Esculentoside A specifically binds to ribosomal protein S3a and impairs LPS-induced signaling in macrophages

Li, Yinghua; Wang, Jing; Liu, Ying; Qiu, Lie; Li, Jianzhong; Hu, Honggang; Hu, Zhenlin; Zhang, Wen; Lü, Bin; Zhang, Junping

doi:10.1016/j.intimp.2017.11.018

Cited by 6 publications

(5 citation statements)

References 25 publications

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“…Although RPS3a could enhance the immune system through PAP and proPO, it may not be specifically involved in the host defense mechanism against WSSV infection. Recently, RPS3a has been reported to be required for LPS-triggered signaling during the induction of proinflammatory cytokines, including TNF-α and IL-6 [31]. Therefore, the increased RPS3a expression after WSSV infection in the present study may have occurred as it was required for WSSV to induce the production of proinflammatory cytokines.…”

Section: P Monodonmentioning

confidence: 57%

Effects of ribosomal protein S3a (RPS3a) on the survival of WSSV-infected pacific white shrimp (Litopenaeus vannamei)

Iatsui¹,

Chotigeat²,

Deachamag³

2020

ScienceAsia

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White spot syndrome virus (WSSV) causes substantial economic losses in shrimp farming. It has been reported that shrimp infected with this virus exhibits an overexpression of ribosomal protein S3a (RPS3a). Therefore, a recombinant protein, His-RPS3a, from shrimp was produced and its potential to protect shrimp from WSSV was evaluated. The purified His-RPS3a was injected at concentrations of 1-20 µg/shrimp to determine the expression of the genes prophenoloxidase (proPO) and phagocytosis-activating protein (PAP) by real-time PCR. PAP expression increased 4.1-fold when His-RPS3a was injected at 1 µg/shrimp, and proPO gene expression increased 5.3-fold when His-RPS3a was injected at 20 µg/shrimp. Therefore, shrimp were injected with His-RPS3a at concentrations of 10, 20, or 40 µg/shrimp for 3 days and then challenged with WSSV. The relative percentages of survival (RPS) of the WSSV-challenged shrimp after injection with the His-RPS3a were 23%, 27%, and 35%, respectively. Besides, RPS3a-phMGFP was prepared in the form of chitosan-DNA nanoparticles and fed at 75 µg/shrimp/day for 7 days. PAP expression was found to be higher than that in the control group. Furthermore, shrimp fed with RPS3a-phMGFP DNA at 75 µg/shrimp/day were challenged with WSSV and further cultured for 15 days. The RPS of the WSSV-challenged shrimp after feeding with RPS3a-phMGFP was 35%, whereas the control shrimp exhibited 100% death. These results demonstrated that RPS3a acts as an antigen and could delay the death of WSSV-infected shrimp by activating the general immune system; furthermore, RPS3a may be activated after WSSV infection to induce the production of proinflammatory cytokines.

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Section: P Monodonmentioning

confidence: 57%

Effects of ribosomal protein S3a (RPS3a) on the survival of WSSV-infected pacific white shrimp (Litopenaeus vannamei)

Iatsui¹,

Chotigeat²,

Deachamag³

2020

ScienceAsia

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“…RPS3A belongs to the S3AE family of ribosomal proteins. In addition to its ribosomal function, RPS3A can also perform multiple biological functions unrelated to the ribosomes such as cell proliferation, inflammation, and cellular metabolism (42,43). Several studies have reported that RPS3A is highly expressed in some transformed cells and tumors, and plays a critical role in the regulation of cell proliferation and transformation by exerting extra-ribosomal functions (44)(45)(46).…”

Section: Discussionmentioning

confidence: 99%

Identification of ribosomal protein family as immune-cell-related biomarkers of NAFLD by bioinformatics and experimental analyses

Chen

2023

Front. Endocrinol.

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BackgroundImmune cells play an integral role in the development and progression of non-alcoholic fatty liver disease (NAFLD). This study was to identify immune-cell-related biomarkers for the diagnosis and treatment of NAFLD.Methods and findingsFirst, we introduced human liver transcriptome data from the GEO database (GSE48452 and GSE126848) and performed a weighted gene co-expression network analysis (WGCNA) to screen out the modules related to immune cell infiltration and to identify immune-cell-related differentially expressed genes (ICR-DEGs) associated with NAFLD progression. Further, the protein-protein interaction (PPI) network of ICR-DEGs was established to obtain hub genes and subsequently, the expression trend analysis was conducted to identify immune-cell-related biomarkers of NAFLD. Finally, the mRNA expression of biomarkers was validated in a NAFLD mouse model induced by high-fat diet (HFD) feeding. In total, we identified 66 ICR-DEGs and 13 hub genes associated with NAFLD. Among them, 9 hub genes (CD247, CD74, FCGR2B, IL2RB, INPP5D, MRPL16, RPL35, RPS3A, RPS8) were correlated with the infiltrating immune cells by the Pearson correlation analysis. Subsequently, 4 immune-cell-related biomarkers (RPL35, RPS3A, RPS8, and MRPL16) with the same expression trends in GSE48452 and GSE126848 datasets were identified. These biomarkers were enriched in immune-related pathways and had a good ability to distinguish between NASH and healthy samples. Moreover, we constructed a competing endogenous RNA (ceRNA) network of biomarkers and predicted twenty potential therapeutic drugs targeting RPS3A such as taxifolin and sitagliptin. Finally, experimental validation indicated that the hepatic mRNA expression of Rpl35, Rps3A, and Rps8 was significantly decreased in NAFLD mice.ConclusionsThis study identified four ribosomal protein genes (RPL35, RPS3A, RPS8, and MRPL16) as immune-cell-related biomarkers of NAFLD, which may actively participate in the immune processes during NAFLD progression and could serve as potential targets for the diagnosis and treatment of NAFLD.

