Estimation of the oxidative stress in moss Pohlia nutans (Hedw.) Lindb. depending on the influence of gravity

Kyyak, N.Ya.; Khorkavtsiv, Ya.D.

doi:10.15407/knit2016.04.058

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…They absorb nutritious substances from precipitation and atmospheric air, and keep them in the undecomposed part of the moss turfs (Turetsky et al, 2012;Kyyak & Baik, 2016). This can be explained by the growing conditions of mosses (high level of the humidity, high actual acidity, low temperature) and physical and chemical peculiarities of the moss cells (high cation exchange capacity and high concentrations of phenolic compounds and lipids in the cell walls) (Lobachevska et al, 2005;Seedre & Chen, 2010;Lobachevska, 2014;Kyyak & Khorkavtsiv, 2016). In addition to the storage of mineral nutrients, bryophytes are able to accumulate carbon.…”

Section: Discussionmentioning

confidence: 99%

Role of the bryophytes in substrate revitalization on a post-technogenic salinized territory

et al. 2020

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This work aims to investigate the role of the bryophyte cover in substrate revitalization on a post-technogenic salinized territory. The influence of the moss cover on the organic carbon content, actual acidity, redox potential, and the content of the main ecological and trophic groups of microorganisms in the substrate of the tailings storage of the Stebnyk Mining and Chemical Enterprise "Polymineral" was investigated. Bryophytes significantly affect the tailings storage saline substrates. They colonize areas with a very strong and strong degree of salinity, which are unsuitable for other plants. It was indicated that pioneer moss species promote the accumulation of organic matter in saline substrates of the tailings storage. Under moss turfs, the amount of organic carbon increased 2.2–5.0 times, compared with its content in the uncovered substrate. The high variability of the organic matter content is determined by the species characteristics of mosses, primarily their life form. The dense-turf species Didymodon rigidulus and Pthychostomum pseudotriquetrum var. bimum accumulated the most organic matter. The thickness of the litter under the moss turf of these species was much greater than in Barbula unguiculata and Funaria hygrometrica, which form loose turf. We assessed the specificity of the accumulation of organic carbon in the turfs of the studied mosses. It founded that most organic matter accumulated in the dead parts of the moss turf. In the green parts of the shoots of these moss species the amount of organic carbon was 3–4 times less, which indicates a relationship between litter capacity and content of carbon in the substrate under moss turfs. We investigated the influence of mosses on the actual acidity of the tailings storage substrate. Moss turfs promote the increase of acidity of the aqueous solution of the tailings substrate by 0.2–0.5 units. The tailings storage substrates are characterized by a reduction regime. The redox potential of the substrate under moss cover significantly depended on the species characteristics of mosses. Under the moss cover, the redox potential increased by 1.2–1.4 times, compared with the index for the substrate without moss cover. We studied the influence of moss cover on microbial biomass and the quantity of some ecological-trophic groups of microorganisms in the substrates of the tailings storage. The amount of microbial biomass under moss turfs increased depending on the degree of the substrate salinization and the species characteristics of the mosses. In areas with a very high degree of salinization under the moss turfs of Didymodon rigidulus and Funaria hygrometrica, the microbial biomass index increased almost two times, compared with the uncovered substrate. We found a significant increase in the quantity of the main ecological and trophic groups of microorganisms (saprophytes, cellulose-destroying bacteria, oligonitrophils and nitrogen fixers) in the substrate under the moss cover. Thus, pioneer moss species have a complex effect on the saline substrate of tailings storage. They accumulate organic matter, increase the acidity of the upper layer, improve the redox regime of the substrate and promote the development of soil microbiota.

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Section: Discussionmentioning

confidence: 99%

Role of the bryophytes in substrate revitalization on a post-technogenic salinized territory

et al. 2020

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“…Carbohydrates also intercept reactive oxygen species and inhibit the processes of free radical oxidation of biological molecules during the development of oxidative stress induced by salinity. In addition to its protective and antioxidant effects, some carbohydrates can play a signaling role (Flowers et al, 2014;Kyyak & Khorkavtsiv, 2016;Lobachevska et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Ecological and physiological peculiarities of bryophytes on a post-technogenic salinized territory

2019

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Taxonomic, biomorphological and ecological structures of bryophytes, their reproductive strategy and the main mechanisms of tolerance in the conditions of salinization were investigated. Bryophytes are the pioneers that have colonized the territory of a tailing storage that holds liquid waste from potassium-magnesium concentrate production of the Mining and Chemical Enterprise "Polymineral". Due to excess salts, the soil solution in the shore area of the tailing pond acquires high osmotic pressure. Three experimental plots which differed significantly in the level of the substrate salinity were laid at the distance of 3, 6 and 9 m from the reservoir for experimental studies. Water extracts of the substrates from the test sites showed the highest concentrations for sulfates – 10.4–64.6 mg Eq/100 g of soil and chlorides – 7.6–43.3 mg Eq/100 g of soil. It was established that the investigated areas of the tailing storage territory differed in the biochemical activity of the substrate, which was evaluated by its redox potential. On the areas of the uncovered substrate it was the lowest – 230 mV, which indicates anaerobiosis in conditions of very high salinization and moisture. Higher ROP values were determined at the sites of bryophyte cover distribution – 295–330 mV. The aim of the study was to determine the features of taxonomic, biomorphological and ecological structures of bryophytes, their reproductive strategy and to establish the main mechanisms of adaptation to the conditions of salinization on the tailing storage territory. 24 species and 3 varieties of bryophytes, belonging to 12 families and 16 genera were found on the shore of the tailing storage pond. The results of biomorphological and ecological analysis of bryophytes indicate the uneven conditions of the habitats and their considerable ecological plasticity. Among the bryophytes, mesophytes, xeromesophytes and meso-eutrophs, eutrophs with a life-form of low dense and loose turf dominated. In salinization conditions, dioicous acrocarpous mosses prevailed, the fertile turf of which, depending on the influence of abiotic factors, differed significantly in the number of sexual shoots, their ratio and productivity. Bulbils were found only on the tips of Bryum argenteum shoots. Along with Salicornia europaea L., a euhalophyte, the leading role in the initial stage of overgrowth of the tailing storage area most often belonged to Didymodon rigidulus, Bryum argenteum, Funaria hygrometrica and Barbula unguiculata. The process of formation of bryophyte cover occurred along a gradient of decrease in salt concentration at the experimental sites. Adaptation of bryophytes to substrate salinity is due to a change in metabolic processes, which is manifested in an increase of the total content of carbohydrates and an increase of the cation exchange capacity of moss cell walls, which is the primary barrier that reduces the toxic effect of ions under salt stress.

