Essential oils of Origanum compactum increase membrane permeability, disturb cell membrane integrity, and suppress quorum-sensing phenotype in bacteria

Bouyahya, Abdelhakim; Abrini, Jamal; Dakka, Nadia; Bakri, Youssef

doi:10.1016/j.jpha.2019.03.001

Cited by 144 publications

(98 citation statements)

References 47 publications

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“…These results support the assumption that both strains grow more slowly in the presence of the OEO, and that they need a longer time to adapt to the environment as the OEO concentration increases. It is likely that EO is disturbing the cell membrane integrity and increasing the membrane permeability of both strains, thereby leading to a prolonged adaptation and lag phase time [ 35 ]. Comparing SaROEO to SaWT, no significant differences ( p < 0.05) were observed when strains were grown in absence of OEO.…”

Section: Discussionmentioning

confidence: 99%

“…Although differences were observed in both parameters, SaROEO results revealed a greater relevance of lag phase in the bacterial adaptation to OEO than in the adaptation of S. enterica to carvacrol. One of the main bacterial targets of EO is the cell membrane [ 35 ]; it is likely that mutations in SaROEO are related to the cell envelopes, which allow for a faster adaptation to OEO and consequently a decrease in lag phase time. These results would thus explain why mutations of SaROEO were fixed in the bacterial population after evolution assay (750 µL/L OEO).…”

Section: Discussionmentioning

confidence: 99%

“…According to Meades, et al [ 52 ], cinnamon EO inhibits the carboxyltransferase subunit of E. coli , which partially explains that EO’s antibacterial activity. Moreover, as is well known, cell membranes are the first barrier and one of the main structures affected by natural antimicrobials, which alter their permeability and disrupt their integrity [ 35 , 47 ]. It is likely that the mutation of accA could result in improved cell membrane formation and/or repair in presence of OEO.…”

Section: Discussionmentioning

confidence: 99%

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Incubation with a Complex Orange Essential Oil Leads to Evolved Mutants with Increased Resistance and Tolerance

et al. 2020

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Emergence of strains with increased resistance/tolerance to natural antimicrobials was evidenced after cyclic exposure to carvacrol, citral, and (+)-limonene oxide. However, no previous studies have reported the development of resistance and tolerance to complex essential oils (EOs). This study seeks to evaluate the occurrence of Staphylococcus aureus strains resistant and tolerant to a complex orange essential oil (OEO) after prolonged cyclic treatments at low concentrations. Phenotypic characterization of evolved strains revealed an increase of minimum inhibitory and bactericidal concentration for OEO, a better growth fitness in presence of OEO, and an enhanced survival to lethal treatments, compared to wild-type strain. However, no significant differences (p > 0.05) in cross-resistance to antibiotics were observed. Mutations in hepT and accA in evolved strains highlight the important role of oxidative stress in the cell response to OEO, as well as the relevance of the cell membrane in the cell response to these natural antimicrobials. This study demonstrates the emergence of S. aureus strains that are resistant and tolerant to EO (Citrus sinensis). This phenomenon should be taken into account to assure the efficacy of natural antimicrobials in the design of food preservation strategies, in cleaning and disinfection protocols, and in clinical applications against resistant bacteria.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Incubation with a Complex Orange Essential Oil Leads to Evolved Mutants with Increased Resistance and Tolerance

et al. 2020

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“…The antiviral mechanisms of different extracts preparations of many vegetal products have been by the disturbance of cell membrane integrity thereby increasing the membrane permeability, and invariably causing the leakage of the RNA or DNA of the virus ( Bouyahya et al., 2019 ), whereas fortifying or strengthening the cell membrane with nutraceuticals that offer protection to the integrity promises to be a functional approach to preventing invasion by known viruses and by extension the novel SARS-COV-2. Among the protective vegetal natural products reported to preserve or enhance cell membrane integrity are the following, some of which also have antiviral activities against respiratory viruses causing flu (gripe), while a few others have been studied against the earlier members of the coronavirus family such as the MERS and SARS.…”

Section: Medicinal Plants With Pharmacological and Biological Action mentioning

confidence: 99%

A systematic review on COVID-19 pandemic with special emphasis on curative potentials of Nigeria based medicinal plants

