Erythropoietic kinetics in sheep studied by means of induced changes in hemoglobin phenotype

Gabuzda, Thomas G.; Schuman, Marc A.; Silver, Ruth; Lewis, Hugh B.

doi:10.1172/jci105880

Cited by 42 publications

(13 citation statements)

References 24 publications

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“…The period of 5 to 7 days between the appearance of ESF activity in sheep 6019 and the first appearance of Hb-C corresponds to the time of production of Hb-C after injections of purified erythropoietin (Huisman et al, 1969) and after administration of anaemic plasma (Boyer, 1967;Cabuzda et al, 1968).…”

Section: Discussionmentioning

confidence: 99%

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The Production of Haemoglobin C in Adult Sheep and Goats

1969

Aust J Exp Biol Med

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Summary. Tbe production of baemoRlobin C (Hb-C) wa.s examined in tbree sheep and two goats wbicb were kept anaemic by bleeding for 5-7 weeks. A fourtb sbeep was made acutely anaemic Ijy bleeding severely for tbree days.In the chronically bled sheep the production of Hb-C wa.s variable and difficult to maintain unless the animals were made severely anaemic.In tbe goats tbe replacement of the ^ chain was almost complete witb an anaemia wbicb was equivalent to tbat produced in tbe sbeep. and Hb-C could be maintained for a considerable period.In the acutely bled sbeep Hb-C increased to a maximum by tbe 16th day, ReticulocytosLS was not marked, but, in general, an increase occurred about 15 days before maximum Hb-C production was evident.Erytbropoietin activity was examined in four animals witb variable results, bnt in the severely bled animal it was evident before tbe reticulocytes appeared. Tbis activity did not appear to he related to tbe amount of Hb-C whicii was produced.The elimination of Hb-C from tbe (irculation was very variable, but tbe rates of decrease in goats were slower than in sbeep.INTRODUCTION.

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Section: Discussionmentioning

confidence: 99%

“…It has been shown that the synthesis of this chain can be increased by injection of erythropoietin-rich material {Moore, Codley, van Vliet, Levids, Boyd and Huisman, 1966;Huisman et aL, 1969), and of plasma from sheep made anaemic with phenylhydrazine {Boyer, 1967; Gabuzda, Schuman, Silver and Lewis, 1968).…”

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confidence: 99%

The Production of Haemoglobin C in Adult Sheep and Goats

1969

Aust J Exp Biol Med

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“…This switch occurs because of a change in beta-chain synthesis; the type of alpha chain synthesized remains constant. Gabuzda et al (9) have shown that the anemia-induced switch from OA to tic globin in sheep is characterized by a 3-day lag between the onset of anemia and the beginning of gc production, implying that the switch occurs early in erythropoiesis, perhaps at the erythroid stem-cell stage. Enhanced production of HbC in both nonanemic sheep and goats by injection of erythropoietin suggests that the switch may be mediated by this hormone (7,10).…”

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confidence: 99%

“…Subsequent steps were performed exactly as described above for the preparation of mRNA from bone marrow cells. 9 mM, (2) 17 inM, (3) 27 mM, (4) 27 mM, and (5) 60 mM. Flow rate was 0.3-0.4 ml/min.…”

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confidence: 99%

Hemoglobin Switching in Sheep and Goats: Change in Functional Globin Messenger RNA in Reticulocytes and Bone Marrow Cells

Nienhuis

Anderson

1972

Proc. Natl. Acad. Sci. U.S.A.

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“…As the animal matures, hemoglobin C synthesis ceases and is replaced by the adult hemoglobin; hemoglobin A (c~d32 A) in sheep and hemoglobin A, D, or E (containing flA, /3D, and /3 ~, respectively) in goats. In bone marrow cells of adult animals, hemoglobin C production can be reinitiated in vivo by anemic stress or injection of ESF (7,10,23) and in vitro by exposure to ESF (1,3,4).…”

mentioning

confidence: 99%