Essential oil profiling in callus of some wild and cultivated Daucus genotypes

Jawdat, Dana; Al-Faoury, Hussam; Odeh, Adnan; Al-Rayan, Radwan; Al-Safadi, B.

doi:10.1016/j.indcrop.2016.09.040

Cited by 7 publications

(3 citation statements)

References 46 publications

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“…Almost every culture has documented their use either in the form of root, seeds and leaves to fight against many diseases [1]. Medicinal plants are rich source of phytochemicals, having wide range of anti-microbial, anti-cancerous and anti-tumor applications in medical field [2,3].…”

Section: Introductionmentioning

confidence: 99%

Monochromatic lights-induced trends in antioxidant and antidiabetic polyphenol accumulation in in vitro callus cultures of Lepidium sativum L.

Ullah

Tungmunnithum

Garros

et al. 2019

Journal of Photochemistry and Photobiology B: Biology

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Section: Introductionmentioning

confidence: 99%

Monochromatic lights-induced trends in antioxidant and antidiabetic polyphenol accumulation in in vitro callus cultures of Lepidium sativum L.

Ullah

Tungmunnithum

Garros

et al. 2019

Journal of Photochemistry and Photobiology B: Biology

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“…Plants possess variety of such pharmaceutically important compounds including flavonoids, terpenoids, saponins and phenolics in various plant parts [2]. These phytochemicals possess specific phytochemical properties against cancer, tumor repression, microbes, and viruses [3]. With the advent of modernization, industrialization, and revolution in medicinal field use of pants and their extracts were diseases [1].…”

Section: Introductionmentioning

confidence: 99%

Elicitation, A Mechanistic Approach to Change the Metabolic Pathway of Plants to Produce Pharmacological Important Compounds in In-vitro Cell Cultures

Bajwa¹

2021

GJES

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Plant's secondary metabolites, produced usually under stress, are one of the promising sources for food additives, pharmaceuticals, food flavors and other industrial materials. The comprehensive probing of metabolite's production mechanism, stress signal transduction pathway, would be great push toward in commercial production. Higher plants inevitably encounter stresses and sustain themselves by producing various secondary metabolites which, the secondary metabolites, have various industrial application that's why are promising candidates for commercialization. Due to certain limitations of natural plant extraction, plant cell/tissue culture is considered a best alternative way for in-vitro production of bioactive secondary metabolites. Elicitation can be employed to overcome the constraints of plant cell technology that retard the process of commercialization. A way to enhance the secondary metabolite's production in plants is Elicitation. In which an exogeneous elicitor, biotic or abiotic, is exposed in growth medium to trigger the production of secondary metabolite. During this phenomenon, several defense/ non-defenses related genes, activated/ deactivated. Furthermore, transient phosphorylation or dephosphorylation of proteins, expression of enzymes occurs through which biosynthetic pathways of several secondary metabolites can be ascertained. Additionally, a push toward advancement of metabolic engineering and gene manipulation to increase the productivity of secondary metabolites can be gained through integration of proteomics, transcriptomics, and metabolomics with system biology.

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“…Biosynthesis of medicinally active metabolites in wild grown medicinal plants is limited and affected by many factors, for instance, geographic variations, particular growth and the developmental stage of the plant, specific season, nutrients availability and environmental contamination [17]. However, to combat the environmental and geographic constraints, plant cell culture technology provides promising means for production of healthy plant material with phytochemically sustainable profiles, in short time and limited space [18]. The callus cultures are the preferred type of plant in vitro cultures, used for the production of healthy biomass under controlled growth conditions from which medicinally potent natural products can be extracted [19].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia

Yousaf

Khan

Ullah

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Clinically, available synthetic chemotherapeutics in the treatment for leishmaniasis are associated with serious complications, such as toxicity and emergence of resistance. Natural products from plants can provide better remedies against the Leishmania parasite and can possibly minimize the associated side effects. In this study, various extracts of the callus cultures of Artimisia scoparia established in response to different plant growth regulators (PGRs) were evaluated for their anti-leishmanial effects against Leishmania tropica promastigotes, followed by an investigation of the possible mechanism of action through reactive apoptosis assay using fluorescent microscopy. Amongst the different callus extracts, higher anti-leishmanial activity (IC 50 :19.13 mg/mL) was observed in the callus raised in-vitro in the presence of 6-Benzylaminopurine (BA) plus 2,4-Dichlorophenoxyacetic Acid (2,4-D) at the concentration of 1.5 mg/L, each. Further, the results of apoptosis assay showed a large number of early-stage apoptotic (EA) and late-stage apoptotic (LA) cells in the Leishmania under the effect of callus extract grown in-vitro at BA plus 2,4-D. For the determination of the potent natural products in the callus extracts responsible for the anti-leishmanial activity, extracts were subjected to Gas chromatography-mass spectrometry (GC-MS) for the metabolite analysis. Nonetheless, higher levels of the metabolites, such as nerolidol (22%), pelletierine (18%), aspidin (15%) and ascaridole (11%) were detected in the callus grown in vitro at BA plus 2,4-D (1.5 mg/L, each). This protocol determines a novel method of production of anti-leishmanial natural products through callus cultures of A. scoparia, a medicinal plant.

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Essential oil profiling in callus of some wild and cultivated Daucus genotypes

Cited by 7 publications

References 46 publications

Monochromatic lights-induced trends in antioxidant and antidiabetic polyphenol accumulation in in vitro callus cultures of Lepidium sativum L.

Monochromatic lights-induced trends in antioxidant and antidiabetic polyphenol accumulation in in vitro callus cultures of Lepidium sativum L.

Elicitation, A Mechanistic Approach to Change the Metabolic Pathway of Plants to Produce Pharmacological Important Compounds in In-vitro Cell Cultures

Biosynthesis of anti-leishmanial natural products in callus cultures of Artemisia scoparia

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