Establishment of a cDNA library from Bambusa edulis Murno in vitro-grown shoots

Abstract

An expressed sequence tag (EST) analysis approach was undertaken to identify the genes involved in photosynthesis and metabolism of Bambusa edulis Murno, an important bamboo crop species in Asia. The cDNA library was constructed from in vitro-grown multiple shoots. Sequencing of the cDNA clones generated 987 5 0 -end high-quality expressed sequence tags (ESTs, Accession number: FG551848-FG552834), of which 792 were revealed by sequence comparison to be unique. A BLASTX analysis showed that 645 of these genes were similar to genes present in the NCBI database. A total of 389 genes were most similar to rice genes. Nine genes had an E-value of zero. Functional classification of these genes by the Munich Information Center for Protein Sequences (MIPS) showed that most of the genes were related to metabolism and subcellular localization processes. PSI assembly protein (Beycf3) expression was absent in the albino mutant, while PSII 43-kDa protein (BepsbC), chlorophyll synthase (BeCAO) and PSII 10-kDa protein (BePsbR) expression were reduced. These data suggest that in this chloroplast genome-aberrated mutant, both chloroplast-encoded and nucleusencoded genes were repressed and that this library will be useful for further investigation of bamboo.

Understanding bamboo flowering based on large-scale analysis of expressed sequence tags

“…When two genes, DlMADS8 and DlMADS18, cloned from young spikelets of a bamboo species (Dendrocalamus latiflorus) were overexpressed in Arabidopsis, curled leaves and early flowering was exhibited (Tian et al, 2005(Tian et al, , 2006. Recently, a cDNA library from the in vitro grown shoots of a bamboo species (Bambusa edulis) was established and 987 ESTs (expressed sequence tags) were identified, but no flowering-related ESTs were specified (Liu et al, 2008).…”

An Expressed Sequence Tag Analysis for the Fast-Growing Shoots of Bambusa edulis Murno

“…Finally, functional categories were defined using the Gene Ontology (GO) Database (www.geneontology.org) and the BLASTX algorithm. To compare our data and the previous ESTs from B. edulis Murno in vitro-grown multiple shoots (Liu et al 2008), we downloaded a dataset from the National Center for Biotechnology (NCBI) database (FG551848-FG552834) to our local system. Afterwards, sequence assembly was carried out using the combined dataset of previous ESTs and our ESTs to find libraryspecific or common unigenes.…”

“…Bamboo is one of the tallest species in the Poaceae and is one of the fastest growing plants in the world (Liu et al 2008).…”

“…Genes encoding sucrose synthase, invertase, and cellulose synthase have been isolated and characterized from various bamboo species (Chiu et al 2006;Hsieh et al 2006;Chen et al 2010). Expressed sequence tag (EST) analysis was performed with in vitro-grown Bambusa edulis Murno shoots to identify the genes involved in photosynthesis (Liu et al 2008) and with samples of various tissues to construct a full-length cDNA database (Peng et al 2010). Microarray analysis was conducted of an albino mutant of bamboo ).…”

Isolation and Partial Characterization of an R2R3MYB Transcription Factor from the Bamboo Species Fargesia fungosa

“…Until the recent publication of EST sets of Phyllostachys heterocycla (Moso bamboo; Peng et al 2010) and Bambusa (Liu et al 2008;Lin et al 2010), bamboo was the only large subfamily of Poaceae with little genomic information available, and there is still a paucity of functional molecular information for this key group of species. We report here the isolation from Fargesia fungosa of a member of the R2R3MYB transcription factor family with strong similarity to genes demonstrated to regulate lignification in other plant species.…”