This chapter discusses the effects of template ageing in EEG-based biometrics. The chapter also serves as an introduction to general biometrics and its main tasks: Identification and verification. To do so, we investigate different characterisations of EEG signals and examine the difference of performance in subject identification between single session and cross-session identification experiments. In order to do this, EEG signals are characterised with common state-of-the-art features, i.e. Mel Frequency Cepstral Coefficients (MFCC), Autoregression Coefficients, and Power Spectral Density-derived features. The samples were later classified using various classifiers, including Support Vector Machines and k-Nearest Neighbours with different parametrisations. Results show that performance tends to be worse for crosssession identification compared to single session identification. This finding suggests that temporal permanence of EEG signals is limited and thus more sophisticated methods are needed in order to characterise EEG signals for the task of subject identification.