Establishing a highly efficient Agrobacterium-mediated transformation system in sweet buckwheat

Zhou, Baoyuan; Ding, Fan; Zhu, Youyong; Song, Zhenzhen; Ji, Shunrong; Wang, Shuping; Xue, Wei; Liu, Zhixiong; Fang, Zhengwu; Zhang, Yingxin

doi:10.1007/s00003-023-01444-x

Cited by 3 publications

(1 citation statement)

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“…For F. esculentum, an alternative approach of transformation is also available. A recently published Agrobacterium-mediated floral dip method, utilising a GFP-expressing vector, achieved a transformation efficiency of up to 12% (Zhou et al, 2023). While this work represents significant progress in the genetic manipulation of F. tataricum, the presented protocol has limitations due to intrinsic differences among cultivars and their ability to form callus.…”

Section: Discussionmentioning

confidence: 99%

Efficient Agrobacterium-mediated transformation and genome editing of Fagopyrum tataricum

Pinski,

Betekhtin

2023

Front. Plant Sci.

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Fagopyrum tataricum (L.) Gaertn. is an exceptional crop known for its remarkable health benefits, high levels of beneficial polyphenols and gluten-free properties, making it highly sought-after as a functional food. Its self-fertilisation capability and adaptability to challenging environments further contribute to its potential as a sustainable agricultural option. To harness its unique traits, genetic transformation in F. tataricum is crucial. In this study, we optimised the Agrobacterium-mediated transformation protocol for F. tataricum callus, resulting in a transformation rate of regenerated plants of approximately 20%. The protocol’s effectiveness was confirmed through successful GUS staining, GFP expression, and the generation of albino plants via FtPDS gene inactivation. These results validate the feasibility of genetic manipulation and highlight the potential for trait enhancement in F. tataricum.

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Section: Discussionmentioning

confidence: 99%