Esophageal tissue engineering

Luc, G.; Durand, M.; Collet, Denis; Guillemot, Fabien; Bordenave, Laurence

doi:10.1586/17434440.2014.870470

Cited by 15 publications

(13 citation statements)

References 146 publications

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“…At present, even though several methods have been developed to create decellularised scaffolds, no ideal methodology has been described for long-term storage of decellularised oesophagi [18]. Moreover, despite the advances in scaffold development, the feasibility of the process itself in a large animal model has yet to be developed.…”

Section: Introductionmentioning

confidence: 99%

Long-term cryopreservation of decellularised oesophagi for tissue engineering clinical application

et al. 2017

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Oesophageal tissue engineering is a therapeutic alternative when oesophageal replacement is required. Decellularised scaffolds are ideal as they are derived from tissue-specific extracellular matrix and are non-immunogenic. However, appropriate preservation may significantly affect scaffold behaviour. Here we aim to prove that an effective method for short- and long-term preservation can be applied to tissue engineered products allowing their translation to clinical application. Rabbit oesophagi were decellularised using the detergent-enzymatic treatment (DET), a combination of deionised water, sodium deoxycholate and DNase-I. Samples were stored in phosphate-buffered saline solution at 4°C (4°C) or slow cooled in medium with 10% Me2SO at -1°C/min followed by storage in liquid nitrogen (SCM). Structural and functional analyses were performed prior to and after 2 and 4 weeks and 3 and 6 months of storage under each condition. Efficient decellularisation was achieved after 2 cycles of DET as determined with histology and DNA quantification, with preservation of the ECM. Only the SCM method, commonly used for cell storage, maintained the architecture and biomechanical properties of the scaffold up to 6 months. On the contrary, 4°C method was effective for short-term storage but led to a progressive distortion and degradation of the tissue architecture at the following time points. Efficient storage allows a timely use of decellularised oesophagi, essential for clinical translation. Here we describe that slow cooling with cryoprotectant solution in liquid nitrogen vapour leads to reliable long-term storage of decellularised oesophageal scaffolds for tissue engineering purposes.

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Section: Introductionmentioning

confidence: 99%

Long-term cryopreservation of decellularised oesophagi for tissue engineering clinical application

et al. 2017

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“…Des études suggèrent que le GE permet une angiogenèse adaptée à l'organe implanté témoignant d'une grande adaptabilité [130,131]. Dans l'ingénierie tissulaire oesophagienne, l'utilité du GE sur la vascularisation du futur transplant est débattue et peu étudiée [39,132]. L'utilisation du GE dans l'IT oesophagienne permettrait ; la vascularisation du futur transplant limitant ainsi les risques ischémiques, la limitation des réactions inflammatoires, la cellularisation de la matrice avant son implantation.…”

Section: Matrices Résorbables Synthétiquesunclassified

“…L'ajout de cellules quel que soit la matrice utilisée permettrait de réduire l'inflammation, de promouvoir l'angiogénèse et de réduire les sténoses cicatricielles [39]. Les cellules utilisées peuvent être différenciées ou « à potentiel souches ».…”

Section: Cellularisationunclassified

“…préclinique de Tan et al de cellules autologues issues de la moelle osseuse dans un modèle canin. Le groupe expérimental (SIS + cellules) présentait une meilleure cicatrisation et une moindre inflammation que le groupe contrôle (SIS seule) [93].L'utilisation de cellules paraît nécessaire dans les remplacements circulaires oesophagiens sans pour autant clairement identifier le type cellulaire à utiliser[39]. Les Adipose-Derived Stem Cells (ADSCs) apparaissent comme des cellules souches « idéales »[135].…”

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Esophageal Tissue Engineering

Jensen¹,

Sayej²,

Finck³

2017

Frontiers in Stem Cell and Regenerative Medicine Research

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Decellularized matrixes (DM) are commonly used to facilitate a constructive remodeling response in several types of tissue, including the esophagus. Surgical procedure of the esophagus is often complicated by stricture, but preclinical studies have shown that the use of a DM can mitigate stricture and promote a constructive outcome.Recognizing the potential benefits of DM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo remodeling properties of DM from porcine esophagus.The developed protocol for esophageal DM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a good mechanical behavior. Stem cells remained viable when seeded upon the esophageal DM in vitro, and the in-vivo host response showed a pattern of constructive remodeling when implanted in soft tissue.

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“…However, gastrointestinal autografts are limited due to donor site necrosis and various postsurgery complications (Liu, Yang, Zheng, Dong, & Zheng, ; Ludman & Spitz, ). Another strategy involves the use of allografts/xenografts consisting of acellular matrix, but this solution has various limitations, such as immunogenicity and the risk of disease transmission (Luc, Durand, Collet, Guillemot, & Bordenave, ). Therefore, there is increasing interest in oesophageal tissue engineering, which combines biomimetic polymeric scaffolds with autologous cells and is a promising treatment method for oesophageal diseases (Kuppan, Sethuraman, & Krishnan, ; Totonelli et al, ).…”

Section: Introductionmentioning

confidence: 99%

Mechanical stimuli enhance simultaneous differentiation into oesophageal cell lineages in a double‐layered tubular scaffold

Kang

Kim

et al. 2019

J Tissue Eng Regen Med

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The tissue‐engineered oesophagus serves as an alternative and promising therapeutic approach for long‐gap oesophageal replacement. This study proposes an advanced in vitro culture platform focused on construction of the oesophagus by combining an electrospun double‐layered tubular scaffold, stem cells, biochemical reagents, and biomechanical factors. Human mesenchymal stem cells were seeded onto the inner and outer surfaces of the scaffold. Mechanical stimuli were applied with a hollow organ bioreactor along with different biochemical reagents inside and outside of the scaffold. Electrospun fibres in a tubular scaffold were found to be randomly and circumferentially oriented for the inner and outer surfaces, respectively. Amongst the two types of mechanical stimuli, the intermittent shear flow that can simultaneously cause circumferential stretching due to hydrostatic pressure, and shear stress caused by flow on the inner surface, was found to be more effective for simultaneous differentiation into epithelial and muscle lineage than steady shear flow. Under these conditions, the expression of epithelial markers on the inner surface was significantly observed, although it was minimal on the outer surface. Muscle differentiation showed the opposite expression pattern. Meanwhile, the mechanical tests showed that the strength of the scaffold was improved after incubation for 14 days. We have developed a potential platform for tissue‐engineered oesophagus construction. Specifically, simultaneous differentiation into epithelial and muscle lineages can be achieved by utilizing the double‐layered scaffold and appropriate mechanical stimulation.

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Esophageal tissue engineering

Cited by 15 publications

References 146 publications

Long-term cryopreservation of decellularised oesophagi for tissue engineering clinical application

Long-term cryopreservation of decellularised oesophagi for tissue engineering clinical application

Esophageal Tissue Engineering

Mechanical stimuli enhance simultaneous differentiation into oesophageal cell lineages in a double‐layered tubular scaffold

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