Essential role for SNMP1 in detection of sex pheromones in Helicoverpa armigera

Liu, Shuai; Chang, Hetan; Liu, Wei; Cui, Weichan; Liu, Yang; Wang, Yinliang; Ren, Bingzhong; Wang, Guirong

doi:10.1016/j.ibmb.2020.103485

Cited by 20 publications

(24 citation statements)

References 54 publications

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“…RT-PCR results further validated RNA-Seq data of 3 DabiSNMPs. Moreover, DabiSNMP1 appeared to have a higher transcription in male antennae compared to females (Figure 8B), suggesting its putative olfactory roles in the sensing of female sex pheromones in D. abietella, coupled with SNMP1 functions in previous studies (Pregitzer et al, 2014;Liu S. et al, 2020). For the expression of DabiSNMP3 in female and male abdomens (Figure 8B), we suggested that its transcripts may be restricted to midguts of both sexes, as its orthologous genes have been demonstrated to be midgut-specific relatives in B. mori and H. armigera (Zhang et al, 2020;Xu et al, 2021).…”

Section: Phylogenetic and Expression Profiling Analysis Of Dioryctria Abietella Sensory Neuron Membrane Proteinsmentioning

confidence: 55%

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Wang

Chun

et al. 2021

Front. Ecol. Evol.

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In Lepidoptera, RNA sequencing has become a useful tool in identifying chemosensory genes from antennal transcriptomes, but little attention is paid to non-antennal tissues. Though the antennae are primarily responsible for olfaction, studies have found that a certain number of chemosensory genes are exclusively or highly expressed in the non-antennal tissues, such as proboscises, legs and abdomens. In this study, we report a global transcriptome of 16 tissues from Dioryctria abietella, including chemosensory and non-chemosensory tissues. Through Illumina sequencing, totally 952,658,466 clean reads were generated, summing to 142.90 gigabases of data. Based on the transcriptome, 235 chemosensory-related genes were identified, comprising 42 odorant binding proteins (OBPs), 23 chemosensory proteins (CSPs), 75 odorant receptors (ORs), 62 gustatory receptors (GRs), 30 ionotropic receptors (IRs), and 3 sensory neuron membrane proteins (SNMPs). Compared to a previous study in this species, 140 novel genes were found. A transcriptome-wide analysis combined with PCR results revealed that except for GRs, the majority of other five chemosensory gene families in Lepidoptera were expressed in the antennae, including 160 chemosensory genes in D. abietella. Using phylogenetic and expression profiling analyses, members of the six chemosensory gene repertoires were characterized, in which 11 DabiORs were candidates for detecting female sex pheromones in D. abietella, and DabiOR23 may be involved in the sensing of plant-derived phenylacetaldehyde. Intriguingly, more than half of the genes were detected in the proboscises, and one fourth of the genes were found to have the expression in the legs. Our study not only greatly extends and improves the description of chemosensory genes in D. abietella, but also identifies potential molecular targets involved in olfaction, gustation and non-chemosensory functions for control of this pest.

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Section: Phylogenetic and Expression Profiling Analysis Of Dioryctria Abietella Sensory Neuron Membrane Proteinsmentioning

confidence: 55%

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Wang

Chun

et al. 2021

Front. Ecol. Evol.

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“…After starvation for 6 h, 2‐d‐old adults were used in the wind tunnel assay following the methods from previous reports (Liu et al ., 2020; Tang et al ., 2020). The size of the wind tunnel device is 150 cm × 60 cm × 60 cm (Noldus, Netherlands).…”

Section: Methodsmentioning

confidence: 99%

“…The cotton bollworm Helicoverpa armigera is an important agricultural pest, which damages a wide range of host plants. ORs for pheromones and plant VOCs have been well‐studied in this specie, but acid sensing by means of IRs is unknown (Cao et al ., 2016; Chang et al ., 2017; Guo et al ., 2018; Cao et al ., 2020; Guo et al ., 2020; Liu et al ., 2020). In this study, clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR‐associated protein 9 (Cas9)‐mediated genome editing was employed to knockout HarmIR8a for in vivo functional analysis.…”

