Estrogen Promotes Angiogenic Activity in Human Umbilical Vein Endothelial Cells In Vitro and in a Murine Model

Morales, David; McGowan, Kelly A.; Grant, Derrick S.; Maheshwari, Shailendra; Bhartiya, Deepa; Cid, María C.; Kleinman, Hynda K.; Schnaper, H. William

doi:10.1161/01.cir.91.3.755

Cited by 401 publications

(237 citation statements)

References 41 publications

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“…28,29 It is known that estradiol promotes endothelial cell activities. 30,31 In our group, the duration of estrogen administration varied from 5 days to 16 weeks with a median duration of 5.5 weeks. Seven of 10 patients with HC showed positive effects with resolving haematuria.…”

Section: Discussionmentioning

confidence: 88%

Encouraging results in the treatment of haemorrhagic cystitis with estrogen – report of 10 cases and review of the literature

Ordemann

Naumann

Geissler

et al. 2000

Bone Marrow Transplant

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Summary:Haemorrhagic cystitis (HC) after allogeneic haematopoietic stem cell transplantation (HSCT) or high-dose cyclophosphamide (CP) chemotherapy is a severe sideeffect and can cause significant morbidity and mortality. In this report, we describe the clinical courses of 10 patients with HC and review the literature. The patients were treated with oral conjugated estrogen in an attempt to improve severe haemorrhagic cystitis. In seven patients positive effects were seen, haematuria resolved in all, but residual symptoms of dysuria remained for longer periods. In one patient application of estrogen was interrupted because of hepatotoxicity. Two patients failed all treatment modalities including oral estrogen because of terminal illness. We conclude that in the management of HC the administration of oral conjugated estrogen should be considered. Bone Marrow Transplantation (2000) 25, 981-985.

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Section: Discussionmentioning

confidence: 88%

Encouraging results in the treatment of haemorrhagic cystitis with estrogen – report of 10 cases and review of the literature

Ordemann

Naumann

Geissler

et al. 2000

Bone Marrow Transplant

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“…In addition, estrogen enhanced the strength of the wound at day 80 suggesting a role for the hormone in possible therapeutics to promote faster wound healing in the aged. Estrogen has also been shown to induce angiogenesis, a process necessary for proper wound healing [73]. Using an in vitro model, estrogen (10 −8 mol/L) given to a scraped monolayer of endothelial cells showed increased cell migration, cell proliferation, and organization into tubular networks [73].…”

Section: Macrophagesmentioning

confidence: 99%

“…Estrogen has also been shown to induce angiogenesis, a process necessary for proper wound healing [73]. Using an in vitro model, estrogen (10 −8 mol/L) given to a scraped monolayer of endothelial cells showed increased cell migration, cell proliferation, and organization into tubular networks [73]. These data demonstrate that estrogen promotes wound healing in a population controlled for circulating estrogen levels and suggest that the hormone is capable of exhibiting direct effects on multiple cell types involved in the healing process.…”

Section: Macrophagesmentioning

confidence: 99%

Sex differences and estrogen modulation of the cellular immune response after injury

Bird¹,

Karavitis²,

Kovacs³

2008

Cellular Immunology

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Cell-mediated immunity is extremely important for resolution of infection and for proper healing from injury. However, the cellular immune response is dysregulated following injuries such as burn and hemorrhage. Sex hormones are known to regulate immunity, and a well-documented dichotomy exists in the immune response to injury between the sexes. This disparity is caused by differences in immune cell activation, infiltration, and cytokine production during and after injury. Estrogen and testosterone can positively or negatively regulate the cellular immune response either by aiding in resolution or by compounding the morbidity and mortality. It is apparent that the hormonal dysregulation is dependent not only on the type of injury sustained but also the amount of circulating hormones. Therefore, it may be possible to design sex-specific therapies to improve immunological function and patient outcome.

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“…Estradiol, one of the components of the EaM conjugate, is known to promote proliferation and migration of human umbilical vein cells in vitro (Morales et al, 1995). Estradiol has also been shown to up-regulate VEGF mRNA in normal endometrium (Cullinan Bove and Koos, 1993) as well as in a chemically induced rat mammary carcinoma (Nakamura et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Effects of radiotherapy and estramustine on the microvasculature in malignant glioma

et al. 1999

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Summary Tumour angiogenesis is essential for progression of solid tumours and constitutes an interesting target for therapy. However, impaired tumour blood supply may also be an important obstacle for treatment by radiotherapy and chemotherapy. Estramustine has been shown to increase tumour blood flow and potentiate the effect of radiotherapy in experimental glioma. This study investigated the effects of fractionated radiotherapy and estramustine on angiogenesis in malignant glioma. The intracerebral BT4C rat glioma model was used and the animals were given whole brain radiotherapy 4 Gy × 5 days alone or in combination with estramustine 20 mg kg -1 i.p. daily. Tumour microvascular density (MVD) was assessed by manual and computerized morphometrical analysis. Expression of vascular endothelial growth factor (VEGF) was studied by in situ hybridization. Radiotherapy decreased MVD to 157 vessels per mm 2 compared to 217 vessels per mm 2 in controls. Estramustine counteracted this anti-angiogenic effect and potentiated the anti-tumoural effect of radiotherapy. In addition, vessel size increased after estramustine treatment. Five days after completion of radiotherapy the expression of VEGF was increased in the centre of the tumours. In conclusion, fractionated radiotherapy decreases microvascular density in experimental malignant glioma. This effect was abolished by estramustine. The anti-vascular effect of irradiation is important to recognize when combining radiotherapy with cytotoxic drugs.

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Estrogen Promotes Angiogenic Activity in Human Umbilical Vein Endothelial Cells In Vitro and in a Murine Model

Abstract: These studies demonstrate that, in vitro and in vivo, estradiol enhances endothelial cell activities important in neovascularization and suggest a promoting influence of estrogens on angiogenesis.

Cited by 401 publications

References 41 publications

Encouraging results in the treatment of haemorrhagic cystitis with estrogen – report of 10 cases and review of the literature

Encouraging results in the treatment of haemorrhagic cystitis with estrogen – report of 10 cases and review of the literature

Sex differences and estrogen modulation of the cellular immune response after injury

Effects of radiotherapy and estramustine on the microvasculature in malignant glioma

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