Essential oil yield and quality in rose-scented geranium: Variation among clones and plant parts

Ravindra, N. S.; Kulkarni, R. N.

doi:10.1016/j.scienta.2014.12.023

Cited by 20 publications

(10 citation statements)

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“…Terpenes are the largest and the most diverse class of natural products, and constitute a major component of essential oil in rose-scented geranium. They are produced as a homologous series of molecules as polymers of isoprene, the C 5 precursor molecules being IPP and/or DMAPP that are generated via the process of isoprenogenesis [11, 26]. In plants, isoprenogenesis occurs through two discrete biosynthetic pathways: the mevalonic acid (MVA) pathway in cytosol and the 2-C-methyl-D-erythritol 4-phosphate/1-deoxy-D-xylulose 5-phosphate (MEP/DOXP) pathway in plastids.…”

Section: Resultsmentioning

confidence: 99%

“…The main components which determine its aroma are citronellol, geraniol, linalool and their esters [34]. In addition, significant quantities of isomenthone, menthone, nerol, cis -and trans -rose oxides, α-terpineol, α -pinene, myrcene, and β-phyllandrene contributes to its aroma [26]. In agreement with the aroma profile of this plant, significant level of expression was observed for the putative genes encoding geraniol synthase, linalool synthase, myrcene synthase, β-ocimene synthase, limonene synthase, germacrene synthase, nerolidol synthase, cadinene synthase, copalyl diphosphate synthase, kaurene synthase, and BAHD acyltransferase.…”

Section: Resultsmentioning

confidence: 99%

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De novo transcriptome analysis of rose-scented geranium provides insights into the metabolic specificity of terpene and tartaric acid biosynthesis

et al. 2017

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BackgroundRose-scented geranium (Pelargonium sp.) is a perennial herb that produces a high value essential oil of fragrant significance due to the characteristic compositional blend of rose-oxide and acyclic monoterpenoids in foliage. Recently, the plant has also been shown to produce tartaric acid in leaf tissues. Rose-scented geranium represents top-tier cash crop in terms of economic returns and significance of the plant and plant products. However, there has hardly been any study on its metabolism and functional genomics, nor any genomic expression dataset resource is available in public domain. Therefore, to begin the gains in molecular understanding of specialized metabolic pathways of the plant, de novo sequencing of rose-scented geranium leaf transcriptome, transcript assembly, annotation, expression profiling as well as their validation were carried out.Results De novo transcriptome analysis resulted a total of 78,943 unique contigs (average length: 623 bp, and N50 length: 752 bp) from 15.44 million high quality raw reads. In silico functional annotation led to the identification of several putative genes representing terpene, ascorbic acid and tartaric acid biosynthetic pathways, hormone metabolism, and transcription factors. Additionally, a total of 6,040 simple sequence repeat (SSR) motifs were identified in 6.8% of the expressed transcripts. The highest frequency of SSR was of tri-nucleotides (50%). Further, transcriptome assembly was validated for randomly selected putative genes by standard PCR-based approach. In silico expression profile of assembled contigs were validated by real-time PCR analysis of selected transcripts.ConclusionBeing the first report on transcriptome analysis of rose-scented geranium the data sets and the leads and directions reflected in this investigation will serve as a foundation for pursuing and understanding molecular aspects of its biology, and specialized metabolic pathways, metabolic engineering, genetic diversity as well as molecular breeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-3437-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

De novo transcriptome analysis of rose-scented geranium provides insights into the metabolic specificity of terpene and tartaric acid biosynthesis

et al. 2017

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“…Furthermore, investigating essential oils yield could prove important in terms of its economic relevance. However, some authors argue that the content of essential oils is more important than the yield, as content determines the market price for farmers and the marketability of fresh plants for different enduses (Ravindra and Kulkarni, 2015). Other authors argue that it is desirable to change the harvest season of certain aromatic plants to the phase after blooming in order to obtain better-quality oil, even at the cost of decreasing yield (Salgueiro et al, 2010).…”

Section: Nd Harvestmentioning

confidence: 99%

Composition variation of the essential oil from Ocimum basilicum L. cv. Genovese Gigante in response to Glomus intraradices and mild water stress at different stages of growth

Mota

Sánchez-Sánchez

Pedro

et al. 2020

Biochemical Systematics and Ecology

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In this study, a factorial experiment was carried out to investigate the effect of AMF (arbuscular mycorrhizal fungi) and water stress on the quality of fresh plants (through the analysis of essential oil composition) and also on the morphological characteristics (in terms of biomass) of basil (Ocimum basilicum L. cv. Genovese Gigante). The factors included an arbuscular mycorrhizal fungus inoculation (Glomus intraradices), irrigation level (mild water stress, 60% Field capacity) and the interaction of both AMF and water stress. In addition, the effects of harvesting at two different developmental stages were assessed with respect to the plants tolerance to abiotic stress and the quality of the fresh plant. The main essential oil (EO) constituents for this cultivar were eugenol, which varied between 7.1 and 50.8%, and linalool, 17.0-54.7%, in all the samples. The highest relative amount of eugenol (50.8%) was obtained during the vegetative stage in plants under water stress whereas samples from the control presented 16.2% for this compound. The samples which were significantly different from the control samples were the WS (water stress) samples from the vegetative stage. Additionally, this study suggests that AMF plants offset the adverse effect of water stress. In this context, and since the interaction of AMF and water stress did not significantly change the fresh plant quality, i.e. the essential oil profile in both stages of development, it is worthwhile considering this method of production. Finally, this experiment permitted an understanding of the stressor thresholds of the species used and also that, compensatory responses occur at different time scales, including between generations and also during development (developmental plasticity).

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“…In India, geranium was introduced in the L. K. Narnoliya • J. S. Jadaun • S. P. Singh (*) Center of Innovative and Applied Bioprocessing (CIAB), Mohali, India e-mail: sudhirsingh@ciab.res.in nineteenth century in the southern climate, and now it grows in different parts of India. They are also used as ornamental plants, for example, Pelargonium x hortorum and Pelargonium graveolens, and grown in gardens and parks to provide a pleasant fragrance (Ravindra and Kulkarni 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Generally, they play a crucial role in ecological adjustment of the plant and protect it from pathogen and herbivore attacks. Thus, essential oil components are the key substances of rose-scented geranium for its defence system (Babu and Kaul 2005;Jadaun et al 2017;Ravindra and Kulkarni 2015).…”

Section: Introductionmentioning

confidence: 99%

The Phytochemical Composition, Biological Effects and Biotechnological Approaches to the Production of High-Value Essential Oil from Geranium

Narnoliya

Jadaun

Singh

2019

Essential Oil Research

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Essential oil yield and quality in rose-scented geranium: Variation among clones and plant parts

Cited by 20 publications

References 13 publications

De novo transcriptome analysis of rose-scented geranium provides insights into the metabolic specificity of terpene and tartaric acid biosynthesis

De novo transcriptome analysis of rose-scented geranium provides insights into the metabolic specificity of terpene and tartaric acid biosynthesis

Composition variation of the essential oil from Ocimum basilicum L. cv. Genovese Gigante in response to Glomus intraradices and mild water stress at different stages of growth

The Phytochemical Composition, Biological Effects and Biotechnological Approaches to the Production of High-Value Essential Oil from Geranium

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