Establishing an inexpensive, space efficient colony of <i>Bemisia tabaci</i> MEAM1 utilizing modelling and feedback control principles

Thompson, Natalie M.; Waterton, Nadia; Armaou, Antonios; Polston, Jane E.; Curtis, Wayne R.

doi:10.1111/jen.12995

Cited by 6 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Viability Assay: For further assessment of MHPs, the health of the whiteflies at the end of the accumulation time course was assessed using a quantitative fecundity bioassay on cabbage as described elsewhere 11 . In brief, the GA7 tissue culture vessel was introduced into a screen-cage to release the whiteflies onto cabbage to allow proliferation on this favorable host plant.…”

Section: Resultsmentioning

confidence: 99%

“…Viability derived from kinetic model: Since the total fly harvest method does not distinguish the healthy whitefly population from the dead/dying whiteflies at the harvested endpoint, we used a kinetic bioassay model to back-calculate the viable whiteflies 11 . This deterministic kinetic model was based on non-axenic whitefly colony proliferation characterized by image analysis (ImageJ, 64-bit Java 1.8.0_172) of the cabbage plant surface area as well as automated counting of whiteflies after 6 weeks of proliferation.…”

Section: Methodsmentioning

confidence: 99%

“…Notably, maintaining insects on plants for entomological study in growth chambers or greenhouses is expensive and prone to contamination with other arthropods and plant diseases. Previous efforts resulted in reductions in effort and cost of whitefly colony maintenance by an order of magnitude 11 . Beyond the expense of multiorganism maintenance, the mobility of insects presents a significant consideration for biocontainment.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Enabling biocontained plant virus transmission studies through establishment of an axenic whitefly (Bemisia tabaci) colony on plant tissue culture

Curtis,

Thompson,

Krum

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Whiteflies (Bemisia tabaci) and the diseases they transmit are active enemies of global crop yield and therefore multidisciplinary studies involving plant-insect-virus interactions are needed. Presented here is the establishment of a colony of axenic (culturable microorganism free) whiteflies on tissue-cultured plants. Colony establishment was achieved with a surface sterilization of whitefly eggs laid on axenic phototrophically tissue-cultured plants. The recovery and transfer of emerging whiteflies through coupled tissue culture vessels to new axenic plants allows robust subculturing and produces hundreds of whitefly adults per month in a 1.1 L tissue culture vessel. Whitefly proliferation on more than a dozen plant species is shown as well as in vitro testing of whitefly preference for different plants. This novel axenic system provides the high-level of biocontainment needed to conduct virus transmission experiments. Axenic whitefly adults were able to acquire and transmit a begomovirus from tissue-cultured plants, indicating the irrelevance of culturable gut microorganisms in virus transmission. The methodology described here could be used to address a wide range of questions regarding whiteflies and plant virus transmission without the expense, facilities, and contamination risks associated with standard approaches.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Enabling biocontained plant virus transmission studies through establishment of an axenic whitefly (Bemisia tabaci) colony on plant tissue culture

Curtis,

Thompson,

Krum

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Viability derived from kinetic model: Since the total ies harvested did not distinguish the healthy white y population from the dead/dying white ies at the harvested endpoint, we used a kinetic bioassay model to back-calculate the viable white ies (Thompson et al 2022). This deterministic kinetic model was based on non-axenic white y colony proliferation characterized by image analysis (ImageJ, 64-bit Java 1.8.0_172) of the cabbage plant surface area as well as automated counting of white ies after 6 weeks of proliferation.…”

Section: Methodsmentioning

confidence: 99%

Enabling biocontained plant virus transmission studies through establishment of axenic whitefly (Bemisia tabaci) colony on plant tissue culture