Oladele¹,

Ajayi

Oyeleke³

et al. 2020

Heliyon

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Despite the frightening mortality rate associated with COVID-19, there is no known approved drug to effectively combat the pandemic. COVID-19 clinical manifestations include fever, fatigue, cough, shortness of breath, and other complications. At present, there is no known effective treatment or vaccine that can mitigate/inhibit SARS-CoV-2. Available clinical intervention for COVID-19 is only palliative and limited to support. Thus, there is an exigent need for effective and non-invasive treatment. This article evaluates the possible mechanism of actions of SARS-CoV-2 and present Nigeria based medicinal plants which have pharmacological and biological activities that can mitigate the hallmarks of the pathogenesis of COVID-19. SARS-CoV-2 mode of actions includes hyper-inflammation characterized by a severe and fatal hyper-cytokinaemia with multi-organ failure; immunosuppression; reduction of angiotensin-converting enzyme 2 (ACE2) to enhance pulmonary vascular permeability causing damage to the alveoli; and further activated by open reading frame (ORF)3a, ORF3b, and ORF7a via c-Jun N- terminal kinase (JNK) pathway which induces lung damage. These mechanisms of action of SARS-CoV-2 can be mitigated by a combination therapy of medicinal herbs based on their pharmacological activities. Since the clinical manifestations of COVID-19 are multifactorial with co-morbidities, we strongly recommend the use of combined therapy such that two or more herbs with specific therapeutic actions are administered to combat the mediators of the disease.

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“…Origanum compactum (O. compactum) is an endemic Moroccan medicinal herb. Numerous studies have shown that the O. compactum organic extracts, essential oil and its main components possess a broader spectrum of pharmacological and therapeutic activities such as antibacterial, antifungal, antioxidant and anticancer activity (5,6). O. compactum, locally known as za'tar in Morocco, is a culinary spice but also used in the treatment of many diseases such as colitis, bronchopulmonary, gastric acidity and gastrointestinal diseases (7,8).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial activity of Origanum compactum essential oil tested on vaginal and cervical clinical bacterial strains

Ibišević¹,

Husejnagić²,

Kazanović³

et al. 2019

Acta fac medic Naissensis

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Origanum compactum (O. compactum) is an endemic Moroccan medicinal herb. Numerous studies have shown that O. compactum organic extracts, essential oil and its main components possess a broader spectrum of pharmacological and therapeutic activities such as antibacterial, antifungal, antioxidant and antitumour activity. This research was designed to examine the antibacterial activity of O. compactum essential oil tested on clinical bacterial strains isolated from vaginal and cervical swabs. First, antibacterial activity was tested against standard bacterial cultures: Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 51299, Escherichia coli ATCC 25922, and after that on clinical strains. For testing the antibacterial activity, agar diffusion and microdilution methods were used. The inhibition zones (IZ) for standard bacterial cultures were from 31.0 ± 0.57 mm to 35.0 ± 1.15 mm. The minimum inhibitory concentracion (MIC) for essential oil was tested using the broth dilution method. The values were in the range of 0.098 mg/ml-1.562 mg/ml. O. compactum essential oil provided strong antibacterial activity for all tested microorganisms. The antibacterial activity of essential oil depends largely on the main components: carvacrol and thymol. Clinical isolates, which are more resistant in comparison with laboratory strains, are almost equally sensible to O. compactum essential oil. This essential oil could be an ideal replacement for conventional antimicrobial products, especially if we consider the increasing resistance to implemented antibiotics. In the future, O. compactum essential oil could be an option in the treatment of gynecological infections.

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Essential oils of Origanum compactum increase membrane permeability, disturb cell membrane integrity, and suppress quorum-sensing phenotype in bacteria

Cited by 144 publications

References 47 publications

Incubation with a Complex Orange Essential Oil Leads to Evolved Mutants with Increased Resistance and Tolerance

Incubation with a Complex Orange Essential Oil Leads to Evolved Mutants with Increased Resistance and Tolerance

A systematic review on COVID-19 pandemic with special emphasis on curative potentials of Nigeria based medicinal plants

Antibacterial activity of Origanum compactum essential oil tested on vaginal and cervical clinical bacterial strains

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