Establishing a UHPLC-MS/MS method for evaluation of the influence of stir-frying on the pharmacokinetics of seven compounds in Arctii Fructus

Shi, Yun; Jing, Hu; Wang, Hongsen; Yan, Zhankuan; Zhao, Guangrui; Gao, Xun; Li, Weidong; Qin, Kunming

doi:10.1039/d2ra03637a

Cited by 4 publications

(1 citation statement)

References 15 publications

(12 reference statements)

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“…Due to its high-resolution ratio, selectivity, and reproducibility, RP-HPLC is the most extensive and central technique in the analysis and separation of a wide range of compounds and the study of the pKa values of drug molecules ( D'Archivio, 2019 ; Yılmaz Ortak and Cubuk Demiralay, 2019 ). Apart from molecular structure, numerous factors in chromatographic analysis programs have an important influence on retention time, such as the pH of the mobile phase, column temperature, mobile phase composition, and type of chromatographic column ( Huang et al, 2019 ; Tsui et al, 2019 ; Annadi et al, 2022 ; Shi et al, 2022 ). The chromatographic conditions can be adjusted and optimized to achieve satisfactory separation of mixtures and symmetric peak shapes.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous effect of different chromatographic conditions on the chromatographic retention of pentapeptide derivatives (HGRFG and NPNPT)

et al. 2023

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Introduction: Oligopeptides exhibit great prospects for clinical application and its separation is of great importance in new drug development.Methods: To accurately predict the retention of pentapeptides with analogous structures in chromatography, the retention times of 57 pentapeptide derivatives in seven buffers at three temperatures and four mobile phase compositions were measured via reversed-phase high-performance liquid chromatography. The parameters (kHA, kA, and pKa) of the acid–base equilibrium were obtained by fitting the data corresponding to a sigmoidal function. We then studied the dependence of these parameters on the temperature (T), organic modifier composition (φ, methanol volume fraction), and polarity (PmN parameter). Finally, we proposed two six-parameter models with (1) pH and T and (2) pH and φ or PmN as the independent variables. These models were validated for their prediction capacities by linearly fitting the predicted retention factor k-value and the experimental k-value.Results: The results showed that logkHA and logkA exhibited linear relationships with 1/T, φ or PmN for all pentapeptides, especially for the acid pentapeptides. In the model of pH and T, the correlation coefficient (R2) of the acid pentapeptides was 0.8603, suggesting a certain prediction capability of chromatographic retention. Moreover, in the model of pH and φ or PmN, the R2 values of the acid and neutral pentapeptides were greater than 0.93, and the average root mean squared error was approximately 0.3, indicating that the k-values could be effectively predicted.Discussion: In summary, the two six-parameter models were appropriate to characterize the chromatographic retention of amphoteric compounds, especially the acid or neutral pentapeptides, and could predict the chromatographic retention of pentapeptide compounds.

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Section: Introductionmentioning

confidence: 99%

Simultaneous effect of different chromatographic conditions on the chromatographic retention of pentapeptide derivatives (HGRFG and NPNPT)

et al. 2023

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Dominant lignan profiles and antidiabetic activity of thermally treated and non-treated burdock seeds

Pljevljakušić,

Živković,

Petričević

et al. 2024

Industrial Crops and Products

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A systematic review on botany, ethnopharmacology, quality control, phytochemistry, pharmacology and toxicity of Arctium lappa L. fruit

Jin

Liu

Chen

et al. 2023

Journal of Ethnopharmacology

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Establishing a UHPLC-MS/MS method for evaluation of the influence of stir-frying on the pharmacokinetics of seven compounds in Arctii Fructus

Abstract: The pharmacokinetics of multiple, orally administrated compounds of raw and stir-fried Arctii Fructus extracts using a UHPLC-MSMS method.

Cited by 4 publications

References 15 publications

Simultaneous effect of different chromatographic conditions on the chromatographic retention of pentapeptide derivatives (HGRFG and NPNPT)

Simultaneous effect of different chromatographic conditions on the chromatographic retention of pentapeptide derivatives (HGRFG and NPNPT)

Dominant lignan profiles and antidiabetic activity of thermally treated and non-treated burdock seeds

A systematic review on botany, ethnopharmacology, quality control, phytochemistry, pharmacology and toxicity of Arctium lappa L. fruit

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