Section: Introductionmentioning

confidence: 99%

Ionotropic receptor 8a is involved in the attraction of Helicoverpa armigera to acetic acid

et al. 2021

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Ionotropic receptors (IRs) were first found in Drosophila melanogaster, and derive from ionotropic glutamate receptors (iGluRs), which are implicated in detecting acids, ammonia, amine, temperature and humidity. Although IRs are involved in sensing acid odors in a few insects, such as D. melanogaster, Aedes aegypti, and Manduca sexta, the function of IRs in Helicoverpa armigera is still unknown. IR8a was confirmed to be a co‐receptor associated with acid detection. From the results of phylogenetic analysis, HarmIR8a displayed high similarity compared to homologs in D. melanogaster, M. sexta, and A. aegypti, suggesting that HarmIR8a might have a consistent function as a co‐receptor for acid detection. In this study, clustered regularly interspaced palindromic repeats (CRISPR) / CRISPR‐associated protein 9 (Cas9)‐mediated genome editing was implemented to knockout HarmIR8a for in vivo functional analysis. Electrophysiological and behavioral assays were performed to compare the differences between HarmIR8a knockout mutants and wild type individuals. From electroantennogram (EAG) analysis, we found that wild type H. armigera adults could detect short‐chain carboxylic acids. In addition, wind tunnel experiments showed that 1% acetic acid attracted wild type H. armigera adults. However, acid sensing and attraction were reduced or abolished in the HarmIR8a knockout mutants. Our data suggest that HarmIR8a is important for H. armigera to detect short‐chain carboxylic acids and mediate attraction behavior to acetic acid.

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“…the excellent review by Cassau and Krieger [ 94 ]), and their role in pheromone signaling has been noticed [ 95 ]. Very recently, it has been disclosed that a new sensory membrane protein, HarmSNMP1, plays an essential role in the detection of long-chain sex pheromones in Helicoverpa armigera, but this protein was not required for detecting shorter chain sex pheromones of the same species [ 96 ]. Zhao et al [ 97 ] reported the identification of 49 OBPs and 5 chemosensory proteins (CSPs) in the chive gnat Bradysia odoriphaga , and Zhang et al [ 98 ] identified 64 ORs, 24 OBPs and 19 CSPs after analyzing transcripts expressed in chemosensory organs of the beet armyworm S. exigua.…”

Section: Mechanisms Of Insect Sex Pheromone Perceptionmentioning

confidence: 99%

Latest Developments in Insect Sex Pheromone Research and Its Application in Agricultural Pest Management

Rizvi

George

Reddy

et al. 2021

Insects

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Since the first identification of the silkworm moth sex pheromone in 1959, significant research has been reported on identifying and unravelling the sex pheromone mechanisms of hundreds of insect species. In the past two decades, the number of research studies on new insect pheromones, pheromone biosynthesis, mode of action, peripheral olfactory and neural mechanisms, and their practical applications in Integrated Pest Management has increased dramatically. An interdisciplinary approach that uses the advances and new techniques in analytical chemistry, chemical ecology, neurophysiology, genetics, and evolutionary and molecular biology has helped us to better understand the pheromone perception mechanisms and its practical application in agricultural pest management. In this review, we present the most recent developments in pheromone research and its application in the past two decades.

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Essential role for SNMP1 in detection of sex pheromones in Helicoverpa armigera

Cited by 20 publications

References 54 publications

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Transcriptome Analysis and Characterization of Chemosensory Genes in the Forest Pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Ionotropic receptor 8a is involved in the attraction of Helicoverpa armigera to acetic acid

Latest Developments in Insect Sex Pheromone Research and Its Application in Agricultural Pest Management

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