ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias

Ishibashi, Tatsuro; Yokota, Takafumi; Tanaka, Hirokazu; Ichii, Michiko; Sudo, Takao; Satoh, Yusuke; Doi, Yukiko; Ueda, Tomoaki; Tanimura, Akira; Hamanaka, Yuri; Ezoe, Sachiko; Shibayama, Hirohiko; Oritani, Kenji

doi:10.1016/j.exphem.2015.12.010

Cited by 28 publications

(27 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As recently reported 39 , EPCR/PAR1 signaling facilitated LT-HSC BM repopulation, retention and the survival. Very recently, Yokota et al reported that ESAM was a useful purification marker for human BM and CB-derived HSCs 40 . As expected from our ultra-high-resolution purification method, CD34 + and CD34 − SRCs (HSCs) expressed higher levels of PROM1 (CD133) than non-SRCs.…”

Section: Resultsmentioning

confidence: 99%

A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells

et al. 2018

View full text Add to dashboard Cite

We previously identified CD34-negative (CD34−) severe combined immunodeficiency (SCID)-repopulating cells as primitive hematopoietic stem cells (HSCs) in human cord blood. In this study, we develop a prospective ultra-high-resolution purification method by applying two positive markers, CD133 and GPI-80. Using this method, we succeed in purifying single long-term repopulating CD34− HSCs with self-renewing capability residing at the apex of the human HSC hierarchy from cord blood, as evidenced by a single-cell-initiated serial transplantation analysis. The gene expression profiles of individual CD34+ and CD34− HSCs and a global gene expression analysis demonstrate the unique molecular signature of CD34− HSCs. We find that the purified CD34− HSCs show a potent megakaryocyte/erythrocyte differentiation potential in vitro and in vivo. Megakaryocyte/erythrocyte progenitors may thus be generated directly via a bypass route from the CD34− HSCs. Based on these data, we propose a revised road map for the commitment of human CD34− HSCs in cord blood.

show abstract

Section: Resultsmentioning

confidence: 99%

A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Moreover, it was shown that ESAM hi HSC of 5‐FU treated BM can reconstitute long‐term hematopoiesis, suggesting that ESAM hi HSC retain their engrafting capacity even after entering cell cycle [Sudo et al, ]. The functional importance of ESAM for HSC reconstituting activity was emphasized by the finding that ESAM‐KO mice suffer from severe BM suppression after 5‐FU administration due to a delayed erythroid recovery [Ishibashi et al, ].…”

Section: Soluble Factors and Surface Proteinsmentioning

confidence: 99%

Niche Extracellular Matrix Components and Their Influence on HSC

et al. 2017

View full text Add to dashboard Cite

Maintenance of hematopoietic stem cells (HSC) takes place in a highly specialized microenvironment within the bone marrow. Technological improvements, especially in the field of in vivo imaging, have helped unravel the complexity of the niche microenvironment and have completely changed the classical concept from what was previously believed to be a static supportive platform, to a dynamic microenvironment tightly regulating HSC homeostasis through the complex interplay between diverse cell types, secreted factors, extracellular matrix molecules, and the expression of different transmembrane receptors. To add to the complexity, non-protein based metabolites have also been recognized as a component of the bone marrow niche. The objective of this review is to discuss the current understanding on how the different extracellular matrix components of the niche regulate HSC fate, both during embryonic development and in adulthood. Special attention will be provided to the description of non-protein metabolites, such as lipids and metal ions, which contribute to the regulation of HSC behavior. J. Cell. Biochem. 118: 1984-1993, 2017. © 2017 Wiley Periodicals, Inc.

show abstract

“…To validate additional targets using non-mass spectrometry techniques, we performed FACS analysis and fluorescence microscopy. The endothelial surface adhesion molecule (ESAM) has previously been shown to be highly expressed by HSCs (Ishibashi et al, 2016;Ooi et al, 2009;Yokota et al, 2009) proteomic expression data further support a glycolytic advantage in the HSC compartment (Ito and Suda, 2014).…”

Section: Validation Of Differential Expression Of Proteins Of Interestmentioning

confidence: 86%

Mass spectrometry analysis of mouse hematopoietic stem cells and their progenitors reveals differential expression within and between proteome and transcriptome throughout adult and aged hematopoiesis

Zaro

Noh

Mascetti

et al. 2019

Preprint

View full text Add to dashboard Cite

Hematopoietic stem cells (HSCs) are responsible for the generation of blood and immune cells throughout life.They have the unique ability to self-renew and generate more HSCs or differentiate into a progenitor cell in response to cell-intrinsic and -extrinsic stimuli. The balance of HSC fate commitment is critical for a healthy blood supply. Imbalances during hematopoiesis, which are frequent in aging, can result in hematological malignancies and pre-malignancies as well as increase risk of atherosclerosis. Given the importance of HSCs and their progenitors, they have been extensively characterized in genomic and transcriptomic studies.However, an understanding of protein expression within the HSC compartment and more broadly throughout hematopoiesis remains poorly understood, and it has been widely reported that the correlation between mRNA and proteins is more complicated than previously thought. Previous mouse mass spectrometry studies have focused either specifically on stem and the first early progenitor or broadly across mixed populations of stem and progenitor cells, which do not allow for cell-type specific protein resolution across stages of differentiation.Mass cytometry has been employed to characterize transcription factor expression in human HSCs and progenitors but does not apply an unbiased discovery approach. New mass spectrometry technology now allows for deep proteomic coverage with no more than 200 ng of sample input. We report here a proteomics resource characterizing protein expression in mouse adult and aged HSCs, multipotent progenitors and olig progenitors, 12 cell types in total. We validated differential expression by flow cytometry analys immunofluorescence staining. Additionally, we investigated the relationship between mRNA and protein of individual genes in HSCs compared to progenitors through RNA sequencing studies and identifi proteins that appear to be uniquely regulated in the HSC compartment, Cpin1 and Adnp. In summa resource provides proteomic coverage of adult and aged hematopoietic stem cells and their progenito reveals changes in protein abundance between cell types, with potential future implications in unders mechanisms for stem-cell maintenance, niche interactions and fate determination.

show abstract

ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias

Cited by 28 publications

References 40 publications

A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells

A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells

Niche Extracellular Matrix Components and Their Influence on HSC

Mass spectrometry analysis of mouse hematopoietic stem cells and their progenitors reveals differential expression within and between proteome and transcriptome throughout adult and aged hematopoiesis

Contact Info

Product

Resources

About