Establishing cell lines for canine tonsillar and non-tonsillar oral squamous cell carcinoma and identifying characteristics associated with malignancy

Noguchi, Shunsuke; Hattori, Asuka; Tanimoto, Nanami; Nishida, Ruisa; Hirano, Kazuya; Wada, Yusuke; Matsuyama, Satoshi; Shimada, Terumasa; Akiyoshi, Hideo

doi:10.1016/j.tice.2020.101408

Cited by 7 publications

(11 citation statements)

References 11 publications

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“…10,11 Recently, we established several cell lines of canine oral SCC, and the expression of vimentin, a mesenchymal marker, was observed in canine oral SCC cells and tissues. 12,13 This finding indicates that EMT may be induced in canine oral SCC. Although SLUG has been suggested to induce the expression of vimentin in human cancer, 7 the functions of SLUG in canine cancer remain unknown.…”

Section: Introductionmentioning

confidence: 69%

“…Recently, we established several cell lines of canine oral SCC, and the expression of vimentin, a mesenchymal marker, was observed in canine oral SCC cells and tissues 12,13 . This finding indicates that EMT may be induced in canine oral SCC.…”

Section: Introductionmentioning

confidence: 80%

“…However, the mechanisms of invasion and metastasis have not yet been evaluated, while those have been elucidated well in human cancers. Recently, we established canine oral SCC cell lines, which made it possible to elucidate the mechanisms of malignancy in in vitro and in vivo experiments using xenografted mouse models 12 …”

Section: Discussionmentioning

confidence: 99%

“…Cell line validation statement: All of cell lines were previously established by the authors 12 . The cells were not subjected to species verification testing to investigate possible contamination including mycoplasma; however, the morphology under light microscopy and the growth kinetics remained consistent throughout the experiments.…”

Section: Methodsmentioning

confidence: 99%

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SLUG is upregulated and induces epithelial mesenchymal transition in canine oral squamous cell carcinoma

Noguchi

Hirano

Tanimoto

et al. 2021

Vet Comparative Oncology

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SLUG, encoded by the Snai2 gene, is known to play a role in epithelial-mesenchymal transition (EMT), which contributes to cell invasion and metastasis in some types of human carcinomas. However, the mechanisms and roles of EMT in canine squamous cell carcinoma (SCC) have not yet been elucidated. We have previously established canine oral SCC cell lines, including tonsillar SCC, and in this study, we evaluated the effects of SLUG on the phenotypes regarding EMT of canine SCC cells. First, immunohistochemical analysis revealed that SLUG is upregulated in canine oral SCC tissues compared to that in non-tumoural oral mucosa. Furthermore, gain-of-function and loss-of-function of SLUG revealed that SLUG partly contributed to migration and invasion of cells, as well as the upregulation of EMT markers such as vimentin and SNAIL. Thus, the current study suggests that SLUG promotes cell migration and invasion through EMT induction in canine oral SCC, as well as human cancers.

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Section: Introductionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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SLUG is upregulated and induces epithelial mesenchymal transition in canine oral squamous cell carcinoma

Noguchi

Hirano

Tanimoto

et al. 2021

Vet Comparative Oncology

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“…TSCC, identified as the most common type of tongue cancers, has a propensity of regional recurrence and lymphoid metastasis [ 23 , 24 ]. Though the standardized chemotherapy of cisplatin for TSCC patients would bring survival amelioration, the emergence of chemo-resistance is inevitable, ultimately leading to functional defects [ 24 , 25 ].…”

Section: Oncogenic Role Of Prr11 In Various Malignanciesmentioning

confidence: 99%

PRR11 in Malignancies: Biological Activities and Targeted Therapies

Han

Chen

2022

Biomolecules

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Proline rich 11 (PRR11), initially renowned for its relevance with cell-cycle progression, is a proline-rich protein coding gene in chromosome 17q22-23. Currently, accumulating studies have demonstrated that PRR11 plays a critical role in cellular proliferation, colony formation, migration, invasion, cell-cycle progression, apoptosis, autophagy and chemotherapy resistance via multiple signaling pathways and biological molecules in several solid tumors. In particular, PRR11 also serves as a promising prognostic indicator in a limited number of human cancers, gradually manifesting its potential application for targeted therapies. In this review, we summarize functional activities, related signaling pathways and biological molecules of PRR11 in various malignancies and generalize potential application of PRR11 for targeted therapies, thereby contributing to further exploration of PRR11 in cancer treatment.

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Interleukin-6 promotes the epithelial mesenchymal transition in canine tonsillar squamous cell carcinoma cells

Noguchi,

Shimonishi

2025

Research in Veterinary Science

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Establishing cell lines for canine tonsillar and non-tonsillar oral squamous cell carcinoma and identifying characteristics associated with malignancy

Cited by 7 publications

References 11 publications

SLUG is upregulated and induces epithelial mesenchymal transition in canine oral squamous cell carcinoma

SLUG is upregulated and induces epithelial mesenchymal transition in canine oral squamous cell carcinoma

PRR11 in Malignancies: Biological Activities and Targeted Therapies

Interleukin-6 promotes the epithelial mesenchymal transition in canine tonsillar squamous cell carcinoma cells

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