Establishment of an efficient genetic transformation system: A case study of RNAi-induced silencing of the transcription factor MeGI in Diospyros oleifera Cheng seedlings

Mai, Yini; Liu, Yang; Yuan, Jiaying; Ye, Lingshuai; Zhang, Yue; Han, Weijuan; Suo, Yujing; Li, Huawei; Hu, Ruiyang; Sun, Peng; Li, Ze; Fu, Jianmin

doi:10.1016/j.scienta.2022.111560

Cited by 2 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fruits were brought to the laboratory at room temperature, and the immature seeds were removed from the fruit, washed with water, shade-dried, and stored in a plastic bag at 4 • C in a refrigerator. D. oleifera as a rootstock has root-deep drought tolerance, strong growth potential, early results, and a dwarf tree shape [10].…”

Section: Plant Materialsmentioning

confidence: 99%

“…The establishment of a D. oleifera tissue culture and leaf regeneration system would not only accelerate seedling growth, but also enable molecular breeding work. Because the genes that control the flower sex in D. oleifera have been identified and have significant economic values, it is urgent to establish a genetic transformation system based on tissue culture [10].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

Liu,

Zhou,

Luo

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

Persimmons (Diospyros) are economically important trees that are widely cultivated for wood production in China, and Diospyros oleifera Cheng is the main persimmon grafting stock. However, an efficient tissue culture system has not been perfected for D. oleifera due to the limits of proliferation and rooting cultures. Therefore, this study examined the effects of different plant growth regulators and concentrations on the primary culture of young embryos, induction of leaf callus, differentiation of adventitious shoots, and rooting culture of D. oleifera. The optimal formula for young embryo germination was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg/L gibberellic acid (GA3); after 25 days, the sprouting rate of the young embryos was 67.3%. The best medium for leaf callus induction was 1/2MS medium containing 2.0 mg/L of 6-benzylaminopurine (6-BA) and 0.5 mg/L of naphthaleneacetic acid (NAA), and the callus induction rate was 88.9%. Then, the callus was transferred to 1/2MS medium containing 2.0 mg/L of zeatin (ZT), 0.5 mg/L of NAA, and 2.0 mg/L of thidiazuron (TDZ) to induce adventitious shoots; after 25 days, 5.4 buds were produced per explant, and the induction rate of the adventitious shoots was 88.3%. The adventitious shoots were transferred to 1/2MS medium containing 2.0 mg/L of ZT, 2.0 mg/L of 6-(γ,γ-dimethylallylamino)purine (2iP), and 0.1 mg/L of indole acetic acid (IAA) for the proliferation culture, for which the multiplication coefficient approached 7.5. After multiplication, the adventitious shoots were inoculated into 1/2MS medium containing 1.0 mg/L of indole butyric acid (IBA), 0.5 mg/L of NAA, and 1.0 mg/L of kinetin (KT); the rooting rate was 60.2%, and the average number of roots was 6.9.

show abstract

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

Liu,

Zhou,

Luo

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genetic transformation of plants is important in crop improvement and breeding and is widely used in the breeding of forest trees. However, efficient transformation and regeneration remain major problems for the breeding of many plants [12][13][14]. Therefore, there is an urgent need to develop an efficient sagrobacterium-mediated transformation scheme for F. mandshurica that can produce a large number of transgenic plants in a short time.…”

Section: Introductionmentioning

confidence: 99%

A Stable and Efficient Genetic Transformation Method for Embryogenic Callus of Fraxinus mandshurica

Dong

Zhao

et al. 2023

Forests

View full text Add to dashboard Cite

Fraxinus mandshurica is a widely used greening and ornamental tree species. However, its genetic transformation system has been hampered by problems such as low transformation efficiency, among others, which can hinder research related to molecular breeding and the analysis of functional genes. Thus, in this study, a novel genetic transformation method for efficient transformation of the embryonic callus of Fraxinus mandshurica was investigated. The method was optimized in terms of factors such as antibiotics, infection solution concentrations, co-culture time, and somatic embryo maturation. The results indicated that the optimal antibiotic concentration was 10 mg·L−1 of hygromycin (Hyg). At this point, the callus proliferation multiple was only 0.12. The highest transformation efficiency was found to be 93.93% when the absorbance of the infection solution concentration at OD600 was 0.4. Interestingly, transformation efficiency was found to be highest (77.9%) at 48 h of co-culture, with a GUS staining rate of 88.23%. The medium for somatic embryo maturation of transformed callus was half-strength MS medium (MS 1/2) containing 60 g·L−1 polyethylene glycol, 1 mg·L−1 abscisic acid, 400 mg·L−1 casein enzymatic hydrolysate (CH), 20 g·L−1 sucrose, 1 g·L−1 activated charcoal, and 5 g·L−1 gellan gum. The medium for somatic embryo germination was MS ½, containing 0.2 mg·L−1 of N-(Phenylmethyl)-9H-purin-6-amine(6-BA) and 5.0 mg·L−1 of gibberellin (GA). These results are of significance for the verification of the gene function and future genetic improvement of Fraxinus mandshurica.

show abstract

Establishment of an efficient genetic transformation system: A case study of RNAi-induced silencing of the transcription factor MeGI in Diospyros oleifera Cheng seedlings

Cited by 2 publications

References 28 publications

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

A Stable and Efficient Genetic Transformation Method for Embryogenic Callus of Fraxinus mandshurica

Contact Info

Product

Resources

About