Esterification of fatty acids using nylon-immobilized lipase in n-hexane: kinetic parameters and chain-length effects

Biotechnological Utilisation of Fusel Oil for Biolubricant Production

“…Thus the model seems too complicated having high uncertainty. Esterification of butyl alcohol by Candida rugosa lipase was studied by (Zaidi et al, 2002), where ping-pong bi-bi mechanism was assumed in the kinetical model with 5 parameters. However, the range of substrate concentration measured was quite narrow (0.1 -1 mol/L), and the error of the modelling was found very high (28 %).…”

“…Candida antarctica ordered bi-bi 13 parameters both S and P competitive (Garcia et al, 1996) too many parameters fitted to one measurement; high error of fitting butyl alcohol immobilised Candida rugosa ping-pong bi-bi 5 parameters both acid and alcohol (Zaidi et al, 2002) error is 28 %; narrow range of substrate concentration (0. Immobilized Rhizomucor miehei lipase was applied for ethyl oleate synthesis by (Oliveira et al, 2001).…”

“…Soluble porcine pancreatic lipase was applied for the ethyl oleate synthesis by (Hazarika et al, 2002). They assumed ping-pong mechanism, as well, containing 4 parameters, however the range of substrate concentration studied was even narrower (0.3 -0.8 mol/L) than in case of (Zaidi et al, 2002). Description of the correct kinetics on the particular esterification reaction is even more difficult due to the various possible inhibition effects.…”

“…Esters prepared from the reaction of long-chain acids with long-chain alcohols (12-20 carbon atoms) also have important applications as plasticizers and lubricants (Zaidi et al, 2002, Dossat et al, 2002. The direct effect of the ester group on the physical properties of a lubricant is to lower the volatility and raise the flash point.…”

Kinetic Behaviour of Free Lipase and Mica-Based Immobilized Lipase Catalyzing the Synthesis of Sugar Esters

“…8) The most generally accepted method for the lipasecatalyzed reaction of fatty acid esters involves the PingPong Bi-Bi mechanism. [9][10][11][12][13][14] However, the kinetic investigation of sugar ester synthesis catalyzed by lipase immobilized on a natural support is still rare. To the best of our knowledge, the use of natural mica as a lipase support in the coupling reaction of lactose sugar with capric acid has not been exploited so far in a kinetic study.…”