Estimation of total bacteria by real-time PCR in patients with periodontal disease

Brajović, Gavrilo; Popović, Branka; Puletic, Miljan; Kostić, Marija; Milašin, Jelena

doi:10.2298/sarh1602010b

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…Microorganisms settled in the oral environment may have been detected by chance. Therefore, to be able to predict more accurately the role that herpesviruses have in the development and severity of periodontitis, performing quantitative PCR analyses 17 could be advantageous.…”

Section: Discussionmentioning

confidence: 99%

“…The subgingival samples were collected from the periodontitis active sites exhibiting the deepest pocket depths using three paper points that were inserted in the periodontal pockets for 60 s. The paper points were subsequently transferred into 1 mL of reduced transport fluid (RTF) and stored at −70°C, according to a modified protocol for sample collection. 17,18 The extraction of bacterial DNA from the collected samples was done using a Qiamp DNA Mini Kit (Qiagen, Hilden, Germany). Viral DNA was obtained by incubating 500 μL of samples with 10 μL of proteinase K at 55°C, and then proteinase K was inactivated by boiling at 100°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

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Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease

Puletic

Popović

Janković

et al. 2020

Microbiology and Immunology

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The aim was to investigate the detection rates of periodontal bacteria (Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans) and herpesviruses (herpes simplex virus-1 [HSV-1], cytomegalovirus [CMV], and Epstein-Barr virus [EBV]) in different forms and severity of periodontal disease, and to compare them with those in periodontally healthy subjects. One hundred and twenty-nine patients participated in the study: 39 diagnosed with periodontal abscess (PA), 33 with necrotizing ulcerative periodontitis (NUP), 27 with chronic periodontitis (CP), and 30 participants with healthy periodontal tissue represented a healthy control group. All patients with periodontal disease (PA, NUP, and CP) were also divided into two groups according to the severity of their disease: moderate and severe periodontitis. The subgingival samples were collected from the periodontitis active sites and the detection of microorganisms was performed by end-point polymerase chain reaction analyses. The results revealed significantly higher detection rates of P. gingivalis, T. forsythia, and P. intermedia in all three groups of patients with periodontitis than in healthy participants. The highest detection rate of A. actinomycetemcomitans was noticed in CP, which was significantly higher than that in PA, NUP, and healthy control. The occurrence of EBV was significantly higher in NUP than in CP and healthy participants. CMV was detected significantly more frequently in PA and NUP than in CP and healthy participants. Comparisons among healthy participants and patients with moderate and severe periodontitis showed significantly higher detection rates of EBV and

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease

Puletic

Popović

Janković

et al. 2020

Microbiology and Immunology

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“…The principal goal of the periodontal therapy is to restrain active inflammation during the disease and possibly provide support for the reconstruction of periodontal tissue defects [5]. Initial periodontal therapy, scaling and root planning (SRP) is not frequently resolute at repairing disease-related defects [6,7]. The periodontal wound healing after SRP usually induces the development of a long junctional epithelium, which is responsible for frequent recurrence of a periodontal pocket [8].…”

Section: Introductionmentioning

confidence: 99%

The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis

et al. 2020

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Introduction/Objective The objective of the study was to investigate whether there are differences in therapeutic effect between initial treatments of chronic periodontitis [scaling and root planning (SRP)] alone and SRP in conjunction with injectable platelet-rich fibrin (I-PRF) application, comparing clinical parameters after three months. Methods Twenty-four patients with chronic periodontitis who had at least two sites with probing pocket depth (PPD) ≥ 5 mm on contralateral side participated in the study. Using a split-mouth design, the patients were treated with SRP + I-PRF (study group) or SRP only (control group). The clinical parameters, clinical attachment level (CAL), gingival margin level (GML), PPD, bleeding on probing, and plaque index, were recorded on both sides. Results Compared to baseline, both treatment modalities demonstrated an improvement in investigated clinical parameters. The mean value of CAL was reduced from 1.97 ± 0.75 (0.25-3.31) to 1.07 ± 0.44 (0.12-1.78) in the study group, whereas it decreased from 1.81 ± 0.66 (0.42-2.96) to 1.48 ± 0.55 (0.22-2.30) in the control group. Similarly, the corresponding values for GML and PPD showed statistically significant difference between the groups (p = 0.040 and p = 0.006, respectively). Conclusion Regardless the limited number of patients in the study, initial periodontal therapy in conjunction with injectable platelet-rich fibrin proved to display significant improvement in all clinical parameters compared to initial periodontal therapy alone.

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“…DNA extracts were stored at -20ºC prior to PCR analysis. Total gene copy number determination was done as described by Brajović et al [16], using Maxima™ SYBR Green/ROX qPCR Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) and the following primers: Fw 5'-TCCTACGGGAGCACAGT'-3 and Rv (5'GGACTACCAGGGTATCTAATCCTGTT-3' . Real-time PCR analyses were performed on Line Gene-K Fluorescence Real-time PCR Detection System (Hangzhou Bioer Technology Co. Ltd., Hangzhou, China).…”

Section: Microorganisms' Quantificationmentioning

confidence: 99%

Microbial adherence affinity and clinical characteristics of polypropylene versus silk sutures in oral surgery

et al. 2018

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Introduction/Objective The purpose of this study was to compare polypropylene and silk suture materials in terms of bacterial adherence and clinical features including the impact on soft tissue healing. Methods Ten healthy patients were included in this study. Unilateral upper and lower wisdom teeth were extracted at the same time and wounds were sutured with different threads (one monofilamentpolypropylene-and one multifilament-silk suture). Stitches were removed seven days postoperatively. Real-time polymerase chain reaction was used to analyze bacterial adherence. Intraoperative handling and ease of removal were assessed with the help of Visual Analogue Scale. Landry healing index was used for evaluation of soft tissue healing. Results Significantly more pronounced bacterial adherence was found on silk compared to polypropylene sutures (p = 0.005). Superior intraoperative handling properties were registered suturing with polypropylene compared to silk (p = 0.005). Soft tissue healing was significantly better around polypropylene sutures, both on the third and the seventh postoperative day (p = 0.016). Patient discomfort was slightly higher for polypropylene sutures, but without statistical significance. Conclusion Polypropylene suture material showed significantly lower bacterial adherence and superior clinical features compared to silk, including better soft tissue healing.

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Estimation of total bacteria by real-time PCR in patients with periodontal disease

Abstract: The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis.

Cited by 6 publications

References 28 publications

Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease

Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease

The effect of injectable platelet-rich fibrin use in the initial treatment of chronic periodontitis

Microbial adherence affinity and clinical characteristics of polypropylene versus silk sutures in oral surgery

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