Establishment of cell lines stably expressing HNA‐1a, ‐1b, and ‐2a antigen with low background reactivity in flow cytometric analysis

Yasui, Kazuta; Miyazaki, Tôru; Matsuyama, Nobuki; Kojima, Yoshitaka; Furuta, Rie; Fujisawa, Jun–ichi; Tani, Yoshihiko; Shibata, Hirotoshi; Sato, Sunao; Kato, Toshiaki; Ikeda, Hisami; Hirayama, Fumiya

doi:10.1111/j.1537-2995.2006.01139.x

Cited by 36 publications

(46 citation statements)

References 39 publications

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“…1. KY-HNA-1a cells were established by transducing the HNA-1a gene with a retrovirus vector to confer stable transgene expression in K562 cells that exhibited low background reactivity to human serum samples obtained from healthy donors, as described earlier [7]. The KY-MAIGA system for HNA-1a Abs detection based on KY-HNA-1a panel whole blood of HNA-1a/a was used as a neutrophil source.…”

Section: Serum Samplesmentioning

confidence: 99%

“…However, conventional neutrophil-based tests have two further drawbacks: firstly, a typed panel of neutrophils is required; secondly, the presence of other leukocyte antibodies, such as human leukocyte antigen (HLA) Abs, may affect the detection of HNA Abs. To overcome these drawbacks, we developed panel cell lines (so-called KY cells) capable of stably expressing HNA antigens and established KY cell-based IFT (KY-IFT) [7][8][9]. We have been using these two detection systems in combination to detect HNA Abs in transfusion reaction cases and neutropenia patients for several years.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of HNA-expressing cell line-based antigen capture systems and a solid-phase system for detecting HNA-1a antibodies

et al. 2015

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Granulocyte immunofluorescence and granulocyte agglutination tests are standard methods for detecting human neutrophil antigen (HNA) antibodies (Abs); however, these require a typed panel of neutrophils, which can be time-consuming to develop, and it remains difficult to determine antibody specificity in some cases. We established and evaluated four detection systems for HNA-1a Abs based on an HNA-1a-expressing cell line (KY cells) and antigen capture. We additionally evaluated a commercial solid-phase system. Eleven HNA-1a antibody-positive samples, including the World Health Organization Reference Reagent, and 40 serum samples derived from male blood donors were used as positive and negative control samples, respectively. Although specificity was >0.90 in all systems evaluated, the sensitivity varied among the systems. The KY cell-based monoclonal antibody specific immobilisation of granulocyte antigens (KY-MAIGA) system using certain, but not all, monoclonal Abs, and the solid-phase system revealed higher sensitivity than other systems. In conclusion, the KY-MAIGA and commercial solid-phase systems were superior in terms of specific and sensitive detection of HNA-1a Abs.

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Section: Serum Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of HNA-expressing cell line-based antigen capture systems and a solid-phase system for detecting HNA-1a antibodies

et al. 2015

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“…Recently, Yasui et al [4] reported the establishment of gene transfected K562 cell lines that express HNA-1a, -1b, or -2a antigens. Later on, they established new cell lines that selectively express HNA-1c, -4a, -4b, -5a, or -5b antigens [5].…”

Section: Discussionmentioning

confidence: 99%

Granulocyte antibody detection – the role of MPHA

Tsuno

Matsuhashi

Takahashi

2011

ISBT Science Series

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Human neutrophil antigens (HNA) are involved in the pathogenesis of a variety of clinical conditions, such as neonatal immune neutropenia (NIN), refractoriness to granulocyte transfusions, alloimune neutropenia after bone marrow transplantation, febrile transfusion reactions, and transfusion-related acute lung injury (TRALI). The accurate detection of granulocyte antibodies is essential for the diagnosis and prevention of the clinical conditions in which these antibodies are involved, especially TRALI. The international granulocyte immunology workshop recommends the use of granulocyte agglutination test (GAT) and granulocyte immunofluorescence test (GIFT) for the screening of granulocyte antibody, and the use of monoclonal antibody-specific immobilization of granulocyte antigen (MAIGA) assay for the determination of the antibody specificity. Presently, however, no single technique available is enough to detect all clinically relevant granulocyte antibodies. Here, we describe the advantages and disadvantages of the mixed-passive hemagglutination (MPHA) assay in the general context of granulocyte serology.

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“…Currently MAIGA is a laborious and time-consuming test, not suitable for antibody screening and rarely used in routine practice. Moreover it seems that GIFT is more sensitive than MAIGA [Bruin et al 2005], and consequently since new techniques such as mixed passive hemagglutination [Han et al 2006], transfected cell lines expressing neutrophil antigens [Yasui et al 2007[Yasui et al , 2008Woźniak et al 2012], soluble recombinant HNA production by transfection of insect cells [Bayat et al 2009;Werth et al 2013] or the microbead test [Fromont et al 2010] have not yet definitely proved their efficacy or are not yet commercially available, the majority of laboratories worldwide analyse patient sera by the flow cytometry assay.…”

Section: Diagnosismentioning

confidence: 99%

Diagnosis and management of primary autoimmune neutropenia in children: insights for clinicians

Farruggia¹,

Dufour

2014

Therapeutic Advances in Hematology

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Autoimmune neutropenia of infancy (AIN), also called primary autoimmune neutropenia, is a disease in which antibodies recognize membrane antigens of neutrophils, mostly located on immunoglobulin G (IgG) Fc receptor type 3b (FcγIIIb receptor), causing their peripheral destruction. It is the most frequent type of neutropenia in children under 3-4 years of age and in most cases shows a benign, self-limited course. The diagnosis is based on evidence of indirect antineutrophil antibodies, whose detection frequently remains difficult. In this review we have analyzed the literature regarding AIN and present our personal experience in diagnosis and management.

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Establishment of cell lines stably expressing HNA‐1a, ‐1b, and ‐2a antigen with low background reactivity in flow cytometric analysis

Abstract: These results indicate that the K562 panel cell lines provide a good panel for detecting HNA-reactive neutrophil antibodies in human serum samples.

Cited by 36 publications

References 39 publications

Evaluation of HNA-expressing cell line-based antigen capture systems and a solid-phase system for detecting HNA-1a antibodies

Evaluation of HNA-expressing cell line-based antigen capture systems and a solid-phase system for detecting HNA-1a antibodies

Granulocyte antibody detection – the role of MPHA

Diagnosis and management of primary autoimmune neutropenia in children: insights for clinicians

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