Erythrocytes as model cells for biocompatibility assessment, cytotoxicity screening of xenobiotics and drug delivery

Podsiedlik, Maria; Markowicz-Piasecka, Magdalena; Sikora, Joanna

doi:10.1016/j.cbi.2020.109305

Cited by 55 publications

(30 citation statements)

References 178 publications

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“…The skeleton of the erythrocyte exhibits specific characteristics, such as the absence of the structural protein tubulin. The role of the skeleton of the erythrocyte is mainly devoted to the shape of the cell and its capacity for deformability [ 26 ]. Specific proteins in the cytoskeleton of the erythrocyte, such as spectrin, protein 4.1, F-actin and ankyrin, play determinant roles in the regulation of cell deformability.…”

Section: Erythrocytes—simple Vital and Fragile Cellsmentioning

confidence: 99%

Erythrocytes: Central Actors in Multiple Scenes of Atherosclerosis

Turpin

Catan

Meilhac

et al. 2021

IJMS

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The development and progression of atherosclerosis (ATH) involves lipid accumulation, oxidative stress and both vascular and blood cell dysfunction. Erythrocytes, the main circulating cells in the body, exert determinant roles in the gas transport between tissues. Erythrocytes have long been considered as simple bystanders in cardiovascular diseases, including ATH. This review highlights recent knowledge concerning the role of erythrocytes being more than just passive gas carriers, as potent contributors to atherosclerotic plaque progression. Erythrocyte physiology and ATH pathology is first described. Then, a specific chapter delineates the numerous links between erythrocytes and atherogenesis. In particular, we discuss the impact of extravasated erythrocytes in plaque iron homeostasis with potential pathological consequences. Hyperglycaemia is recognised as a significant aggravating contributor to the development of ATH. Then, a special focus is made on glycoxidative modifications of erythrocytes and their role in ATH. This chapter includes recent data proposing glycoxidised erythrocytes as putative contributors to enhanced atherothrombosis in diabetic patients.

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Section: Erythrocytes—simple Vital and Fragile Cellsmentioning

confidence: 99%

Erythrocytes: Central Actors in Multiple Scenes of Atherosclerosis

Turpin

Catan

Meilhac

et al. 2021

IJMS

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“…This eventually leads to the formation of holes in the membrane and ultimately hemolysis. The use of the free radical reactions initiator AAPH is recommended as more appropriate to measure radical-scavenging activity in vitro as the activity of peroxyl radicals shows a greater similarity to cellular activities such as lipid peroxidation [ 49 ]. Based on the nature of the radicals and the cell model used, this method is biologically relevant and appropriate for estimating the antioxidant activity of novel compounds.…”

Section: Resultsmentioning

confidence: 99%

Insights into the Antioxidant Mechanism of Newly Synthesized Benzoxazinic Nitrones: In Vitro and In Silico Studies with DPPH Model Radical

et al. 2021

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Synthetic nitrone spin-traps are being explored as therapeutic agents for the treatment of a wide range of oxidative stress-related pathologies, including but not limited to stroke, cancer, cardiovascular, and neurodegenerative diseases. In this context, increasing efforts are currently being made to the design and synthesis of new nitrone-based compounds with enhanced efficacy. The most researched nitrones are surely the ones related to α-phenyl-tert-butylnitrone (PBN) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) derivatives, which have shown to possess potent biological activity in many experimental animal models. However, more recently, nitrones with a benzoxazinic structure (3-aryl-2H-benzo[1,4]oxazin-N-oxides) have been demonstrated to have superior antioxidant activity compared to PBN. In this study, two new benzoxazinic nitrones bearing an electron-withdrawing methoxycarbonyl group on the benzo moiety (in para and meta positions respect to the nitronyl function) were synthesized. Their in vitro antioxidant activity was evaluated by two cellular-based assays (inhibition of AAPH-induced human erythrocyte hemolysis and cell death in human retinal pigmented epithelium (ARPE-19) cells) and a chemical approach by means of the α,α-diphenyl-β-picrylhydrazyl (DPPH) scavenging assay, using both electron paramagnetic resonance (EPR) spectroscopy and UV spectrophotometry. A computational approach was also used to investigate their potential primary mechanism of antioxidant action, as well as to rationalize the effect of functionalization on the nitrones reactivity toward DPPH, chosen as model radical in this study. Further insights were also gathered by exploring the nitrone electrochemical properties via cyclic voltammetry and by studying their kinetic behavior by means of EPR spectroscopy. Results showed that the introduction of an electron-withdrawing group in the phenyl moiety in the para position significantly increased the antioxidant capacity of benzoxazinic nitrones both in cell and cell-free systems. From the mechanistic point of view, the calculated results closely matched the experimental findings, strongly suggesting that the H-atom transfer (HAT) is likely to be the primary mechanism in the DPPH quenching.

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“…Erythrocytes are a simple imitation of the endosomal membrane, therefore are a model to study protein-membrane interactions and in vitro biocompatibility assessment of vital biotherapeutics [ 68 , 69 ]. Intravenously administered therapeutic agent can trigger hemolysis which is characterized by the release of hemoglobin into the plasma, induced by disruption, perturbation, or breakdown of the red blood cell membrane [ 70 , 71 ], making research into specific proteins as carrier molecules imperative [ 72 , 73 ].…”

Section: Resultsmentioning

confidence: 99%

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

Nubi

Adewole²,

Agunbiade

et al. 2021

Biotechnology Reports

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Highlights Purification of Moringa oleifera seed lectin (MoL) was achieved by ammonium sulfate precipitation and size exclusion chromatography MoL hemagglutinating activity was thermostable up to 90°C, within pH 2-4 and moderately inhibited by sucrose and fructose Traces of Ca2+, Mg2+, and Na+ was detected in MoL, and modification of arginine and tryptophan residues affected the hemagglutinating activity Hemolytic (membrane-perturbing) activity of MoL was potentiated in mildly acidic pH, and dependent on concentration, temperature, pH, and time of incubation.

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Erythrocytes as model cells for biocompatibility assessment, cytotoxicity screening of xenobiotics and drug delivery

Cited by 55 publications

References 178 publications

Erythrocytes: Central Actors in Multiple Scenes of Atherosclerosis

Erythrocytes: Central Actors in Multiple Scenes of Atherosclerosis

Insights into the Antioxidant Mechanism of Newly Synthesized Benzoxazinic Nitrones: In Vitro and In Silico Studies with DPPH Model Radical

Purification and erythrocyte-membrane perturbing activity of a ketose-specific lectin from Moringa oleifera seeds

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