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“…18 EsA can inhibit the secretion of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in a dose-dependent manner, which thereby directly reduces the phosphorylation levels of ERK, JNK, and p38 MAPKs. 19,20 Additionally, EsA can also ameliorate the cellular response to different oxidative stresses induced by drugs or chemicals, which prevents cells from inflammation-related apoptosis through the AMPK/Akt/GSK3β pathway. 17,21 Nevertheless, EsA is still limited to a wide range of clinical applications due to its poor stability and toxicity.…”

Section: Introductionmentioning

confidence: 99%

“…EsA prevents acute kidney injury and acute liver injury from the inflammatory response and mitochondrial dysfunction. , Recently, EsA has been proven to play the role of ACE2 inhibitors, thereby acting as a blocker of the spike protein to inhibit SARS-CoV-2 infection and enhance body resistance . EsA can inhibit the secretion of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) in a dose-dependent manner, which thereby directly reduces the phosphorylation levels of ERK, JNK, and p38 MAPKs. , Additionally, EsA can also ameliorate the cellular response to different oxidative stresses induced by drugs or chemicals, which prevents cells from inflammation-related apoptosis through the AMPK/Akt/GSK3β pathway. , Nevertheless, EsA is still limited to a wide range of clinical applications due to its poor stability and toxicity. − …”

Section: Introductionmentioning

confidence: 99%

Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing

Zhu

Shao

et al. 2023

ACS Appl. Nano Mater.

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Burn injury remains one of the most devastating burdens on global public health. The inflammation, caused by burn injury and transplantation of dermal scaffolds, often leads to delayed burn wound healing. Esculentoside A (EsA), with a strong anti-inflammatory capacity, is an available agent that might contribute to the treatment of burn wounds. However, the poor stability and toxicity of EsA limit its clinical application. In the present study, we constructed chitosan/alginate nanoparticles (EsA-CS/ALG-NPs) to improve sustainability and reduce toxicity followed by impregnation of the prepared EsA-CS/ALG-NPs into a collagen/chitosan scaffold (EsA-CS/ALG-NPs@CCS). The particle size, structural morphology, thermal properties, and chemical interaction of repaired nanoparticles were evaluated using the Nanometrics instrument, differential scanning calorimetry, transmission electron microscopy, and Fourier transform infrared spectroscopy, respectively. The hybrid EsA-CS/ALG-NPs@CCS was evaluated for physical characteristics, in vitro drug release, biocompatibility, and anti-inflammation capacity with RAW 264.7 cells and in vivo burn wound healing studies with SD rats. The results showed that we successfully constructed CS/ALG-NPs and optimized the preparation process to achieve the highest encapsulation efficiency. The hybrid EsA-CS/ALG-NPs@CCS, with reduced cytotoxicity and sustained release of EsA, could alleviate inflammation, decrease the ratio of M1 macrophages, and increase the proportion of M2 macrophages in vitro. It was demonstrated that 5 μg EsA CS/ALG-NPs@CCS not only reduces inflammatory cytokines secretion and inhibits M1 macrophages but also promotes the release of anti-inflammatory cytokines and activates M2 macrophages, thereby achieving accelerated and high-quality healing of burn wounds ultimately. In summary, our work suggests that the synergistic combination of EsA, nanoparticles, and scaffolds provided a promising strategy for treating burn injuries.

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Esculentoside A specifically binds to ribosomal protein S3a and impairs LPS-induced signaling in macrophages

Cited by 6 publications

References 25 publications

Effects of ribosomal protein S3a (RPS3a) on the survival of WSSV-infected pacific white shrimp (Litopenaeus vannamei)

Effects of ribosomal protein S3a (RPS3a) on the survival of WSSV-infected pacific white shrimp (Litopenaeus vannamei)

Identification of ribosomal protein family as immune-cell-related biomarkers of NAFLD by bioinformatics and experimental analyses

Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing

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