Escherichia coli as a Multifaceted Pathogenic and Versatile Bacterium

Braz, Vânia Santos; Melchior, Karine; Moreira, Cristiano G.

doi:10.3389/fcimb.2020.548492

Cited by 117 publications

(94 citation statements)

References 111 publications

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“…It is well-known that LL-37 has antimicrobial activity against many Gram-positive and Gram-negative bacteria. We selected Staphylococcus aureus and Escherichia coli to test the bactericidal activity of SmbP_LL-37 and free LL-37, as they are some of the most common pathogenic bacteria [25,26]. As shown in Figure 6, the complete SmbP_LL-37 protein showed antimicrobial activity against both bacterial species: E. coli (ATCC 25922) and S. aureus (ATCC 25923).…”

Section: Resultsmentioning

confidence: 99%

The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37

et al. 2021

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(1) Background: The cathelicidin peptide LL-37 is a prominent molecule with many biological activities, including antimicrobial. Due to its importance, here, we describe the production of LL-37 tagged with SmbP, a relatively new carrier protein that improves the production of recombinant proteins and peptides in Escherichia coli. We present an alternative method for the rapid expression, purification, and antimicrobial evaluation of LL-37, that involves only one purification step. (2) Methods: A DNA construct of SmbP_LL-37 was transformed into E. coli BL21(DE3); after overnight expression, the protein was purified directly from the cell lysate using immobilized metal-affinity chromatography. SmbP_LL-37 was treated with Enterokinase to obtain the free LL-37 peptide. The antimicrobial activity of both SmbP_LL-37 and free LL-37 was determined using the colony forming unit assay method. (3) Results: SmbP_LL-37 was observed in the soluble fraction of the cell lysate; after purification with IMAC, protein gel electrophoresis, and analysis by ImageJ, it showed 90% purity. A total of 3.6 mg of SmbP_LL-37 was produced from one liter of cell culture. SmbP_LL-37 and free LL-37 both showed inhibition activity against Staphylococcus aureus and Escherichia coli. (4) Conclusions: The SmbP fusion protein is a valuable tool for producing biologically-active LL-37 peptide. The production method described here should be of interest for the expression and purification of additional cationic peptides, since it cuts the purification time considerably prior to determination of antimicrobial activity.

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Section: Resultsmentioning

confidence: 99%

The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37

et al. 2021

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“…It can be inferred that genes harbored by the ISEcp1 positive strains are more likely to be widely disseminated, as this genetic platform has been associated with the mobilization and improved expression of bla CTX-M [80,81]. In general, mobile genetic elements have contributed to the emergence of novel E. coli hybrid strains with distinct assortment of antimicrobial resistance traits [82].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

et al. 2021

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The prevalence of multidrug resistant, extended spectrum β-lactamase (ESBL)-producing Enterobacteriaceae is increasing worldwide. The present study aimed to provide an overview of the multidrug resistance phenotype and genotype of ESBL-producing Escherichia coli (E. coli) isolates of livestock and wild bird origin in Greece. Nineteen phenotypically confirmed ESBL-producing E. coli strains isolated from fecal samples of cattle (n = 7), pigs (n = 11) and a Eurasian magpie that presented resistance to at least one class of non β-lactam antibiotics, were selected and genotypically characterized. A DNA-microarray based assay was used, which allows the detection of various genes associated with antimicrobial resistance. All isolates harbored blaCTX-M-1/15, while blaTEM was co-detected in 13 of them. The AmpC gene blaMIR was additionally detected in one strain. Resistance genes were also reported for aminoglycosides in all 19 isolates, for quinolones in 6, for sulfonamides in 17, for trimethoprim in 14, and for macrolides in 8. The intI1 and/or tnpISEcp1 genes, associated with mobile genetic elements, were identified in all but two isolates. This report describes the first detection of multidrug resistance genes among ESBL-producing E. coli strains retrieved from feces of cattle, pigs, and a wild bird in Greece, underlining their dissemination in diverse ecosystems and emphasizing the need for a One-Health approach when addressing the issue of antimicrobial resistance.

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“…Antimicrobial resistance, and, more specifically, antibiotic resistance in Gram-negative bacteria is a particularly serious public health problem, which poses an urgent need to search for new antibacterial molecules [ 20 ]. Escherichia coli is one of the most studied species worldwide and comprises non-pathogenic bacteria that may act as commensals and belong to the normal intestinal microbiota of humans and many animals; its pathogenic variants are divided into diarrheagenic and extraintestinal pathogens [ 21 ]. In our study, after a preliminary assessment of the antibacterial activity of lavender oil against two strains of B. subtilis and E. coli , chosen as representatives of Gram-positive and Gram-negative bacteria, the attention was focused on the latter species because significant levels of microbial contamination can occur during the use of cosmetic products, and the possible presence of pathogenic organisms pose a potential risk to health.…”

Section: Introductionmentioning

confidence: 99%

Chemical Profile, In Vitro Biological Activity and Comparison of Essential Oils from Fresh and Dried Flowers of Lavandula angustifolia L.

et al. 2021

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The chemical composition of essential oils (EOs) from dried and fresh flowers of Lavandula angustifolia L. (lavender), named LA 2019 and LA 2020, respectively, grown in central Italy was analyzed and compared by GC and GC-MS. For both samples, 61 compounds were identified, corresponding to 97.9% and 98.1% of the total essential oils. Explorative data analysis, performed to compare the statistical composition of the samples, resulted in a high level of global similarity (around 93%). The compositions of both samples were characterized by 10 major compounds, with a predominance of Linalool (35.3–36.0%), Borneol (15.6–19.4%) and 1,8-Cineole (11.0–9.0%). The in vitro antibacterial activity assay by disk diffusion tests against Bacillus subtilis PY79 and Escherichia coli DH5α showed inhibition of growth in both indicator strains. In addition, plate counts revealed a bactericidal effect on E. coli, which was particularly noticeable when using oil from the fresh lavender flowers at the highest concentrations. An in vitro antifungal assay showed that the EOs inhibited the growth of Sclerotium rolfsii, a phytopathogenic fungus that causes post-harvest diseases in many fruits and vegetables. The antioxidant activity was also assessed using the ABTS free radical scavenging assay, which showed a different antioxidant activity in both EOs. In addition, the potential application of EOs as a green method to control biodeterioration phenomena on an artistic wood painting (XIX century) was evaluated.

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Escherichia coli as a Multifaceted Pathogenic and Versatile Bacterium

Cited by 117 publications

References 111 publications

The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37

The Small Metal-Binding Protein SmbP Simplifies the Recombinant Expression and Purification of the Antimicrobial Peptide LL-37

Antimicrobial Resistance Genes in ESBL-Producing Escherichia coli Isolates from Animals in Greece

Chemical Profile, In Vitro Biological Activity and Comparison of Essential Oils from Fresh and Dried Flowers of Lavandula angustifolia L.

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