Erythrocyte membrane in rats fed high erucic acid-containing mustard oil: Osmotic fragility, lipid composition, and (Na+, K+)- and (Ca2+, Mg2+)-ATPases

Vajreswari, Ayyalasomayajula; Rao, P. Srinivasa; Kaplay, Suresh S.; Tulpule, P. G.

doi:10.1016/0006-2944(83)90056-x

Cited by 34 publications

(11 citation statements)

References 29 publications

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“…This is in contrast with results from mammalian erythrocytes where only minor changes were detected in the membrane fatty acid pattern (Rao et al 1979;Vajreswari et al 1983;Benga et aL 1984) even after feeding animals for several generations (Ehrstr6m et al 1981). Concurrently, the basic features of the fatty acid composition are largely modified by the diet as regards either the molar percentage of saturated fatty acids or the average unsaturation index.…”

Section: Discussioncontrasting

confidence: 64%

“…An important factor in determining the stability of the erythrocytes membrane is that of its fatty acid composition, in particular the level of unsaturated fatty acids. The abundance of unsaturated fatty acids (Raz and Livne 1973;Shand and Noble 1981;Vajreswari et al 1983) was shown to be related to a greater extent of membrane expansion when cells are exposed to hypotonic solutions. This increase in membrane surface allows an increase in cellular critical volume which is positively correlated with the osmotic resistance (Anderson and Lovrien 1977) when the resulting hemolysis is studied as a function of salt concentration.…”

Section: Discussionmentioning

confidence: 98%

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The effect of dietary lipids on the trout erythrocyte membrane

Leray

Nonnotte

1986

Fish Physiol Biochem

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Rainbow trout were fed either a commercial diet or semi-purified diets containing 8% by weight of either cod liver oil, grape seed oil or hydrogenated coconut oil. Important changes in the fatty acid composition of erythrocyte membrane phospholipids were induced by these dietary fats. No changes were seen in the cholesterol/phospholipid ratio and in the cellular ATP level. Shape changes (crenation of cell margin and shrinkage) were only seen in trout fed hydrogenated coconut oil in connection with an accumulation of high amounts of (n-9) fatty acids including 20:3 (n-9) phospholipids. The compositional changes affect the stability of the erythrocyte membrane. An increased rate of osmotic hemolysis appeared to be associated with an increased unsaturated fatty acid content of the membranes.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 98%

The effect of dietary lipids on the trout erythrocyte membrane

Leray

Nonnotte

1986

Fish Physiol Biochem

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“…Na + ,K + -ATPase activity was assayed according to Vajreshwari et al [23] in a medium containing 20 mM Tris-HCl (pH 7.4), 140 mM NaCl, 14 mM KCl, 3 mM MgCl 2 , 3 mM ATP and 0.2 mM EDTA in a final volume of 0.5 ml, and additionally, ouabain was also included at 1 mM for the assay of ouabain-sensitive enzyme activity. Reaction was started by adding ATP to the preincubated reaction mixture containing suitable aliquot of the enzyme (50 -100 Ag protein) and incubated at 378C for 1 h. The reaction was stopped by adding 0.5 ml of 10% TCA and then centrifuged to remove protein precipitate, and the supernatant was used to estimate the released inorganic phosphate (Pi).…”

Section: Na + K + -Atpasementioning

confidence: 99%

Beneficial influence of dietary curcumin, capsaicin and garlic on erythrocyte integrity in high-fat fed rats

Kempaiah

Srinivasan

2006

The Journal of Nutritional Biochemistry

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“…In this respect it is remarkable to notice how little variations are found in the overall phospl~lipid composition, and the fatty acid composition, under a variety of conditions. During the whole 120 days lifespan of the human erythrocyte, the overall phospholipid composition remains rather similar [1,9] and the differences in fatty acid composition which can be observed upon extreme variations in dietary fats are less pronounced than the changes in lipid composition of the plasma [10][11][12][13]. Relevant in this respect is the slow turnover of the membrane phospholipids: renewal of PC occurs by deacylation-reacylation as well as by an exchange of complete molecules with the serum; both processes proceed with a rate of about 1% per hour [14] and a halftime of 100 h [15], respectively.…”

Section: Introductionmentioning

confidence: 99%

The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

Kuypers

Roelofsen

Kamp

et al. 1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

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(1) Using the phosphatidylcholine specific transfer protein from bovine liver, native phosphatidylcholine from intact human erythrocytes was replaced by a variety of different phosphatidylcholine species without altering the original phospholipid and cholesterol content. (2) The replacement of native phospharidylcholine by the disaturated species, 1,2-dipalmitoyl-and 1,2-distearoylphosphatidyicholine, proceeded at a low rate and extensive replacement could only be achieved by repeatedly adding fresh donor vesicles. The replacement by disaturated molecules was accompanied by a gradual increase in osmotic fragility of the cells, finally resulting in hemolysis when 40% of the native PC had been replaced. Up to this lyric concentration, the replacement did not affect the permeability of the membrane for potassium ions. (3) Essentially, all of the PC in the outer monolayer of the membrane could be replaced by 1-palmitoyl-2-oleoyl-and 1-palmitoyl-2-1ino-leoylphosphatidylcholine. These replacements did not alter the osmotic fragility of the cells, nor the K + permeability of the membrane. (4) Increasing the total degree of unsaturation of the phosphatidyicholine species modified the properties of the membrane considerably. Replacement by 1,2-dilinoleoylphosphatidylcholine resulted in a progressive increase in osmotic fragility and hemolysis started to occur after 30% of the native PC had been replaced by this species. K ÷ permeability was found to be slightly increased in this case. Cells became leaky for K ÷ upon the introduction of 1-palmitoyl-2-arachidonoylphosphatidyicholine in the membrane. The increased permeability was also reflected by an apparent increase in the resistance of the cells against osmotic shock. (5) The conclusions to be drawn are that (i) 1-palmitoyl-2-oleoyi-and l-paimitoyi-2-1inoleoylphosphatidyicholine are species which fit most optimally into the erythrocyte membrane; (ii) loss of membrane stability results from an increase in the degree of saturation of phosphatidyicholine (unsaturation index > 0.5) and (iii) the permeability is enhanced by increasing the content of highly unsaturated species (unsaturation index > 1.0).

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Erythrocyte membrane in rats fed high erucic acid-containing mustard oil: Osmotic fragility, lipid composition, and (Na+, K+)- and (Ca2+, Mg2+)-ATPases

Cited by 34 publications

References 29 publications

The effect of dietary lipids on the trout erythrocyte membrane

The effect of dietary lipids on the trout erythrocyte membrane

Beneficial influence of dietary curcumin, capsaicin and garlic on erythrocyte integrity in high-fat fed rats

The membrane of intact human erythrocytes tolerates only limited changes in the fatty acid composition of its phosphatidylcholine

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