Estimating Total Nephron Number in the Adult Kidney Using the Physical Disector/Fractionator Combination

Cullen‐McEwen, Luise A.; Douglas-Denton, Rebecca Nicole; Bertram, John F.

doi:10.1007/978-1-61779-851-1_30

Cited by 46 publications

(39 citation statements)

References 44 publications

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“…To calculate Nglom TOTAL , as well as the numbers of Nglom NSG and Nglom GSG , we first estimated Nglom TOTAL using the physical disector/fractionator combination at Monash University. Details of this method have been published previously (48,49), but are briefly described here. Kidneys were perfusion-fixed with 10% formalin, and a known weight fraction of tissue was sampled (fraction 1).…”

Section: Methodsmentioning

confidence: 99%

New insights on glomerular hyperfiltration: a Japanese autopsy study

Kanzaki¹,

Puelles²,

Cullen‐McEwen³

et al. 2017

JCI Insight

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Section: Methodsmentioning

confidence: 99%

New insights on glomerular hyperfiltration: a Japanese autopsy study

Kanzaki¹,

Puelles²,

Cullen‐McEwen³

et al. 2017

JCI Insight

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“…Estimation of Total Glomerular Numbers per Kidney-N(glom,kid) was estimated using another half of kidney by the physical dissector/fractionator combination method described in detail elsewhere (47).…”

Section: Stereological Analysis Of Podocyte Number and Process Lengthmentioning

confidence: 99%

Glycogen Synthase Kinase 3β Orchestrates Microtubule Remodeling in Compensatory Glomerular Adaptation to Podocyte Depletion

Liu

et al. 2015

Journal of Biological Chemistry

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Background: Microtubule dynamics is involved in podocyte morphogenesis. Results: The microtubule-associated proteins Tau and CRMP2 are regulated by GSK3␤ in podocytes. Blockade of GSK3␤ ameliorates podocyte injury and proteinuria. Conclusion: The GSK3␤-dictated microtubule remodeling is crucial for the compensatory glomerular adaptation to podocyte loss. Significance: Inhibition of GSK3␤ might represent a novel therapeutic strategy to treat podocytopathy.

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“…N glom and V glom were estimated using the physical dissector/fractionator combination as previously described. 19,28,29 In brief, perfusion-fixed kidneys were sampled to provide an isotropic, uniform random sample of tissue blocks. These blocks were embedded in glycolmethacrylate and then exhaustively sectioned at 20 mm.…”

Section: Stereologymentioning

confidence: 99%

APOL1 Risk Alleles Are Associated with Exaggerated Age-Related Changes in Glomerular Number and Volume in African-American Adults

Hoy

Hughson

Kopp

et al. 2015

Journal of the American Society of Nephrology

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APOL1 genetic variants contribute to kidney disease in African Americans. We assessed correlations between APOL1 profiles and renal histological features in subjects without renal disease. Glomerular number (N glom ) and mean glomerular volume (V glom ) were measured by the dissector/fractionator method in kidneys of African-American and non-African-American adults without renal disease, undergoing autopsies in Jackson, Mississippi. APOL1 risk alleles were genotyped and the kidney findings were evaluated in the context of those profiles. The proportions of African Americans with none, one, and two APOL1 risk alleles were 38%, 43%, and 19%, respectively; 38% of African Americans had G1 allele variants and 31% of African Americans had G2 allele variants. Only APOL1-positive African Americans had significant reductions in N glom and increases in V glom with increasing age. Regression analysis predicted an annual average loss of 8834 (P=0.03, sex adjusted) glomeruli per single kidney over the first 38 years of adult life in African Americans with two risk alleles. Body mass index above the group medians, but below the obesity definition of $30 kg/m 2 , enhanced the expression of age-related changes in N glom in African Americans with either one or two APOL1 risk alleles. These findings indicate that APOL1 risk alleles are associated with exaggerated age-related nephron loss, probably decaying from a larger pool of smaller glomeruli in early adult life, along with enlargement of the remaining glomeruli. These phenomena might mark mechanisms of accentuated susceptibility to kidney disease in APOL1-positive African Americans.

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Estimating Total Nephron Number in the Adult Kidney Using the Physical Disector/Fractionator Combination

Cited by 46 publications

References 44 publications

New insights on glomerular hyperfiltration: a Japanese autopsy study

New insights on glomerular hyperfiltration: a Japanese autopsy study

Glycogen Synthase Kinase 3β Orchestrates Microtubule Remodeling in Compensatory Glomerular Adaptation to Podocyte Depletion

APOL1 Risk Alleles Are Associated with Exaggerated Age-Related Changes in Glomerular Number and Volume in African-American Adults

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