Establishment and Cryopreservation of a Giant Panda Skeletal Muscle-Derived Cell Line

Yu, Fang-Jian; Zeng, Changjun; Zhang, Yan; Wang, Chengdong; Xiong, Tie-Yi; Fang, Sheng‐Guo; Zhang, Hemin

doi:10.1089/bio.2014.0073

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…In our study, muscle cells obtained showed more than 90% viability after thawing and demonstrated relatively moderate population doubling times (52.98 h) compared to other muscle cell lines derived from Giant Panda (33.8 h) [40] and triploid olive founder (69.88 h) [41], respectively. Tese results indicated that WYSM cells were successfully cryopreserved and revived.…”

Section: Discussionmentioning

confidence: 55%

Germplasm Rescue of Postmortem Critically Endangered Yangtze Sturgeon (Acipenser dabryanus) by Cell Preservation

Liu

Wang

et al. 2023

Aquaculture Research

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With the growing sophistication of cloning technology, rescuing cell resources is of great significance for the protection of endangered animals. The Yangtze sturgeon (Acipenser dabryanus), one of the three Acipenseriformes species in the Yangtze River, is critically endangered. Natural reproduction of the Yangtze sturgeon has not been detected since 2000. Less than 20 wild individuals are kept in husbandry, and all are too old to breed. Therefore, it is urgent to rescue the genetic resources of every wild Yangtze sturgeon. Here, we isolated and preserved viable cells from critically endangered postmortem Yangtze sturgeon for the first time. Attempts to rescue and preserve cell resources were carried out from 8 tissues, brain, kidney, gonad, fin, liver, skin, spleen, and muscle of an over 35-year-old female wild Yangtze sturgeon between 11 and 14 hours after death at 19.8°C in an outdoor concrete pond, and only muscle tissue cells could be successfully subcultured and preserved. Furthermore, the cultured cells showed high post-thaw cell viability and normal growth with a population doubling time of 52.98 h. Moreover, they were fibronectin- and desmin-positive, characterizing them as fibroblastic confirmed muscle cells with fibroblastic properties and myogenic origin. Tests for microbial contamination of the cell lines were negative. Chromosome analysis demonstrated that muscle cells possess a modal polyploid chromosome number of 264. The mitochondrial sequence data of COI genes and 12S rRNA confirmed that the developed cell line originated from A. dabryanus. Furthermore, transfection results indicate that muscle cells could be used for gene manipulation and functional studies. These results suggest that viable muscle cells could also be successfully isolated and cryopreserved from the wild Yangtze sturgeon in a short time after death. This report is not only of great significance for the germplasm rescue of critically endangered Yangtze sturgeon but also provides some scientific reference for the germplasm preservation of other endangered fish.

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Section: Discussionmentioning

confidence: 55%

Germplasm Rescue of Postmortem Critically Endangered Yangtze Sturgeon (Acipenser dabryanus) by Cell Preservation

Liu

Wang

et al. 2023

Aquaculture Research

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“…Cryopreservation of cell lines plays an important role in preserving the genetic resources of threatened species (Cetinkaya et al 2011). In our study, the obtained muscle cells showed more than 90% viability after post-thaw and demonstrated a relatively moderate population doubling times (52.98h) compared to other muscle cell lines, derived from Giant Panda (33.8h) (Yu et al 2015) and triploid olive ounder (69.88h) (Peng et al 2016), respectively. These results indicated that the WYSM cells have been successfully cryopreserved and revived.…”

Section: Discussionmentioning

confidence: 56%

Germplasm rescue of post-mortem critically endangered Yangtze Sturgeon by cell cryopreservation

Liu

Wang

et al. 2022

Preprint

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With the growing sophistication of cloning technology, rescuing cell resources is of great significance for the protection of endangered animals. The Yangtze sturgeon (Acipenser dabryanus), one of the three Acipenseriformes species in the Yangtze River, is critically endangered. Natural reproduction of the Yangtze sturgeon has not been detected since 2000. Less than 20 wild individuals are kept in husbandry and all are too old to breed. Therefore, it is urgently to rescue the genetic resources of every wild Yangtze sturgeon. Here, we isolated and preserved viable cells from the post-mortem critically endangered Yangtze Sturgeon for the first time. Attempt of rescuing and preserving cell resources were carried out from 8 tissues, brain, kidney, heart, fin, liver, skin, spleen and muscle of over-35-years-old female wild Yangtze sturgeon between 11 and 14 hours after death at 19.8℃ in outdoor concrete pond, and only muscle tissue cells could be successfully sub-cultured and preserved. Furthermore, the cultured cells were assessed by population doubling time, immunofluorescence analysis, microorganism detection, karyotyping and origin identification, and no abnormality was observed in the morphology, structure, growth and ploidy. These results suggest that viable cells could also be successfully isolated and cryopreserved from the wild Yangtze sturgeon in a short time after death, and muscle tissues had more potential to separate living cells than other tissues. This report is not only of great significance to the germplasm rescue of critically endangered Yangtze sturgeon, but also provides some scientific reference for the germplasm preservation of other endangered fish.

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“…This bank preserves plant seeds of 10,000 species, animal germplasms, macrofungi, and microorganisms. In addition, Chinese academic institutes now explore the cryopreservation option for cell lines of wild animals, such as Bengal tigers (Guan et al 2010 ) and pandas (Yu et al 2015 ).…”

Section: Biobanks By Countriesmentioning

confidence: 99%

Publicly-funded biobanks and networks in East Asia

2016

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With the enactment of the Nagoya Protocol, international competitions to secure biological resources are intensifying. Biobanking is one of the many attempts to preserve biological resources and their information for the use in future research and development. Asian countries, especially China, Japan, and Korea are very active in biobanking activities under the strategic plans coordinated by their governments. They also proactively established networks for biobanks of Asia to facilitate resource and expertise sharing. Biobanks of these countries should furthermore standardize operating procedures and diversify funding sources for establishing stable operation systems.

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Establishment and Cryopreservation of a Giant Panda Skeletal Muscle-Derived Cell Line

Cited by 6 publications

References 15 publications

Germplasm Rescue of Postmortem Critically Endangered Yangtze Sturgeon (Acipenser dabryanus) by Cell Preservation

Germplasm Rescue of Postmortem Critically Endangered Yangtze Sturgeon (Acipenser dabryanus) by Cell Preservation

Germplasm rescue of post-mortem critically endangered Yangtze Sturgeon by cell cryopreservation

Publicly-funded biobanks and networks in East Asia

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