Establishment of a csf‐producing cell line from a human gastric carcinoma and characteristics of the csf produced by that cell line

Abstract

A human cell line producing colony-stimulating factor has been established in vitro from a human gastric carcinoma. The cell line was transplantable into nude mice which developed a marked neutrophilia. The cell line has been maintained for three years. The cells grew in a monolayered sheet and produced colony-stimulating factors that enhanced the formation of granulocyte and monocyte colonies in vitro with mouse bone marrow cells as the target and granulocyte colonies with human bone marrow cells as the target.

Establishment and characterization of a cell line (IGSK-3) secreting human chorionic gonadotropin, adrenocorticotropic hormone and parathyroid hormone-related protein derived from primary poorly differentiated adenocarcinoma of the stomach

“…There are many reports on the establishment of cell lines derived from original, metastatic foci or recurrent gastric tumors, 1–16 including the TMK‐1, 1 and MKN‐45 cell lines derived from poorly differentiated adenocarcinoma, 2 MKN‐1 derived from adenosquamous carcinoma, 2 MKN‐7 and MKN‐28 cell lines derived from well differentiated adenocarcinoma, MKN‐74 derived from moderately differentiated tubular adenocarcinoma, 2 KATO‐III and HSC‐39 derived from signet ring cell carcinoma, 3,4 and HSC‐44 PE and HSC‐58 derived from scirrhous carcinoma 16 …”

Establishment and characterization of a cisplatin-resistant cell line (IGSK-1) from a poorly differentiated gastric adenocarcinoma

“…The chromosome number is shown in Table 1. G-band karyotypes of IGSK-1 were 77-85 [4n], XXX, −X[10], −1 [10], add(1)(p11) [9], −2 [4], −4 [10], del(4)(q?) [5], −5 [3], add(6)(q13) [8], +7 [7], add(8)(p11) [10], add(8)(p21) [8], inv(9)(p11q13) × 2 [10], −10 [10], +13 [2], −13 [6], −14 [10], −14 [4], −15 [10], −16 [10], add(16)(p13) × 2 [10], Journal compilation © 2007 Japan Human Cell Society add(16)(p13) [10], −17 [4], add(17)(q21) [10], add(16)(p13) [10], +18 [8], +18 [3], add(18)(q21) [8], −19 [9], add(19)(p13) [2], −20 [10], −20 [7], −21 [10], −22 [10], +mar1 [9], +0-4mar, 1-15dmin as shown in Fig.…”

“…There are many reports on the establishment of cell lines derived from original, metastatic foci or recurrent gastric tumors, [1][2][3][4][5][6][7][8][9][10][11][12][13] including: the TMK-1 and MKN-45 cell lines derived from poorly differentiated adenocarcinoma; MKN-1 derived from adenosquamous carcinoma; MKN-7 and MKN-28 cell lines derived from well differentiated adenocarcinoma, MKN-74 derived from moderately differentiated tubular adenocarcinoma; KATO-III and HSC-39 derived from signet ring cell carcinoma; 12 and HSC-44 PE and HSC-58 derived from scirrhous carcinoma. 13 However, this is the first report of the establishment of a gastrin and serotonin secreting and serotonin incorporating cell line derived from gastric poorly differentiated adenocarcinoma.…”

Establishment and characterization of an IGSK-2 cell line derived from ascitic fluid of recurrent hCG and somatostatin secreted adenocarcinoma of the stomach

“…There are several reports on the establishment of cell lines derived from stomach cancer 1–11 . As stomach cancer is a relatively common disease in Japan, the chemotherapeutic approach is very common and useful.…”