Estimation of reference interval for neutrophil activity evaluation systems: a interim report

Abstract

Neutrophils play an important role in innate immunity and produce reactive oxygen species (ROS), but they can also cause inflammation and oxidative stress that can damage their own tissues. We have developed neutrophil activity evaluation systems that simultaneously monitors superoxide radicals (O2 •-) and hypochlorite ions (OCl -) secreted by stimulated neutrophils in a few microliters of whole blood and have conducted clinical studies in humans. Here, we report normal reference intervals with our systems based on the results of 3082 persons who underwent comprehensive cancer screening between February 2020 and March 2022. A total of 344 were extracted as reference individuals based on the results of the cancer screening and the reference intervals of the two systems were interim estimated considering gender and age. Reference intervals can be used as a marker of sub-clinical inflammation, which is difficult to detect with other blood markers.