Thompson,

Krum,

Chen

et al. 2023

Preprint

View full text Add to dashboard Cite

A colony of axenic ‘sweet potato’ whiteflies (i.e., free from culturable microorganisms) was established on tissue-cultured plants and was used to demonstrate in vitro begomovirus transmission. A co-culture system based on non-viral host cabbage (Brassica oleracea) provides for efficient maintenance of a whitefly (Bemisia tabaci) colony. The recovery of emerging whiteflies through coupled tissue culture vessels to new axenic plants allows robust subculturing and facilitates the production of hundreds of whitefly adults per month in a 1.1 L tissue culture vessel. This novel ‘communal axenic’ plant-insect-virus (aP-I-V) protocol provides high-level biocontainment to allow for work with genetically modified viruses. Versatility is illustrated by achieving whitefly proliferation on more than a dozen plant species as well as methods for in vitro testing of whitefly preference for different plants – although there were large differences in whitefly accumulation and ease of host use. Although initial colony establishment was achieved with surface sterilization of detached whitefly eggs on vegetatively propagated sweet potato (Ipomoea batatas), a much more reliable approach is described based on non-axenic whitefly egg-lay onto axenic phototrophically tissue-cultured plants, followed by surface sterilization of the pedicel-attached eggs. Axenic whitefly adults were able to acquire and transmit tomato mottle virus (ToMoV) (Genus: begomovirus) from Nicotiana benthamiana tissue cultured material. This indicated the absence of a role for culturable gut microorganisms in the transmission of this begomovirus. Acquisition occurred from tissue-cultured plants initially inoculated with ToMoV via Agrobacterium infective clones as well as from a gamma irradiated sachet of parafilm. An autoclavable membrane device was developed to facilitate a simpler approach to undertake virus transmission in biocontained, axenic plant tissue culture. The methodology described here could be used to address a wide range of questions regarding whitefly feeding on plants as well as their transmission of viruses without the expense, facilities, and contamination risks associated with standard approaches.

show abstract

“…Tomato (Solanum lycopersicum L.) is a significant agricultural crop globally, serving as a vital source of food, income, and exports across many regions, including Europe, East Africa, and Indonesia [1,2]. Its economic importance is underscored by global production of 189.1 million t and cultivation on 5.1 million ha in 2021 [3], due to its widespread consumption and utilization in the food industry [4].…”

Section: Introductionmentioning

confidence: 99%

Biological Control of Pseudomonas syringae in Tomato Using Filtrates and Extracts Produced by Alternaria leptinellae

García-Latorre,

Rodrigo,

Santamaria

2024

Horticulturae

View full text Add to dashboard Cite

Endophytic fungi offer promising alternatives for sustainable plant disease management strategies, often through the production of bioactive secondary metabolites. This study investigated the biocontrol potential of filtrates and extracts, produced under controlled conditions, from Alternaria leptinellae E138 against Pseudomonas syringae in tomato plants under greenhouse conditions. To understand the main mechanisms involved in biocontrol, the direct inhibition of bacterial growth and disruption of quorum sensing activity caused by metabolites were studied in vitro, as well as indirect mechanisms, such as their capacity to produce phytohormone-like substances, nutrient mobilization, and antioxidant activity, which can enhance plant growth and fitness. Moreover, a mass spectrometry analysis was used to tentatively identify the secondary metabolites present in the extract with antimicrobial properties, which could explain the biocontrol effects observed. Mycopriming assays, involving the direct treatment of tomato seeds with the fungal A. leptinellae E138 extracts, produced increased germination rates and seedling vigor in tomato seeds. As another treatment, postemergence application of the extracts in greenhouse conditions significantly improved plant health and resulted in a 41% decrease in disease severity. Overall, this study underscores the potential of A. leptinellae E138 extract as a plant growth promoter with biocontrol capabilities, offering promising avenues for sustainable plant disease management.

show abstract

Establishing an inexpensive, space efficient colony of Bemisia tabaci MEAM1 utilizing modelling and feedback control principles

Cited by 6 publications

References 16 publications

Enabling biocontained plant virus transmission studies through establishment of an axenic whitefly (Bemisia tabaci) colony on plant tissue culture

Enabling biocontained plant virus transmission studies through establishment of an axenic whitefly (Bemisia tabaci) colony on plant tissue culture

Enabling biocontained plant virus transmission studies through establishment of axenic whitefly (Bemisia tabaci) colony on plant tissue culture

Biological Control of Pseudomonas syringae in Tomato Using Filtrates and Extracts Produced by Alternaria leptinellae

Contact Info

Product

Resources

About