Estrogen receptor expression in lumbar intervertebral disc of the elderly: Gender- and degeneration degree-related variations

Song, Xiaoxin; Yu, Yong-juan; Li, Xin-feng; Liu, Zude; Yu, Bo; Guo, Zhen

doi:10.1016/j.jbspin.2013.09.002

Cited by 35 publications

(29 citation statements)

References 24 publications

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“…Immunohistochemistry was performed according to our previously described procedure [56, 57]. Paraffin sections (5μm thick) of the IVD tissue were treated with xylene to remove paraffin and rehydrated in graded alcohol baths followed by three rinses with PBS.…”

Section: Methodsmentioning

confidence: 99%

Estrogen receptors involvement in intervertebral discogenic pain of the elderly women: colocalization and correlation with the expression of Substance P in nucleus pulposus

et al. 2017

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Estrogenic modulation of pain is an exceedingly complex phenomenon. However, whether estrogen is involved in discogenic low back pain still remains unclear. Here, immunoreactivity staining technique was used to examine the expression level of the estrogen receptors (ERα and ERβ) and a pain related neuropeptide, Substance P in the lumbar intervertebral discs to analyze the relationship between the ERs and Substance P. Nucleus pulposus tissues of 23 elderly female patients were harvested during spinal surgeries and made to detect the immunoreactivity staining of ERα, ERβ and Substance P. The colocalization and intensities of ERs and Substance P were explored and evaluated respectively. The correlations between changes of ERα, ERβ and Substance P were also assessed.Our results revealed that Substance P colocalized with ERα and ERβ both in cytoplasm and nucleus of the nucleus pulposus cells. HSCORE analysis indicated that Substance P negatively correlated with both ERα and ERβ expression. Collectively, the crosstalk between ERs and Substance P might exist in the disc tissue. Estrogen-dependent pain mechanism might partly be mediated through ERs and Substance P in the nucleus pulposus of the elderly females. Estrogen and its receptors might be drug targets in discogenic low back pain diseases.

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Section: Methodsmentioning

confidence: 99%

Estrogen receptors involvement in intervertebral discogenic pain of the elderly women: colocalization and correlation with the expression of Substance P in nucleus pulposus

et al. 2017

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“…Some investigations have reported the expression of ERα and ERβ in all zones of the growth plate in humans as well as rats 4-6. However, it has been indicated that ERβ can antagonize ERα activity with a “yin yang” association between them in some species and tissues.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Estradiol on the Growth Plate Chondrocytes of Limb and Spine from Postnatal Mice in vitro: The Role of Estrogen-Receptor and Estradiol Concentration

Shi

Zheng

et al. 2017

Int. J. Biol. Sci.

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Objectives: Skeletal development is a complex process. Little is known about the different response of limb or spine growth plate chondrocytes (LGP or SGP) to the estrogen level and the role of estrogen receptor (ER) during postnatal stage.Methods: LGP and SGP chondrocytes were isolated from 50 one-week mice and treated with different concentrations of 17β-estradiol. Cell viability was measured by cell counting kit-8 (CCK-8). The expression of collagen II and X were evaluated by real-time PCR and Western blotting. Then, the response of LGP or SGP chondrocyte after with or without estradiol and specific ER antagonists to block the effect of ERs were also measured by Western blotting and immunofluorescence.Results: Estradiol promoted the chondrogensis of the chondrocytes in vitro and achieved the maximal expression of type II collagen at the dose of 10-7 M. Additionally, the regulatory effect of estradiol on the chondrogenesis can be mainly relied on ERα. The LGP chondrocytes were more sensitive to the estradiol treatment than SGP in the expression of type II collagen.Conclusions: Estrogen at a pharmacological concentration (10-7 M) could stimulate the maximal production of type II collagen in the growth plate chondrocytes in vitro, which exerts its activity mainly through ERα in the chondrogenesis. Furthermore, the LGP chondrocytes were more sensitive to the estradiol treatment than SGP in the chondrogenesis.

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“…Besides, though estrogen can perform functions through E which expressed in disc NP tissue in both men and women, care should be taken when the present data were applied to potential clinical interest for both male and female patients since the gender differences in the expression of E in NP cells [18]. The protective effects of estrogen on matrix synthesis of NP cells may indirectly provide explanations to the ef iciency of estrogen-replacement in retarding the disc height in post-menopausal women.…”

Section: Discussionmentioning

confidence: 92%

“…These positive effects of E2 on NP cells were in line with that on articular chondrocytes, and indicate that E2 can promote matrix synthesis potential of disc NP cells. E is also expressed in the disc tissue and down-regulated with advancing disc degeneration [17,18]. To investigate the role of E in the effects of E2, the speci ic E antagonist PHTPP and E agonist E B 1 were used to inhibit and activate E function.…”

Section: Discussionmentioning

confidence: 99%

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Estrogen Enhances Matrix Synthesis in Nucleus Pulposus Cell through the Estrogen Receptor β-p38 MAPK Pathway

Li¹,

Xu²,

Ye³

et al. 2016

Cell Physiol Biochem

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Background/Aims: Matrix homeostasis within the disc nucleus pulposus (NP) tissue is important for disc function. Increasing evidence indicates that sex hormone can influence the severity of disc degeneration. This study was aimed to study the role of 17β-estradiol (E₂) in NP matrix synthesis and its underlying mechanism. Methods: Rat NP cells were cultured with (10^-5, 10^-7 and 10^-9 M) or without (control) E2 for48 hours. The estrogen receptor (ER)-β antagonist PHTPP and ERβ agonist ERB 041 were used to investigate the role mediated by ERβ. The p38 MAPK inhibitor SB203580 was used to investigate the role of p38 MAPK signaling pathway. Gene and protein expression of SOX9, aggrecan and collagen II, glycosaminoglycan (GAG) content, and immunostaining assay for aggrecan and collagen II were analyzed to evaluate matrix production in rat NP cells. Results: E2 enhanced NP matrix synthesis in a concentration-dependent manner regarding gene and proetin expression of SOX9, aggrecan and collagen II, protein deposition of aggrecan and collagen II, and GAG content. Moreover, activation of p38 MAPK signaling pathway was increased with elevating E2 concentration. Further analysis indicated that ERB 041 and PHTPP could respectively enhance and suppress effects of E2 on matrix synthesis in NP cells, as well as activation of p38 MAPK pathway. Additionally, inhibition of p38 MAPK signaling pathway significantly abolished the effects of E2 on matrix synthesis. Conclusion: E2 can enhance matrix synthesis of NP cells and the ERβ/p38 MAPK pathway is involved in this regulatory process.

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Estrogen receptor expression in lumbar intervertebral disc of the elderly: Gender- and degeneration degree-related variations

Cited by 35 publications

References 24 publications

Estrogen receptors involvement in intervertebral discogenic pain of the elderly women: colocalization and correlation with the expression of Substance P in nucleus pulposus

Estrogen receptors involvement in intervertebral discogenic pain of the elderly women: colocalization and correlation with the expression of Substance P in nucleus pulposus

The Effect of Estradiol on the Growth Plate Chondrocytes of Limb and Spine from Postnatal Mice in vitro: The Role of Estrogen-Receptor and Estradiol Concentration

Estrogen Enhances Matrix Synthesis in Nucleus Pulposus Cell through the Estrogen Receptor β-p38 MAPK Pathway

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