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“…У рослинах B. caespiticium з Антарктики антиоксидантна активність була в 1. 5 [14,[20][21][22]42]. Рослини з екстремальних кліматичних умов найбільш придатні для таких досліджень [15,34,38].…”

Section: морфофізіологічні реакції гравічутливості та адаптації до уф-опромінення моху Bryum Caespiticium Hedw з антарктикиunclassified

Morpho-physiological reactions of gravisensitivity and adaptation to UV-radiation of the moss Bryum Caespiticiumhedw. from Antarctica

Kyyak¹,

Lobachevska²,

Khorkavtsiv³

2021

Kosm. nauka tehnol.

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The adaptive physiological reactions of the moss Bryum caespiticium Hedw. from Antarctica to the influence of UV radiation and gravimorphoses as a factor of adaptive plasticity, associated with environmental conditions, were studied. As a control, B. caespiticium plants were collected in the Nature Reserve “Roztochchia” (Lviv region). In investigations, we used a sterile laboratory culture of mosses grown under controlled conditions in a phytotron. Moss shoots were irradiated with UV rays generated by an ultraviolet lamp OSRAM with an intensity of 4 kW/m2, which caused 50 % inhibition of plant regeneration (ED50). Physiological parameters were determined 24 h after exposure to UV radiation. The influence of gravity on the morphological form of B. caespiticium gametophyte turf and the interaction of light and gravity in gravi-/phototropism as a manifestation of gravimorphoses adaptability were analyzed. One of the objectives was to investigate the formation of gravimorphoses as a result of the initiation of cells’ branching processes and the formation of gametophore buds and to evaluate their role in the life cycle of B. caespiticium under extreme conditions. For this, we determined the branching coefficient of the gravitropic protonema, the inclination angle of the branches and the buds’ development depending on the interaction of photo- and gravitropism, under the influence of red and blue light, and the effect of UV on gravisensitivity. The influence of physiologically active red and blue light on the branching activity and bud formation on the gravitropic protonema of the Antarctic moss B. caespiticium was investigated. It was found that red light mainly inhibited graviperception and gravitropic growth of protonemata cells, resulting in a change of the response to gravity, but initiated high branching activity and, accordingly, another morphological form of turf. After the influence of the blue light, intensive bud formation and gametophore development were observed. Thus, gravitation promoted morphological variability and changes in the functional activity of cells at the juvenile stage of the protonemata development, which is important for the survival of the moss under extreme environmental conditions. After UV irradiation the gravisensitivity of the B. caespiticium protonemata decreased. However, due to the resistance of the moss sample from Antarctica to the prolonged influence of UV rays, gravitropic growth was not completely blocked, as in plants from the Lviv region. The effect of the ultraviolet irradiation on the antioxidant activity, the content of soluble (vacuolar) and cell wall-bound fractions of UV-absorbing phenolic components, flavonoids content and their absorption spectra, as well as the amount of carotenoids and anthocyanins in B. caespiticium shoots, were determined. It was established that B. caespiticium plants from Antarctica have 1.5 times higher antioxidant activity compared to plants from the Lviv region, which confirms the high level of protection against oxidative damage. UV irradiation activates the synthesis of UV-absorbing phenolic compounds in mosses. The shoots of B. caespiticium from Antarctica defined a higher content of phenols compared to samples from the Lviv region and their significant increase under the influence of UV radiation. The content of UV-absorbing compounds bound with the cell wall was higher than the concentration of soluble phenolic compounds, both in plants from Antarctica and in samples from the Lviv region, which indicates their participation in the mechanisms of cells protection from UV radiation. It was shown that the influence of UV irradiation induced an increase of flavonoids’ content in the shoots of both samples of B. caespiticium, but for plants from Antarctica, the concentration of flavonoids after stress was 1.7 times higher than in plants from the Lviv region. The absorption spectra of flavonoids revealed flavonols rutin and quercetin and flavone luteolin in both samples of B. caespiticium, which provide effective cells absorption of UV rays. The higher content of anthocyanins and carotenoids in moss shoots from Antarctica both in the control sample and after the exposure to UV radiation promotes the protection against damage and formation of the adaptive potential.

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Estimation of the oxidative stress in moss Pohlia nutans (Hedw.) Lindb. depending on the influence of gravity

Cited by 7 publications

References 11 publications

Role of the bryophytes in substrate revitalization on a post-technogenic salinized territory

Role of the bryophytes in substrate revitalization on a post-technogenic salinized territory

Ecological and physiological peculiarities of bryophytes on a post-technogenic salinized territory

Morpho-physiological reactions of gravisensitivity and adaptation to UV-radiation of the moss Bryum Caespiticiumhedw. from Antarctica

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