci

et al. 2002

Blood

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“…Контрольную группу составили 11 лиц без СД. У обследуемых лиц определяли уро-вень Нb А1с [4,5], активность сиалидазы эритроцитов [5,6], уро-вень нитритов и нитратов крови -стабильных метаболитов оксида азота по [7,8], фосфор крови и мембран по [5], актив-ность НКАТФазы по уровню неорганического фосфора в при-сутствии АТФ [1,3]. Полученные результаты были обработаны методами вариационной статистики с расчетом M±m и оцен-кой достоверности данных при p<0,05 по критерию Стьюдента и расчета корреляции [9].…”

Section: материалы и методыunclassified

“…Активность сиалидазы эритроцитов (АСЭ) при СД отра-жает степень структурно-функциональных нарушений на плазматической мембране эритроцитов [6,8,10]. АСЭ была существенно повышенной при СД1 (в 10 раз, p<0,01) и СД2 (в 9,17 раз, p<0,01), по сравнению с лицами без диабета и согласовалось с данными литературы и предыдущими ре-зультатами [6].…”

Section: результаты и обсуждениеunclassified

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Abnormal Na-K-ATPase activity in erythrocytes from patients with diabetes mellitus

Shamansurova¹,

А²,

Tsoi³

et al. 2009

Diabetes mellitus

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Aim. To measure Na-K-ATPase activity in erythrocytes from patients with diabetes mellitus (DM), elucidate its relationship with clinical manifestations of the disease and biochemical blood characteristics.Materials and methods. A total of 76 DM patients were enrolled in this study (39 with DM1 and 35 with DM2). Control group consisted of 11 subjects without DM. They were examined for HbA1c, nitrite and nitrate levels in blood and membranes, sialidase activity in erythrocytes and Na-KATPase activity measured from variations of inorganic phosphorus content in the presence of ATP. Results. Na-K-ATPase activity in MD patients was significantly lower than in controls (p<0.05). It was unrelated to the patients' age and sex but dropped insignificantly with MD duration. Na-K-ATPase activity depended on blood HbA1c and membrane phosphorus levels, enhanced sialidase activity in erythrocytes, nitrite and nitrate concentrations in plasma. Conclusion. Na-K-ATPase activity reflects the degree of metabolic disturbances and endothelial dysfunction in patients with DM.

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Erythrocyte Glucose Uptake Increase in Patients With Diabetes Mellitus

Cited by 2 publications

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Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patients

Acidic and neutral sialidase in the erythrocyte membrane of type 2 diabetic patients

Abnormal Na-K-ATPase activity in erythrocytes from patients with diabetes mellitus

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