2021
DOI: 10.1101/2021.05.24.445502
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Estimating the Dynamic Range of Quantitative Single-Molecule Localization Microscopy

Abstract: In recent years, there have been significant advances in quantifying molecule copy number and protein stoichiometry with single-molecule localization microscopy (SMLM). However, as the density of fluorophores per diffraction-limited spot increases, distinguishing between detection events from different fluorophores becomes progressively more difficult, affecting the accuracy of such measurements. Although essential to the design of quantitative experiments, the dynamic range of SMLM counting techniques has not… Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2021
2021
2021
2021

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(1 citation statement)
references
References 43 publications
0
1
0
Order By: Relevance
“…Eq. 2 was derived from the results presented in ( Nino and Milstein, 2021 ) by assuming k on ≪ k off with no restriction on k bleach and by accounting for p miss . Similarly, the cumulative number of preclusters observed over time is of the form …”
Section: Methodsmentioning
confidence: 99%
“…Eq. 2 was derived from the results presented in ( Nino and Milstein, 2021 ) by assuming k on ≪ k off with no restriction on k bleach and by accounting for p miss . Similarly, the cumulative number of preclusters observed over time is of the form …”
Section: Methodsmentioning
confidence: 99%