ESBL-producing Shiga-toxigenic E. coli (STEC) associated with piglet diarrhoea in India

Mandakini, Rajkumari; Dutta, Tapan Kumar; Chingtham, Santhalembi; Roychoudhury, P.; Samanta, Indranil; Joardar, S. N.; Pachauau, Abigail R.; Chandra, Rajesh

doi:10.1007/s11250-014-0731-1

Cited by 23 publications

(17 citation statements)

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“…eSBL-producing E. coli belonging to an untypeable serogroup was isolated earlier from piglets in India (MaNDakINI et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…the birds are successfully reared in a backyard system because they can easily scavenge food like indigenous birds (ahUJa et al, 2008). In India, earlier studies conducted P. Ghosh et al: extended-spectrum cephalosporinase possessing E. coli in kuroilers in healthy livestock, such as cattle, pigs and broilers, revealed the existence of eSBLproducing E. coli and Klebsiella pneumoniae (LaLZaMPUIa et al, 2013;LaLZaMPUIa et al, 2014;SaMaNta et al, 2015;SaMaNta et al, 2015a;kar et al, 2015;MaNDakINI et al, 2015). the status of other domestic birds such as kuroilers as reservoirs of extendedspectrum cephalosporinase-producing E. coli is still unexplored.…”

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confidence: 99%

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Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

et al. 2017

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the present study was undertaken to detect the incidence of extended-spectrum-cephalosporinase gene possessing-Escherichia coli, its co-resistance pattern against other antimicrobials, and the clonal relationship of the isolates in healthy kuroiler birds. a total of 80 cloacal swabs from kuroilers were collected randomly from West Bengal, India. the use of costly antimicrobials (cephalosporins) was not practiced by farmers. Escherichia coli was isolated and identified by standard biochemical tests and 16SrRNA-PCR. All the E. coli isolates, including controls, were subjected to PCr for detection of bla CTX-M , bla TEM, bla SHV, and bla CMY-2 genes. By comparing the raPD-banding pattern, the phylogenetic relationship among the isolates was established. all the isolates were tested for phenotypical resistance against other antibiotics. In total, 60 isolates were identified as E. coli from the kuroilers studied (n = 80). among them, 12 (20%) isolates possessed one of the studied extended-spectrum cephalosporinase genes. among the studied genes, bla TEM and bla SHV were detected in 6 (10%) and 12 (20%) E. coli isolates, respectively. None of the E. coli isolates possessed bla CTX-M and bla In phylogenetic analysis, the strains isolated from same localities with similar genetic profile were grouped into the same cluster. resistance of extended-spectrum cephalosporinase gene possessing E. coli isolates was observed most frequently against ampicillin/cloxacillin, co-trimoxazole, amoxyclav, piperacillin, ceftriaxone, and tetracycline. Kuroiler birds with no cephalosporin usage profile may act as a reservoir of extended-spectrum cephalosporinase gene possessing E. coli. This is the first systematic study in kuroilers, to raise the awareness of consumers regarding the possibility of transmission of antimicrobial resistant E. coli from them.

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“…eSBL-producing E. coli belonging to an untypeable serogroup was isolated earlier from piglets in India (MaNDakINI et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

et al. 2017

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“…Among the serogroups isolated, untypeable was detected with maximum frequency (4/12, 33.3%) as ESBL/beta-lactamase producers. Similarly Mandakini et al (2015) reported the highest frequency of UT (11/43, 25.5%) followed by O26 (8/ 43, 18.6%) in ESBL-E. coli isolated from diarrhoeic pigs in India.…”

Section: Isolate Nomentioning

confidence: 91%

Approaches to characterize extended spectrum beta-lactamase/beta-lactamase producing Escherichia coli in healthy organized vis-a-vis backyard farmed pigs in India

Samanta

Joardar

Mahanti

et al. 2015

Infection, Genetics and Evolution

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“…All the suspected ESBLs producing isolates were subjected to PCR for detection of ESBLs producing genes ( bla TEM , bla SHV , bla CTX-M , and bla CMY ) using specific primers [ 5 ]. PCR was carried out in a 0.2 ml thin wall PCR tubes with a final volume of 25 µl containing 10× buffer, 1.5 mM MgCl 2 , 200 pM of each oligonucleotide primers, 200 µM of each dNTPs, 1 U of Taq polymerase, and 50 pg DNA.…”

Section: Methodsmentioning

confidence: 99%

“…Although a handful of sporadic report on MDR enteric bacteria of animal origin from different parts of India is available, so far, very little is known about the MDR bacteria in pigs of Northeastern region of India [ 5 ]. The present study was undertaken to record the prevalence of ESBLs producing E. coli , Salmonella spp., and K. pneumoniae from pig population of Assam and Meghalaya and to record the ability of the resistant bacteria to transfer the resistance genes horizontally.…”

Section: Introductionmentioning

confidence: 99%

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

Lalruatdiki¹,

Dutta²,

Roychoudhury³

et al. 2018

Vet World

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Aim:The present study was conducted to record the prevalence of extended spectrum β-lactamases (ESBLs) producing Escherichia coli, Salmonella spp., and Klebsiella pneumoniae from pig population of Assam and Meghalaya and to record the ability of the resistant bacteria to transfer the resistance genes horizontally.Materials and Methods:Fecal samples (n=228), collected from pigs of Assam (n=99) and Meghalaya (n=129), were processed for isolation and identification of E. coli and Salmonella spp. All the isolates were tested for ESBLs production by double disc synergy test (DDST) followed by screening for ESBLs producing genes (blaTEM, blaSHV, blaCTX-M, and blaCMY) by polymerase chain reaction (PCR). Possible transfer of resistance encoding genes between enteric bacterial species was carried out by in vitro and in vivo horizontal gene transfer (HGT) method.Results:A total of 897 enteric bacteria (867 E. coli and 30 Salmonella) were isolated and identified. Altogether 25.41% isolates were confirmed as ESBL producers by DDST method. Majority of the isolates were E. coli followed by Salmonella. By PCR, 9.03% isolates were found positive for at least one of the target resistance genes. blaSHV was absent in all the isolates. blaCMY was the most prevalent gene. All the E. coli isolates from Assam were negative for blaTEM. A total of 2.76% isolates were positive for blaTEM + blaCMY. On the other hand, 0.67% isolates were positive for blaCTX-M + blaCMY genes. Only 0.33% isolates carried all the three genes. Altogether, 4.68% bacteria carried the resistance encoding genes in their plasmids. blaTEM gene could be successfully transferred from Salmonella (donor) to E. coli (recipient) by in vitro (5.5-5.7×10−5) and in vivo (6.5×10−5 to 8.8×10−4) methods. In vivo method was more effective than in vitro in the transfer of resistance genes.Conclusion:The pig population of Assam and Meghalaya are carrying multidrug resistance and ESBLs producing E. coli and Salmonella. The isolates are also capable to transfer their resistance trait to other bacterial species by HGT. The present finding could be considered as a serious public health concern as similar trait can also be transmitted to the human commensal bacteria as well as pathogens.

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ESBL-producing Shiga-toxigenic E. coli (STEC) associated with piglet diarrhoea in India

Cited by 23 publications

References 23 publications

Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

Approaches to characterize extended spectrum beta-lactamase/beta-lactamase producing Escherichia coli in healthy organized vis-a-vis backyard farmed pigs in India

Extended-spectrum β-lactamases producing multidrug resistance Escherichia coli, Salmonella and Klebsiella pneumoniae in pig population of Assam and Meghalaya, India

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