Establishment of different challenge models for Aeromonas salmonicida subsp. achromogenes in turbot and sole

YolandaTorres-Corral,; González-Barreiro, Oscar; Riaza, A.; Santos, Ysabel

doi:10.1016/j.aquaculture.2022.738261

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…Intraperitoneal injection is generally preferred in RSIV challenge experiments owing to its high rate of infection and the lower amount of virus required. However, this route of infection is considered unnatural because the virus bypasses the host’s mucosal immune barrier, which serves as the first line of defense [ 17 , 18 ]. In contrast, the immersion challenge represents a more natural route of infection and is, therefore, a viable alternative for studying virus entry and the temporal progression of the disease.…”

Section: Discussionmentioning

confidence: 99%

Impact of Red Sea Bream Iridovirus Infection on Rock Bream (Oplegnathus fasciatus) and Other Fish Species: A Study of Horizontal Transmission

Kim

Kang

Woo

et al. 2023

Animals

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Red sea bream iridovirus (RSIV) causes significant economic losses in aquaculture. Here, we analyzed the pathogenicity, viral shedding, and transmission dynamics of RSIV in rock bream (Oplegnathus fasciatus) by employing immersion infection and cohabitation challenge models. Rock bream challenged by immersion exposure exhibited 100% mortality within 35 days post RSIV exposure, indicating that the viral shedding in seawater peaked after mortality. At 25 °C, a positive correlation between the viral loads within infected rock bream and virus shedding into the seawater was observed. Specific RSIV lesions were observed in the spleen and kidney of the infected rock bream, and the viral load in the spleen had the highest correlation with the histopathological grade. A cohabitation challenge mimicking the natural transmission conditions was performed to assess the virus transmission and determine the pathogenicity and viral load. The RSIV-infected rock breams (donors) were cohabited with uninfected rock bream, red sea bream (Pagrus major), and flathead grey mullet (Mugil cephalus) (recipients) at both 25 °C and 15 °C. In the cohabitation challenge group maintained at 15 °C, no mortality was observed across all experimental groups. However, RSIV was detected in both seawater and the recipient fish. Our results provide preliminary data for further epidemiological analyses and aid in the development of preventive measures and management of RSIVD in aquaculture.

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Section: Discussionmentioning

confidence: 99%