Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro

Tasaki, Hidetaka; Iwata, Hisataka; Sato, Daichi; Monji, Yasunori; Kuwayama, Takehito

doi:10.1016/j.theriogenology.2012.12.009

Cited by 35 publications

(27 citation statements)

References 16 publications

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“…The number of genes with significantly enhanced or reduced expression was counted for each period of follicle development (EAFs to SAFs, SAFs to LAFs, and SAFs to IVGs). In our preliminary experiments and previous reports involving the same culture methods [1, 10, 11], oocyte diameter (average 112 μm) and the ability to complete nuclear maturation (42%) were found to be similar to those in oocytes of SAFs. Accordingly, here, we compared gene expression between IVGs and SAFs.…”

Section: Resultssupporting

confidence: 78%

Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in <i>in vitro</i> grown oocyte-and-granulosa cell complexes

Munakata

Kawahara-Miki

Shiratsuki

et al. 2016

Journal of Reproduction and Development

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Follicle development is accompanied by proliferation of granulosa cells and increasing oocyte size. To obtain high-quality oocytes in vitro, it is important to understand the processes that occur in oocytes and granulosa cells during follicle development and the differences between in vivo and in vitro follicle development. In the present study, oocytes and granulosa cells were collected from early antral follicles (EAFs, 0.5–0.7 mm in diameter), small antral follicles (SAFs, 1–3 mm in diameter), large antral follicles (LAFs, 3–7 mm in diameter), and in vitro grown oocyte-and-granulosa cell complexes (OGCs), which were cultured for 14 days after collection from EAFs. Gene expression was analyzed comprehensively using the next-generation sequencing technology. We found top upstream regulators during the in vivo follicle development and compared them with those in in vitro developed OGCs. The comparison revealed that HIF1 is among the top regulators during both in vivo and in vitro development of OGCs. In addition, we found that HIF1-mediated upregulation of glycolysis in granulosa cells is important for the growth of OGCs, but the cellular metabolism differs between in vitro and in vivo grown OGCs. Furthermore, on the basis of comparison of upstream regulators between in vivo and in vitro development of OGCs, we believe that low expression levels of FLT1 (VEGFA receptor), SPP1, and PCSK6 can be considered causal factors of the suboptimal development under in vitro culture conditions.

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Section: Resultssupporting

confidence: 78%

Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in <i>in vitro</i> grown oocyte-and-granulosa cell complexes

Munakata

Kawahara-Miki

Shiratsuki

et al. 2016

Journal of Reproduction and Development

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“…Thus, although we could not identify a direct cause for the high responsiveness of OGCs derived from aged cows to E 2 , our results suggest that post-transcriptional modification of estradiol receptors differs between the two age groups. The responsiveness to E 2 differs among different developmental stages of follicle, and OGCs derived from advanced stage EAFs did not require much E 2 for their in vitro development [30]. In addition, we found that OGCs derived from EAFs of aged cows were at a more advanced stage compared to those of the younger counterparts.…”

Section: Discussionmentioning

confidence: 63%

“…These media were IVC medium containing 0.1 μg/mL estradiol and an IVC medium containing 10 μg/mL estradiol. Concentration of estradiol was set on the basis of the previous reports on estradiol concentration of bovine follicles [23], and that high concentration of estradiol 10 μg/mL enhanced in vitro growth of oocytes of EAFs derived from young cows and gilts [24,30]. For replicates, about 30-40 oocytes were collected from the group of young or aged cows (Table 1) and the experiment was repeated 10 times.…”

Section: Methodsmentioning

confidence: 99%

Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles

Itami

Kawahara-Miki

Kawana

et al. 2014

J Assist Reprod Genet

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“…As estradiol reduces IGFBP3 (Huynh, Yang, & Pollak, ), use of estradiol in the medium could be reasonably expected to rescue the cultured follicles from the adverse effects. Indeed, estradiol was reported to improve survival, diameter and antrum formation in the cultured PFs in the pig (Tasaki, Iwata, Sato, Monji, & Kuwayama, ), cow (Hulshof et al., ), rat (Gore‐Langton & Daniel, ) and sheep (Kona et al., personal communication). Thus there appears to be a strong reason to counteract the inimical effects of IGFBP3 by improving the oestrogen secretion or addition of oestrogen to the cultured medium.…”

Section: Discussionmentioning

confidence: 99%

Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

Srividya

Chakravarthi

Kona

et al. 2017

Reprod Domestic Animals

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Expression of Kit ligand (KL) and insulin-like growth factor binding protein (IGFBP3) genes was studied at different in vivo and corresponding in vitro stages of development of the ovarian follicles in sheep. The expression of both KL and IGFBP3 was significantly higher in the primordial follicles relative to any other stage of development. Compared to the other stages, the KL expression in the cumulus cells from in vivo grown large antral follicles and that of IGFBP3 in COCs' isolated from large antral follicles matured in vitro for 24 hr were significantly higher. In the oocytes from in vivo grown ovarian follicles, the expression of KL was the same at all the stages of development. Insulin-like growth factor binding protein 3 expression was also the same in the oocytes at all the stages of the development except for a significantly lower expression in those from antral follicles. The expression of KL in the cumulus cells decreased significantly in the in vitro grown early antral follicles but did not change further as the development progressed. The expression of IGFBP3 in the cumulus cells from in vitro grown ovarian follicles appeared to increase as the development progressed although the increase was not significant between any two consecutive stages of development. In the oocytes in in vitro grown ovarian follicles, the expression levels of KL and IGFBP3 genes did not change with development. It is concluded that (i) KL and IGFBP3 genes follow specific patterns of expression during ovarian folliculogenesis and (ii) in vitro culture of preantral follicles compromises the development potential through alterations in the stage-specific patterns of expression of these and other developmentally important genes.

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Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro

Cited by 35 publications

References 16 publications

Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in <i>in vitro</i> grown oocyte-and-granulosa cell complexes

Gene expression patterns in granulosa cells and oocytes at various stages of follicle development as well as in <i>in vitro</i> grown oocyte-and-granulosa cell complexes

Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles

Expression of kit ligand and insulin‐like growth factor binding protein 3 during in vivo or in vitro development of ovarian follicles in sheep

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