Establishment of a relationship between grapevine leafroll closteroviruses 1 and 3 by use of monoclonal antibodies

Seddas, A.; Haidar, Malak; Greif, Charles; Jacquet, Christophe; Cloquemin, Gérard; Walter, Bernard

doi:10.1046/j.1365-3059.2000.00415.x

Cited by 21 publications

(5 citation statements)

References 22 publications

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“…In addition to the immunological method of enzyme‐linked immunosorbent assay (ELISA) (Seddas et al. ) and the molecular method of reverse transcription polymerase chain reaction (RT‐PCR) (Ling et al. , Osman et al.…”

Section: Introductionmentioning

confidence: 99%

Abiotic stress improves in vitro biological indexing ofGrapevine leafroll-associated virus-3in red grapevine cultivars

Cui

et al. 2015

Australian Journal of Grape and Wine Research

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Background and Aims:Virus detection is necessary for in vitro production and propagation of virus-free plants, for in vitro conservation and when material is received from overseas in tissue culture. In vitro biological virus indexing is among the reliable techniques. The aim of the present study was to develop an in vitro graft-free protocol for indexing of Grapevine leafroll-associated virus-3 (GLRaV-3). Methods and Results: In vitro GLRaV-3-infected shoots were cultured on half-strength Murashige and Skoog medium supplemented with either 150 mmol NaCl or 4% polyethylene glycol (PEG) 8000 to induce development of the virus symptoms. After 4 weeks of culture, the proportion of the symptom expression was 86 and 81% for Cabernet Sauvignon, 100 for '6-12-2', 78 and 72% for Red Globe and 70 and 60% for Kyoho in the diseased shoots stressed by 150 mmol NaCl and 4% PEG, respectively. Expression of virus symptoms was more obvious and reliable when in vitro plantlets were stressed by NaCl than by PEG. Conclusions: Salt and drought stress induced by NaCl and PEG 8000 can improve in vitro biological indexing of GLRaV-3 in red grapevine cultivars. Significance of the Study: The techniques developed in the present study would have potential application to GLRaV-3 indexing of red grapevine cultivars.

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Section: Introductionmentioning

confidence: 99%

Abiotic stress improves in vitro biological indexing ofGrapevine leafroll-associated virus-3in red grapevine cultivars

Cui

et al. 2015

Australian Journal of Grape and Wine Research

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“…Other ORFs encodes in the 5¢-3¢ direction a small hydrophobic protein, a homologue of the HSP7h family of heat shock proteins, a HSP9h-like protein, capsid protein (CP), two diverged copies of the CP (CPd1 and CPd2) and two other proteins of unknown function [10]. Viral RNA is encapsidated in filamentous particles containing a coat protein with a Mr of 39 kDa [11].…”

Section: Introductionmentioning

confidence: 99%

Analysis of the Molecular Variability of Grapevine Leafroll-associated Virus 1 Reveals the Presence of Two Distinct Virus Groups and their Mixed Occurrence in Grapevines

2005

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Genetic diversity of eight Grapevine leafroll-associated virus 1 (GLRaV-1) isolates recovered from grapevines in three distinct locations in the Czech Republic and Slovakia was characterised by restriction fragment length polymorphism (RFLP) analysis and by sequencing of cloned 540 bp fragment of the heat shock protein 70 (HSP70) gene. Comparison and phylogenetic analysis of obtained and previously available sequence data revealed the existence of two groups of GLRaV-l isolates, tentatively named A and E (genetic divergence between A and E group reached 13.9%). An RT-PCR detection method followed by simple restriction analysis was developed, showing the potential to differentiate GLRaV-1 isolates of these groups. Interestingly, a mixed infection of two GLRaV-1 groups in the same plant was frequently detected together with a high intra-group variability in some isolates.

