Estrogen enhances the bone regeneration potential of periodontal ligament stem cells derived from osteoporotic rats and seeded on nano-hydroxyapatite/collagen/poly(L-lactide)

Lingling, E; Xu, Wenqing; Feng, Lin; Liu, Yi; Cai, Dongqing; Wen, Ning; Zheng, Wenjie

doi:10.3892/ijmm.2016.2559

Cited by 36 publications

(17 citation statements)

References 53 publications

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“…Beyond its role as a sex hormone, estrogen has important roles in the development, maturation, and aging of extragonadal tissues such as bone (Hansen et al, 2009a; Cui et al, 2013; Ling-Ling et al, 2016), muscle (Dieli-Conwright et al, 2009; Enns and Tiidus, 2010), and connective tissues (Hansen et al, 2009a,b; Hansen and Kjaer, 2014; Hansen, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effect of Estrogen on Musculoskeletal Performance and Injury Risk

2019

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Estrogen has a dramatic effect on musculoskeletal function. Beyond the known relationship between estrogen and bone, it directly affects the structure and function of other musculoskeletal tissues such as muscle, tendon, and ligament. In these other musculoskeletal tissues, estrogen improves muscle mass and strength, and increases the collagen content of connective tissues. However, unlike bone and muscle where estrogen improves function, in tendons and ligaments estrogen decreases stiffness, and this directly affects performance and injury rates. High estrogen levels can decrease power and performance and make women more prone for catastrophic ligament injury. The goal of the current work is to review the research that forms the basis of our understanding how estrogen affects muscle, tendon, and ligament and how hormonal manipulation can be used to optimize performance and promote female participation in an active lifestyle at any age.

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Section: Introductionmentioning

confidence: 99%

Effect of Estrogen on Musculoskeletal Performance and Injury Risk

2019

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“…This dynamic process is closely modulated by a variety of hormones and endocrine signals. Among these, estrogen plays a significant role and exerts its action through two nuclear receptors, namely estrogen receptor α (ERα) and β (ERβ), in skeletal growth for both genders 3.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Estradiol on the Growth Plate Chondrocytes of Limb and Spine from Postnatal Mice in vitro: The Role of Estrogen-Receptor and Estradiol Concentration

Shi

Zheng

et al. 2017

Int. J. Biol. Sci.

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Objectives: Skeletal development is a complex process. Little is known about the different response of limb or spine growth plate chondrocytes (LGP or SGP) to the estrogen level and the role of estrogen receptor (ER) during postnatal stage.Methods: LGP and SGP chondrocytes were isolated from 50 one-week mice and treated with different concentrations of 17β-estradiol. Cell viability was measured by cell counting kit-8 (CCK-8). The expression of collagen II and X were evaluated by real-time PCR and Western blotting. Then, the response of LGP or SGP chondrocyte after with or without estradiol and specific ER antagonists to block the effect of ERs were also measured by Western blotting and immunofluorescence.Results: Estradiol promoted the chondrogensis of the chondrocytes in vitro and achieved the maximal expression of type II collagen at the dose of 10-7 M. Additionally, the regulatory effect of estradiol on the chondrogenesis can be mainly relied on ERα. The LGP chondrocytes were more sensitive to the estradiol treatment than SGP in the expression of type II collagen.Conclusions: Estrogen at a pharmacological concentration (10-7 M) could stimulate the maximal production of type II collagen in the growth plate chondrocytes in vitro, which exerts its activity mainly through ERα in the chondrogenesis. Furthermore, the LGP chondrocytes were more sensitive to the estradiol treatment than SGP in the chondrogenesis.

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“…BMSCs are one of the most commonly used cells in basic research and the clinical treatment of orthopedic diseases (18); BMSCs provide not only cell sources, but also cytokines and growth factors to accelerate the healing process of femoral head necrosis (19). PLA, hydroxyapatite (HA) and collagen are widely used in the manufacture of bionic artificial bone materials, which have achieved good results in clinical treatment (19).…”

Section: Discussionmentioning

confidence: 99%

The effect of bone marrow mesenchymal stem cell and nano‑hydroxyapatite/collagen I/poly‑L‑lactic acid scaffold implantation on the treatment of avascular necrosis of the femoral head in rabbits

Wang

Peng

et al. 2019

Exp Ther Med

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For avascular necrosis of the femoral head (ANFH), repair and regeneration are difficult because of the edema and high pressure caused by continuous ischemia and hypoxia. Core decompression (CD) is a classic method for treating early ANFH before the collapse of the femoral head; however, its effect is still controversial. To improve the therapeutic effect of CD on ANFH, a novel tissue-engineered bone (TEB) was constructed by combining bone marrow mesenchymal stem cells (BMSCs) with nano-hydroxyapatite/collagen I/poly-L-lactic acid (nHAC/PLA) scaffolds and implanting the TEB into the bone tunnel of CD. Cell attachment was observed by scanning electron microscopy and hematoxylin and eosin staining. The authors' previous studies confirmed that nHAC/PLA is an excellent scaffold material with favorable biocompatibility and no cytotoxicity. A total of 24 New Zealand rabbits with ANFH were randomly divided into three groups, as follows: Group A (n=8), pure CD; group B (n=8), CD+nHAC/PLA; and group C (n=8), CD+BMSCs-nHAC/PLA. The favorable effect of BMSCs-nHAC/PLA on angiogenesis and bone formation in necrotic areas was further evaluated via radiographic and histological analyses. Computerized tomography (CT) scanning and H&E staining showed more capillaries and new osteoid tissue in group C compared with in groups B and A. Micro-CT showed that the new bone coverage rate and implanted material degradation degree were each increased in group C compared with in group B. These results indicate that BMSCs-nHAC/PLA scaffolds may improve the curative effect of CD and provide a strategy for treating ANFH.

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Estrogen enhances the bone regeneration potential of periodontal ligament stem cells derived from osteoporotic rats and seeded on nano-hydroxyapatite/collagen/poly(L-lactide)

Cited by 36 publications

References 53 publications

Effect of Estrogen on Musculoskeletal Performance and Injury Risk

Effect of Estrogen on Musculoskeletal Performance and Injury Risk

The Effect of Estradiol on the Growth Plate Chondrocytes of Limb and Spine from Postnatal Mice in vitro: The Role of Estrogen-Receptor and Estradiol Concentration

The effect of bone marrow mesenchymal stem cell and nano‑hydroxyapatite/collagen I/poly‑L‑lactic acid scaffold implantation on the treatment of avascular necrosis of the femoral head in rabbits

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