Establishment of a n  efficient method for kiwifruit  in vitro  micrografting

Abstract

This study was carried out to assess the potential use and applicability of micrografting technique for developing in vitro grafted plantlets. Microshoots of kiwifruit ‘Miliang-1’ and ‘Hongyang’ was used as rootstock and scion. Accomplishment of in vitro grafting has been examined by varying numerous factors, the physiological state of the rootstock and scion, the pH value of the medium, the concentration of sucrose, the type of medium and the PGRs. The results showed that the best condition of kiwifruit in vitro grafting was that the rootstock does not take root, and both the rootstock and scion have no leaves. The best medium formula suitable for kiwifruit micrografting was 1/2 MS solid media along with 0.5 mg L− 1 GA3, 1.0mg L− 1 IBA, 40 g L− 1 sucrose and 7 g L− 1 agar, pH 6.0. The matrix formula of perlite: peat soil: vermiculite volume ratio of 1:2:1 was the most suitable substrate for transplanting micrografted seedlings.