Estrogen receptor (ER) and its messenger ribonucleic acid expression in the genital tract of female mice exposed neonatally to tamoxifen and diethylstilbestrol

Satō, Tomomi; Ohta, Yasuhiko; Okamura, Hiroaki; Hayashi, Shinji; Iguchi, Taisen

doi:10.1002/(sici)1097-0185(199603)244:3<374::aid-ar9>3.0.co;2-y

Cited by 47 publications

(19 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We could confirm that Tamoxifen induced up-regulation of ERa which was obtained by previous studies on the reproductive system: Tam treatment was associated with up-regulation of ERa in seminal vesicles of neonatal male rats [10] and in the uterus and vagina of neonatal and ovariectomized adult mice [11]. These results suggest that Tamoxifen up-regulates ERa expression by increasing promoter activity of the ERa gene (Ers1) at the transcriptional level [12].…”

Section: Discussionsupporting

confidence: 88%

Tamoxifen affects glucose and lipid metabolism parameters, causes browning of subcutaneous adipose tissue and transient body composition changes in C57BL/6NTac mice

Hesselbarth

Pettinelli

Gericke

et al. 2015

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 88%

Tamoxifen affects glucose and lipid metabolism parameters, causes browning of subcutaneous adipose tissue and transient body composition changes in C57BL/6NTac mice

Hesselbarth

Pettinelli

Gericke

et al. 2015

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

“…For example, high levels of ER mRNA expression in luminal and, particularly, glandular epithelial cells preceding and during gland genesis correspond to the time during which gland genesis can be inhibited by estrogen treatment [4,13,14]. Exogenous estrogens and antiestrogens not only exert their effects by interacting with the ER protein already present in a developing tissue, they also have been shown to alter ER mRNA levels [7,[31][32][33][34].…”

Section: Discussionmentioning

confidence: 99%

Ontogeny of Estrogen Receptor Messenger Ribonucleic Acid Expression in the Postnatal Rat Uterus1

Fishman

Branham²,

Streck³

et al. 1996

Biology of Reproduction

View full text Add to dashboard Cite

During the first 2 wk of postnatal life, the rodent uterus undergoes a period of marked growth and differentiation. To further examine the role of the estrogen receptor (ER) in the mediation of uterine development, we analyzed the ontogeny of ER mRNA expression in the postnatal rat uterus using in situ hybridization. ER mRNA was present in the uterine stroma on the day of birth and progressively increased in abundance during the first 2 wk of postnatal life. In contrast, ER mRNA was not detectable in the luminal epithelium at birth and did not become abundant in this region until postnatal day (P) 7. ER mRNA abundance increased in the luminal epithelium and in the invaginating and fully formed glandular epithelium during the second week of life. At P21 ER mRNA was more abundant in the glandular epithelium than in any other uterine cell type. These results are consistent with, and extend the findings of, previous studies using uterine homogenate binding assays and immunohistochemistry to define ER ontogeny in rodents. Delineation of the temporal and cell-type specific pattern of ER mRNA ontogeny in the postnatal rat uterus furthers our understanding of the molecular basis of both endogenous and exogenous estrogen effects on uterine growth and development.

show abstract

“…On the contrary, ER␣ expression in the vagina of ␤ERKO mice at day 2 was lower than that of WT mice. ER␤ transcript variant 1, acts as a dominant negative form of ER␣ (48), is expressed both in the neonatal uterine and vaginal epithelia, however, ER␣ is expressed in the neonatal vaginal epithelium, but not in the neonatal uterine epithelium (49). Since stimulated ER␣ signaling by E2 induced down-regulation of ER␣ mRNA expression (Figure 4), ER␤ in the vaginal epithelium of neonatal mice may indirectly inhibit cell proliferation through the inactivation of ER␣.…”

Section: Discussionmentioning

confidence: 99%

Neonatal Estrogen Receptor β Is Important in the Permanent Inhibition of Epithelial Cell Proliferation in the Mouse Uterus

Nakajima

Tanimoto

Tanaka³

et al. 2015

Endocrinology

Self Cite

View full text Add to dashboard Cite

Estrogen receptor α (ERα) plays a pivotal role in the mouse uterine and vaginal epithelial cell proliferation stimulated by estrogen, whereas ERβ inhibits cell proliferation. ERβ mRNA is expressed in neonatal uteri and vaginae; however, its functions in neonatal tissues have not been ascertained. In this study, we investigated the ontogenic mRNA expression and localization of ERβ, and its roles in cell proliferation in neonatal uteri and vaginae of ERβ knockout (βERKO) mice. ERβ mRNA and protein were abundant in the uterine and vaginal epithelia of 2-day-old mice and decreased with age. In uterine and vaginal epithelia of 2-day-old βERKO mice, cell proliferation was greater than that in wild-type animals and in uterine epithelia of 90- and 365-day-old βERKO mice. In addition, p27 protein, known as a cyclin-dependent kinase inhibitor, was decreased in the uteri of 90- and 365-day-old βERKO mice. Inhibition of neonatal ERs by ICI 182780 (an ER antagonist) treatment stimulated cell proliferation and decreased p27 protein in the uterine luminal epithelium of 90-day-old mice but not in the vaginal epithelium. These results suggest that neonatal ERβ is important in the persistent inhibition of epithelial cell proliferation with accumulation of p27 protein in the mouse uterus. Thus, suppression of ERβ function in the uterine epithelium during the neonatal period may be responsible for a risk for proliferative disease in adults.

show abstract

Estrogen receptor (ER) and its messenger ribonucleic acid expression in the genital tract of female mice exposed neonatally to tamoxifen and diethylstilbestrol

Cited by 47 publications

References 46 publications

Tamoxifen affects glucose and lipid metabolism parameters, causes browning of subcutaneous adipose tissue and transient body composition changes in C57BL/6NTac mice

Tamoxifen affects glucose and lipid metabolism parameters, causes browning of subcutaneous adipose tissue and transient body composition changes in C57BL/6NTac mice

Ontogeny of Estrogen Receptor Messenger Ribonucleic Acid Expression in the Postnatal Rat Uterus1

Neonatal Estrogen Receptor β Is Important in the Permanent Inhibition of Epithelial Cell Proliferation in the Mouse Uterus

Contact Info

Product

Resources

About