Estrogen Regulates Amiloride-Binding Protein 1 through CCAAT/Enhancer-Binding Protein-β in Mouse Uterus during Embryo Implantation and Decidualization

Abstract

Embryo implantation is an intricate interaction between receptive uterus and active blastocyst. The mechanism underlying embryo implantation is still unknown. Although histamine and putrescine are important for embryo implantation and decidualization, excess amount of histamine and putrescine is harmful. Amiloride binding protein 1 (Abp1) is a membrane-associated amine oxidase and mainly metabolizes histamine and putrescine. In this study, we first showed that Abp1 is strongly expressed in the decidua on d 5-8 of pregnancy. Abp1 expression is not detected during pseudopregnancy and under delayed implantation but is detected after estrogen activation. Because Abp1 is mainly localized in the decidua and also strongly expressed during in vitro decidualization, Abp1 might play a role during mouse decidualization. The regulation of estrogen on Abp1 is mediated by transcription factor CCAAT/enhancer-binding protein-β. Abp1 expression is also regulated by cAMP, bone morphogenetic protein 2, and ERK1/2. Abp1 may be essential for mouse embryo implantation and decidualization.

Differential expression and regulation of Cryab in mouse uterus during preimplantation period

“…A previous study found that cAMP levels significantly increased after sesame oil stimulation of the uterine horn (Rankin et al 1977). Moreover, cAMP could induce and regulate the decidualization of uterine stromal cells (Gellersen & Brosens 2003, Liang et al 2010. In mouse uterine stromal cells cultured in vitro, we found that cAMP induced the expression of the Cryab gene, which further supports a role for Cryab in decidualization.…”

“…Uterine stromal cells were isolated as previously reported (Liang et al 2010). Briefly, uterine horns from day 4 pregnant mice were split longitudinally to expose the uterine lumen and digested in Hanks' balanced salt solution (HBSS) containing 1% trypsin (Amresco, Solon, OH, USA) and 6 mg/ml dispase (Roche).…”

“…In vitro decidualization of endometrial stromal cells from day 4 of pregnancy was performed as described previously (Liang et al 2010). Briefly, primary uterine stromal cells isolated on day 4 pregnancy were induced for in vitro decidualization with fresh medium supplemented with progesterone (100 nM) and estrogen (0.1 nM) in DMEM-F12 with 2% charcoal-treated FBS (Biological Industries Ltd., Kibbutz Beit Hemeek, Israel).…”

Amine Oxidase Copper-containing 1 (AOC1) Is a Downstream Target Gene of the Wilms Tumor Protein, WT1, during Kidney Development

“…The proximal AOC1 promoter * This work was supported by Deutsche Forschungsgemeinschaft Grant region is GC-rich and displays no TATA box, CAAT box, or consensus initiator sequence (16). Liang et al (26) reported that estrogen can induce AOC1 expression via CCAAT/enhancerbinding protein-␤ in mouse uterus during embryo implantation and decidualization. Other putative trans-acting factors include cyclic AMP, bone morphogenetic protein 2, and ERK1/2 (26).…”

“…Liang et al (26) reported that estrogen can induce AOC1 expression via CCAAT/enhancerbinding protein-␤ in mouse uterus during embryo implantation and decidualization. Other putative trans-acting factors include cyclic AMP, bone morphogenetic protein 2, and ERK1/2 (26).…”