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“…Özellikle de bulaşık soysuzlaşma hastalığına neden olan virüslerden bazıları (ArMV-GFLV; TBRV (subgroup B)-GCMV; GDefV-ArMV; BLMoV-GBLV) serolojik olarak, bazıları da filogenetik olarak (GARSV, GCMV ve TBRV) [49,27,54], yaprak kıvrılma hastalığına yol açan virüslerden GLRaV-1 ve GLRaV-3 [70]; rugoz odun hastalığına yol açan virüslerden GVA-GVB, GVA-GVD birbirleriyle serolojik olarak ilişkili [39,19] olduğundan bu virüslerin teşhisinde antiserum kalitesi belirleyici bir etken olmuştur. Bununla birlikte yaprak özütleri bütün odunsu bitkilerden çok daha fazla inhibitör maddeler içerdiğinden serolojik çalışmalarda antiserum seçiminin yanında izolasyon yöntemleri de bu dönemde virüslerin teşhisinde belirleyici rol oynamışlardır.…”

Section: Türkiye'de Asma Virüs çAlışmalarının Tarihsel Gelişimi Ve Karşılaşılan Sorunlarunclassified

Türkiye’de Belirlenen Bağ Virüs Hastalıklarının Tarihsel Gelişimi ve Önemi

Akbaş¹,

Morca²,

Coşkan³

2021

Bahçe

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Türkiye bağcılığı sosyoekonomik anlamda en önemli ürün gruplarının arasında yer almaktadır. Bağlarda birçok zararlı organizma grubu özellikle de virüs enfeksiyonları bitkiyi zayıflatıp, verim ve kalitesinde azalmalara yol açtığından karantina ve sertifikasyonda başı çekmektedir. Son yarım asırda ülke bağ virüs araştırmalarında önemli çalışmalar yapılmış ve özellikle son 20 yılda virüslerin tanımı, karakterizasyonu ve mücadele yöntemleri alanında önemli ilerlemeler kat edilmiştir. Türkiye bağ yetiştiriciliği yapılan alanlarında şu ana kadar viral hastalıklara neden olan yaklaşık 23 virüs ve 5 viroidin varlığı belirlenmiştir. Arabis Mosaic Virüs (ArMV), Grapevine Fanleaf Virüs (GFLV), Grapevine Fleck Virüs (GFkV), Grapevine Leafroll-Associated Viruses (GLRaV-1, GLRaV-3) ve Grapevine Virüs A (GVA) en yaygın bağ virüsleri arasında yer almaktadır. Grapevine Virüs B (GVB), Grapevine Pinot Gris Virüs (GPGV), Grapevine Roditis Leaf Discoloration-Associated Virüs (GRLDaV), Grapevine Rupestris Stem Pitting-Associated Virüs (GRSPaV) ve Grapevine Syrah Virüs 1 (GSyV-1) gibi önemli viral etmenler de ülkede rapor edilmiştir. Türkiye’de bağ virüslerinin teşhisinde otsu-odunsu konukçulara indeksleme ve serolojik testler gibi geleneksel analiz yöntemlerinin yanında, moleküler analiz yöntemleri ve son dönemde yeni nesil dizileme teknolojisi (Next Generation Sequencing-NGS) gibi DNA dizileme tekniklerinin kullanımı ile teşhis ve karakterizasyon çalışmaları oldukça hızlanmıştır. Bu derlemede Türkiye’de bağ virüslerinin tarihsel gelişimi, oluşturduğu hastalıklar ve ekonomik önemi hakkında kısa bilgilere yer verilmiştir.

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Establishment of a relationship between grapevine leafroll closteroviruses 1 and 3 by use of monoclonal antibodies

Cited by 21 publications

References 22 publications

Abiotic stress improves in vitro biological indexing ofGrapevine leafroll-associated virus-3in red grapevine cultivars

Abiotic stress improves in vitro biological indexing ofGrapevine leafroll-associated virus-3in red grapevine cultivars

Analysis of the Molecular Variability of Grapevine Leafroll-associated Virus 1 Reveals the Presence of Two Distinct Virus Groups and their Mixed Occurrence in Grapevines

Türkiye’de Belirlenen Bağ Virüs Hastalıklarının Tarihsel Gelişimi ve Önemi

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